LOCUS       sequence-236966-        8090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional Unit (TU) for YFP expression, obtained by combining 
            FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to 
            FungalBraid/GoldenBraid modular DNA assembly for ATMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB026
FEATURES             Location/Qualifiers
     source          1..8090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..87
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    419..443
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             876..1691
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             876..1691
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(949..968)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1779..2367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2268..2287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2521..2538
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2553..2693
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2619..2638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2779..2801
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3037..3231
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3297..4370)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4799..5428)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6729..6753
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(7503..7505,7506..7508,7509..8021)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(7548..7569)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7809..7828)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             join(7968..8090,1..129)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
ORIGIN
        1 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
       61 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      121 ctctacaagt cagtcatgag ctgagcttac ttaacgttac tgaaatcatc aaacagcttg
      181 acgaatctgg atataagatc gttggtgtcg atgtcagctc cggagttgag acaaatggtg
      241 ttcaggatct cgataagata cgttcatttg tccaagcagc aaagagtgcc ttctagtgat
      301 ttaatagctc catgtcaaca agaataaaac gcgtttcggg tttacctctt ccagatacag
      361 ctcatctgca atgcatcgac cgtctgagac atgcgctctc ctgagacgga tccgttatgt
      421 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
      481 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
      541 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
      601 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
      661 gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata
      721 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
      781 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
      841 ctgtagaaaa gaggaaggaa ataataagtt ctgttatgag ccatattcaa cgggaaacgt
      901 cttgctcaag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      961 ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg
     1021 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1081 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1141 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
     1201 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1261 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgac
     1321 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     1381 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     1441 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1501 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1561 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1621 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1681 agtttttcta atcacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1741 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1801 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1861 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1921 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1981 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2041 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2101 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2161 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2221 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2281 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2341 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2401 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2461 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2521 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     2581 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     2641 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2701 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2761 gacaagctgt gaccgtcacc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2821 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     2881 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     2941 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     3001 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     3061 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     3121 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     3181 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagagt
     3241 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     3301 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     3361 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     3421 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     3481 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     3541 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     3601 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     3661 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     3721 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     3781 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     3841 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     3901 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
     3961 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
     4021 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
     4081 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
     4141 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
     4201 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
     4261 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
     4321 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
     4381 cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg
     4441 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
     4501 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
     4561 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
     4621 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
     4681 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
     4741 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
     4801 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
     4861 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
     4921 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
     4981 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
     5041 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
     5101 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
     5161 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
     5221 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
     5281 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
     5341 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
     5401 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
     5461 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
     5521 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
     5581 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagaggt gcgcgggcgg
     5641 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
     5701 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
     5761 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
     5821 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     5881 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     5941 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     6001 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     6061 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     6121 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     6181 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
     6241 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
     6301 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
     6361 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
     6421 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     6481 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     6541 ttttcagatg acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     6601 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
     6661 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
     6721 tctcttaggt ttacccgcca atatatcctg tcaggatccg tctcatgacg gagtggcgca
     6781 tgcggacaga cggacggacg cagagagaag ggctgagtaa taagcgccac tgcgccagac
     6841 agctctggcg gctctgaggt gcagtggatg attattaatc cgggaccggc cgcccctccg
     6901 ccccgaagtg gaaaggctgg tgtgcccctc gttgaccaag aatctattgc atcatcggag
     6961 aatatggagc ttcatcgaat caccggcagt aagcgaagga gaatgtgaag ccaggggtgt
     7021 atagccgtcg gcgaaatagc atgccattaa cctaggtaca gaagtccaat tgcttccgat
     7081 ctggtaaaag attcacgaga tagtaccttc tccgaagtag gtagagcgag tacccggcgc
     7141 gtaagctccc taattggccc atccggcatc tgtagggcgt ccaaatatcg tgcctctcct
     7201 gctttgcccg gtgtatgaaa ccggaaaggc cgctcaggag ctggccagcg gcgcagaccg
     7261 ggaacacaag ctggcagtcg acccatccgg tgctctgcac tcgacctgct gaggtccctc
     7321 agtccctggt aggcagcttt gccccgtctg tccgcccggt gtgtcggcgg ggttgacaag
     7381 gtcgttgcgt cagtccaaca tttgttgcca tattttcctg ctctccccac cagctgctct
     7441 tttcttttct ctttcttttc ccatcttcag tatattcatc ttcccatcca agaaccttta
     7501 aaatggtgag caagggtgaa gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     7561 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gacgccacct
     7621 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     7681 ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc gaccacatga
     7741 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7801 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7861 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7921 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7981 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     8041 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
//
