LOCUS       sequence-236989-       10915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMA7CR_2.0 contains arabinose inducible lambda/RED beta-protein and 
            dam, and aTc inducible CRISPR/Cas9 and recX.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Ronda C, Pedersen LE, Sommer MO, Nielsen AT
  TITLE     CRMAGE: CRISPR Optimized MAGE Recombineering.
  JOURNAL   Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
  PUBMED    26797514
REFERENCE   2  (bases 1 to 10915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-01-22"; volume: "6:19452. doi"; pages: " 
            10.1038/srep19452"
COMMENT     Sequence Label: pMA7CR_2.0
FEATURES             Location/Qualifiers
     source          1..10915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..18)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      171..257
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      349..376
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    395..413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             453..464
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             985..5091
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             985..5088
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        5166..5257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5258..5326,5327..6118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5476..5495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6160..6615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6247..6266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6457..6478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      6726..7314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7215..7234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7468..7485
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7651..7673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7811..7830
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(8010..8888)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        8915..9199
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     9122..9141
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             9234..10019
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MGTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
       61 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
      121 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
      181 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      241 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
      301 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
      361 tgacggatgg cctttttgcg tttctactcc ctattcccta tcagtgatag agatactgag
      421 cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc atgacagaat
      481 caacatcccg tcgcccggca tatgctcgcc tgttggatcg tgcggtacgc attctggcgg
      541 tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc ggcaccgatt atgggcaaaa
      601 atggcccaga agagattgat gctacggcag aagattacga gcgcgttatt gcctggtgcc
      661 atgaacatgg ctatctcgat gacagccgat ttgttgcgcg ctttatcgcc agccgtagcc
      721 gcaaaggtta tggacctgcg cgtattcgcc aggaactgaa tcagaaaggt atttcccgcg
      781 aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg gtgcgcactg gcgcgcgatc
      841 aggcgacgcg aaaatatggc gaacctttgc caactgtctt ttcagaaaaa gttaagatcc
      901 agcgttttct gctctatcgt ggctatctga tggaagatat ccaggagatt tggcgaaatt
      961 ttgccgactg agaaggagat atacatggat aagaaatact caataggctt agatatcggc
     1021 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     1081 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     1141 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     1201 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     1261 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     1321 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     1381 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     1441 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     1501 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     1561 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     1621 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     1681 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     1741 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     1801 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     1861 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     1921 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     1981 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     2041 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     2101 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     2161 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     2221 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     2281 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     2341 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     2401 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     2461 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     2521 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     2581 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     2641 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     2701 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     2761 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     2821 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     2881 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     2941 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     3001 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     3061 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     3121 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     3181 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     3241 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     3301 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     3361 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     3421 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     3481 ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt tccttaaaga cgattcaata
     3541 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     3601 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     3661 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     3721 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     3781 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     3841 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     3901 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     3961 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     4021 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     4081 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     4141 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     4201 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     4261 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
     4321 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     4381 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     4441 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
     4501 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     4561 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     4621 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     4681 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     4741 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     4801 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     4861 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     4921 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     4981 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     5041 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgactg agatcccatg
     5101 gtacgcgtgc tagaggcatc aatgtgaaaa agcccgcgca agcgggtttt tttatgacaa
     5161 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5461 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     5521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6121 gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt agcggcgcat
     6181 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6241 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6301 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6361 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6421 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6481 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     6541 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6601 taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6841 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     6901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7081 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7141 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7201 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7261 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7321 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7381 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7441 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7501 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtcatgg
     7561 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     7621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     7681 cgtcatcacc gaaacgcgcg aggcagaagg agatggcgcc caacagtccc ccggccacgg
     7741 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     7801 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     7861 tgccggccac gatgcgtccg gcgtagagga tctgctcatg tttgacagct tatcatcgat
     7921 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
     7981 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
     8041 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
     8101 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
     8161 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
     8221 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
     8281 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
     8341 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
     8401 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
     8461 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
     8521 tccgggcgaa agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag
     8581 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
     8641 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
     8701 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
     8761 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
     8821 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
     8881 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
     8941 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc ggtaaccccg
     9001 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
     9061 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
     9121 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     9181 ctctctactg tttctccata cccgtttttt tgggctagca ggaggaattc accatgggta
     9241 ctgcactcgc aacgctggct gggaagctgg ctgaacgtgt cggcatggat tctgtcgacc
     9301 cacaggaact gatcaccact cttcgccaga cggcatttaa aggtgatgcc agcgatgcgc
     9361 agttcatcgc attactgatc gttgccaacc agtacggcct taatccgtgg acgaaagaaa
     9421 tttacgcctt tcctgataag cagaatggca tcgttccggt ggtgggcgtt gatggctggt
     9481 cccgcatcat caatgaaaac cagcagtttg atggcatgga ctttgagcag gacaatgaat
     9541 cctgtacatg ccggatttac cgcaaggacc gtaatcatcc gatctgcgtt accgaatgga
     9601 tggatgaatg ccgccgcgaa ccattcaaaa ctcgcgaagg cagagaaatc acggggccgt
     9661 ggcagtcgca tcccaaacgg atgttacgtc ataaagccat gattcagtgt gcccgtctgg
     9721 ccttcggatt tgctggtatc tatgacaagg atgaagccga gcgcattgtc gaaaatactg
     9781 catacactgc agaacgtcag ccggaacgcg acatcactcc ggttaacgat gaaaccatgc
     9841 aggagattaa cactctgctg atcgccctgg ataaaacatg ggatgacgac ttattgccgc
     9901 tctgttccca gatatttcgc cgcgacattc gtgcatcgtc agaactgaca caggccgaag
     9961 cagtaaaagc tcttggattc ctgaaacaga aagccgcaga gcagaaggtg gcagcatgaa
    10021 gtcggtgtgg agtgaaacga tgaagaaaaa tcgcgctttt ttgaagtggg cagggggcaa
    10081 gtatcccctg cttgatgata ttaaacggca tttgcccaag ggcgaatgtc tggttgagcc
    10141 ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt tctcgttata tccttgccga
    10201 tatcaatagc gacctgatca gtctctataa cattgtgaag atgcgtactg atgagtacgt
    10261 acaggccgca cgcgagctgt ttgttcccga aacaaattgc gccgaggttt actatcagtt
    10321 ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg gcggtactgt ttttatattt
    10381 gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg cgcggtgagt ttaacgtgcc
    10441 gttcggccgc tacaaaaaac cctatttccc ggaagcagag ttgtatcact tcgctgaaaa
    10501 agcgcagaat gcctttttct attgtgagtc ttacgccgat agcatggcgc gcgcagatga
    10561 tgcatccgtc gtctattgcg atccgcctta tgcaccgctg tctgcgaccg ccaactttac
    10621 ggcgtatcac acaaacagtt ttacgcttga acaacaagcg catctggcgg agatcgccga
    10681 aggtctggtt gagcgccata ttccagtgct gatctccaat cacgatacga tgttaacgcg
    10741 tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt cgacgcagta taagcagcaa
    10801 cggcggcaca cgtaaaaagg tggacgaact gctggctttg tacaaaccag gagtcgtttc
    10861 acccgcgaaa aaataataag cttggctgtt ttggcggatg agagaagatt ttcag
//
