LOCUS       sequence-237175-        5045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FMN-based fluorescent protein Reporter plasmid for B-Rex contain 
            imperfect operator sequence (anaerobic purpose).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Liu Y, Landick R, Raman S
  TITLE     A Regulatory NADH/NAD+ Redox Biosensor for Bacteria.
  JOURNAL   ACS Synth Biol. 2019 Feb 15;8(2):264-273. doi: 
            10.1021/acssynbio.8b00485. Epub 2019 Jan 23.
  PUBMED    30633862
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00485"; journalName: "ACS Synth Biol"; date: 
            "2019-02-15- 15"; volume: "8"; issue: "2"; pages: "264-273"
COMMENT     Sequence Label: pROP-IP-FBFP
FEATURES             Location/Qualifiers
     source          1..5045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      782..876
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1368..1590
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1638..2588
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             3165..3956
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVITEVSTQLSEVVGAIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(4061..4147)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4762..4780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      4851..4880
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     promoter        4973..5007
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
ORIGIN
        1 atggtttcca agggcgagga ggataacatg gctatcatta aagagttcat gcgcttcaaa
       61 gttcacatgg agggttctgt taacggtcac gagttcgaga tcgaaggcga aggcgagggc
      121 cgtccgtatg aaggcaccca gaccgccaaa ctgaaagtga ctaaaggcgg cccgctgcct
      181 tttgcgtggg acatcctgag cccgcaattt atgtacggtt ctaaagcgta tgttaaacac
      241 ccagcggata tcccggacta tctgaagctg tcttttccgg aaggtttcaa gtgggaacgc
      301 gtaatgaatt ttgaagatgg tggtgtcgtg accgtcactc aggactcctc cctgcaggat
      361 ggcgagttca tctataaagt taaactgcgt ggtactaatt ttccatctga tggcccggtg
      421 atgcagaaaa agacgatggg ttgggaggcg tctagcgaac gcatgtatcc ggaagatggt
      481 gcgctgaaag gcgaaattaa acagcgcctg aaactgaaag atggcggcca ttatgacgct
      541 gaagtgaaaa ccacgtacaa agccaagaaa cctgtgcagc tgcctggcgc gtacaatgtg
      601 aatattaaac tggacatcac ctctcataat gaagattata cgatcgtaga gcaatatgag
      661 cgcgcggagg gtcgtcattc taccggtggc atggatgagc tgtaaaataa cagggtggtg
      721 acaccttgcc cgtttttttg ccggagctag agctgtcaga ccaagtttac gagctcgctt
      781 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg
      841 ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gcactaggga cagtaagacg
      901 ggtaagcctg ttgatgatac cgctgcctta ctgggtgcat tagccagtct gaatgacctg
      961 tcacgggata atccgaagtg gtcagactgg aaaatcagag ggcaggaact gctgaacagc
     1021 aaaaagtcag atagcaccac atagcagacc cgccataaaa cgccctgaga agcccgtgac
     1081 gggcttttct tgtattatgg gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg
     1141 ccatttaccc ccattcactg ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag
     1201 acagcgactc aggtgcctga tggtcggaga caaaaggaat attcagcgat ttgcccgagc
     1261 ttgcgagggt gctacttaag cctttagggt tttaaggtct gttttgtaga ggagcaaaca
     1321 gcgtttgcga catccttttg taatactgcg gaactgacta aagtagtgag ttatacacag
     1381 ggctgggatc tattcttttt atcttttttt attctttctt tattctataa attataacca
     1441 cttgaatata aacaaaaaaa acacacaaag gtctagcgga atttacagag ggtctagcag
     1501 aatttacaag ttttccagca aaggtctagc agaatttaca gatacccaca actcaaagga
     1561 aaaggactag taattatcat tgactagccc atctcaattg gtatagtgat taaaatcacc
     1621 tagaccaatt gagatgtatg tctgaattag ttgttttcaa agcaaatgaa ctagcgatta
     1681 gtcgctatga cttaacggag catgaaacca agctaatttt atgctgtgtg gcactactca
     1741 accccacgat tgaaaaccct acaaggaaag aacggacggt atcgttcact tataaccaat
     1801 acgctcagat gatgaacatc agtagggaaa atgcttatgg tgtattagct aaagcaacca
     1861 gagagctgat gacgagaact gtggaaatca ggaatccttt ggttaaaggc tttgagattt
     1921 tccagtggac aaactatgcc aagttctcaa gcgaaaaatt agaattagtt tttagtgaag
     1981 agatattgcc ttatcttttc cagttaaaaa aattcataaa atataatctg gaacatgtta
     2041 agtcttttga aaacaaatac tctatgagga tttatgagtg gttattaaaa gaactaacac
     2101 aaaagaaaac tcacaaggca aatatagaga ttagccttga tgaatttaag ttcatgttaa
     2161 tgcttgaaaa taactaccat gagtttaaaa ggcttaacca atgggttttg aaaccaataa
     2221 gtaaagattt aaacacttac agcaatatga aattggtggt tgataagcga ggccgcccga
     2281 ctgatacgtt gattttccaa gttgaactag atagacaaat ggatctcgta accgaacttg
     2341 agaacaacca gataaaaatg aatggtgaca aaataccaac aaccattaca tcagattcct
     2401 acctacataa cggactaaga aaaacactac acgatgcttt aactgcaaaa attcagctca
     2461 ccagttttga ggcaaaattt ttgagtgaca tgcaaagtaa gtatgatctc aatggttcgt
     2521 tctcatggct cacgcaaaaa caacgaacca cactagagaa catactggct aaatacggaa
     2581 ggatctgagg ttcttatggc tcttgtatct atcagtgaag catcaagact aacaaacaaa
     2641 agtagaacaa ctgttcaccg ttacatatca aagggaaaac tgtccatatg cacagatgaa
     2701 aacggtgtaa aaaagataga tacatcagag cttttacgag tttttggtgc attcaaagct
     2761 gttcaccatg aacagatcga caatgtaact actagagctg atccttcaac tcagcaaaag
     2821 ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
     2881 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2941 gtgttatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggtg
     3001 cagtggcggt tttcatggct tgctatgact gtttttttgg ggtacagtct atgcctcggg
     3061 catccaagca gcaagcgcgt tacgccatgg gtcgatgttt gatgttatgg agcagcaacg
     3121 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     3181 tcaccgaagt atcgactcaa ctatcagagg tagttggcgc catcgagcgc catctcgaac
     3241 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3301 gtgatattga tttgctagtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3361 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3421 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3481 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3541 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3601 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3661 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3721 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3781 atgtcgctgc cgactgggca atggagcgcc tgccggccca atatcagccc gtcatacttg
     3841 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3901 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataataat
     3961 actagcagaa atcatcctta gcgaaagcta aggatttttt ttatctgatt accgcctttg
     4021 agtgagcccc aatgataacc ccctaggtct agggcggcgg atttgtccta ctcaggagag
     4081 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     4141 ttatttgatg cctctagcac gcgtaccatg ggatcccccg ggctgcagga attcgatatc
     4201 aagcttttac tccaacagct tctcgtactc cttctgtttg gtgatgtcgt tctgaatacc
     4261 aacaaagtac gtcttatcct caatctccat cgggtcaatg ttcagttcat tccagaacat
     4321 cgtgccgtct tttttatagt tctggatctg gaccgtaacc ggttctttat tttgcagcgc
     4381 ggtgcgaatg ttatcgacct ctgccgggtc ggtgtgtttg ccttgcagga agcgtgcatt
     4441 tttgcctaag atttcttccg tttcgtagcc ggtcatttgg acgaagccct gattcacata
     4501 cacaatcggg ttatcttcca gggccggatc ggtgataacg acgccaacgc gcacatgatc
     4561 cagcgccttc ttaatcacct ccagctgacc cggaatacca aaagactgaa aggacgccat
     4621 gctataaacc tcctaataga ttttataaga attgtgaatt ctttttgctc aatatttcac
     4681 atggctgtaa taatacctag gactgagcta gctgtaaata ctcttttttt gctagcatct
     4741 cgaggtgaag acgaaaaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4801 taataatggt ttcttagacg tcggaattgt tacgtagcaa tcaactcact ggctcacctt
     4861 cacgggtggg cctttcttcg gcacgggcaa attgctgaat attccttttc ttagacgtca
     4921 ggtggcaaac gcgaagtaat cttttcggtt ttaaagaaaa agggggcgcg cctttacggc
     4981 tagctcagcc ctaggtatta tgctagcatg gagcaagaag gtaagcgagg ataagaagga
     5041 aaaga
//
