LOCUS       sequence-237178-        9204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-Control
FEATURES             Location/Qualifiers
     source          1..9204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     329..352
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        399..1150
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             1157..2809
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2852..2869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2858..3082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3318..3362)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3547..3780
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4273..4398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4445..4625
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4626..4852)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4880..4900)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4880..4898)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4919..4935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4919..4935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4932..4954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4943..4959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4967..4973,4974..4991,4992..4997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5012..5033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5150..5167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5321..5909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5401..5420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6080..6871,6872..6940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6703..6722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6941..7045)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7071..7526)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7208..7229)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7420..7439
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7653..7675
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7667..7684
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7668..7684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7694..7713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7694..7712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7733..7868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7908..8029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7919..7938)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7973..7992
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8101..8334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8472..9071)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8555..8575)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9052..9071
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9092..9204,1..387))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9140..9159)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg
       61 agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc
      121 tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg
      181 cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg
      241 ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc
      301 agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact
      361 gccttgggaa aagcgcctcc cctacccggt agaattgaaa tgagtcttcg gacctcgcgg
      421 gggccgctta agcggtggtt agggtttgtc tgacgcgggg ggagggggaa ggaacgaaac
      481 actctcattc ggaggcggct cggggtttgg tcttggtggc cacgggcacg cagaagagcg
      541 ccgcgatcct cttaagcacc cccccgccct ccgtggaggc gggggtttgg tcggcgggtg
      601 gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac cttttcggtc
      661 tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg gtcctaggct
      721 ccatggggac cgtatacgtg gacaggctct ggagcatccg cacgactgcg gtgatattac
      781 cggagacctt ctgcgggacg agccgggtca cgcggctgac gcggagcgtc cgttgggcga
      841 caaacaccag gacggggcac aggtacacta tcttgtcacc cggaggcgcg agggactgca
      901 ggagcttcag ggagtggcgc agctgcttca tccccgtggc ccgttgctcg cgtttgctgg
      961 cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc
     1021 ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccca
     1081 ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg
     1141 acccgcttaa ggatccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
     1201 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
     1261 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
     1321 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
     1381 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
     1441 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
     1501 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
     1561 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
     1621 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
     1681 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
     1741 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     1801 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     1861 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     1921 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     1981 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     2041 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     2101 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     2161 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     2221 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     2281 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     2341 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     2401 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     2461 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     2521 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     2581 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     2641 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     2701 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     2761 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat tctagttgtt
     2821 tctagagtcg acgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     2881 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2941 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3001 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3061 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3121 tatcccctcc ccccttttct tttgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     3181 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     3241 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     3301 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     3361 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     3421 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     3481 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     3541 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     3601 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     3661 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     3721 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     3781 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     3841 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     3901 atatctcctc ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt
     3961 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     4021 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     4081 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     4141 gccaggatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     4201 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     4261 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     4321 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     4381 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     4441 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     4501 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     4561 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     4621 gacccgttta tgtatcgagc taggcactta aatacaatat ctctgcaatg cggcaattca
     4681 gtggttcgtc caatccatgt cagacccgtc tgttgccttc ctaataaggc acgatcgtac
     4741 caccttactt ccaccaatcg gcatgcacgg tgctttttct ctccttgtaa ggcatgttgc
     4801 taactcatcg ttaccatgtt gcaagactac aagagtattg cataagacta cattaagctt
     4861 gcagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4921 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4981 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5041 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5101 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5761 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     7081 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7141 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7201 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7261 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7321 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7381 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7441 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7501 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     7561 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     7621 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7681 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtacgtccc taggcctcca
     7741 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     7801 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     7861 ggcgggatgg gcggagttag gggcgggata gctagagcca gacatgataa gatacattga
     7921 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     7981 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc ctctcactct
     8041 ctgatattca tttctttgca agttataaat actgaataat aagatgacat gaactactac
     8101 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     8161 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     8221 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     8281 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg agctagccct tccagtcccc
     8341 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     8401 gtaccgggcc ctcgagatgc atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg
     8461 tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
     8521 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
     8581 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
     8641 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
     8701 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
     8761 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
     8821 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
     8881 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
     8941 tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
     9001 gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
     9061 tactcggtca tggtaagctt gggctgcagg tcgaaaggcc cggagatgag gaagaggaga
     9121 acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc
     9181 gggcgcccgc cccgcccctg agcc
//
