LOCUS       sequence-237286-        4754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 
            1,4,5-trisphosphate 3-kinase A; clone CP16 (full length).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Takazawa K, Vandekerckhove J, Dumont JE, Erneux C
  TITLE     Cloning and expression in Escherichia coli of a rat brain cDNA 
            encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 
            3-kinase.
  JOURNAL   Biochem J. 1990 Nov 15;272(1):107-12.
  PUBMED    2176078
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            J."; date: "1990-11-15"; volume: "272(1)"; pages: "107-12"
COMMENT     Sequence Label: rat brain inositol 1,4,5-trisphosphate 3-kinase A
FEATURES             Location/Qualifiers
     source          1..4754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     191..207
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2036..2052)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2037..2053)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(2077..2096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2078..2096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2103..2120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2103..2119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2112..2134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(2261..2716)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2398..2419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2610..2629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2743..2847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2848..2916,2917..3708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3066..3085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4621..4638
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
      181 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattccgga ctcggctgcc
      241 gctccggtgt cgggagatga ccctgcccgg acacccgacg ggcatggcgc gaccacgggg
      301 cgcggggccc tgcagccccg ggttggagcg ggctccgcgc cggagcgtcg gggagctgcg
      361 cctgctcttc gaagcgcgct gcgccgcagt cgccgccgca gcggccgcag gggagccccg
      421 ggcccgcggg gccaagcggc gtgggggaca agtgcctaac gggctcccgc gtgctgcccc
      481 tgctccggtg atcccgcagc tcactgtgac aagcgaggag gatgtggccc cggccagccc
      541 cgggccgccg gaccgggagg ggaactggct cccagctgcg gggtcgcacc tgcagcagcc
      601 acgccgcctc tccacctcgt ccctctcctc caccggctcc tcgtcgctgc tcgaggactc
      661 ggaggacgac ctgctgagcg acagcgagag ccggagccgc ggcaacgtgc agctggaaac
      721 cagcgaagac gtggggcaga aaagccattg gcagaagatc cgtaccatgg tcaacctgcc
      781 tgtcatgagt cctttcaaaa agcgctactc ctgggtgcag ttagcagggc acacagggag
      841 tttcaaagct gcgggcacca gcggcctgat cctgaagcgc agctcggagc ctgaacacta
      901 ctgcctggtg cggttgatgg ctgatgtgct gcgtgggtgt gtgccggcct tccacggtgt
      961 ggtcgagcgg gatggtgaaa gctacttaca gttacaggac ctgcttgatg gcttcgatgg
     1021 accttgcgtg cttgactgca agatgggtgt cagaacttac ctggaagaag agctgaccaa
     1081 agcccgagaa cggcccaagc tgcgaaagga catgtataag aaaatgctgg ccgtggaccc
     1141 cgaggcacct actgaggagg agcatgcgca gcgtgccgtc acaaaacctc gatacatgca
     1201 atggcgcgaa ggcatcagct ccagcactac actcggcttc cgcatcgagg gcatcaagaa
     1261 agccgatgga tcttgcagta ctgatttcaa aactacacga agcagagagc aagtgactcg
     1321 agtctttgag gagttcatgc aaggagatgc tgaagtgctg aagaggtatc tgaaccgcct
     1381 acagcagatc cgggataccc tggagatctc tgatttcttt agacggcacg aggtgattgg
     1441 cagctcactc ctcttcgtgc atgaccattg ccatcgcgct ggtgtgtggc tcatcgattt
     1501 tggcaagacc acgcctctcc ccgatggcca gatcctggat catcggaggc cctgggagga
     1561 gggcaatcgt gaggacggct atttgctggg gctggacaat ctcattggca tcttggccaa
     1621 cctggctgag agatgaggct gggccctttt ccctcagctt gggccagctg gtcagagatg
     1681 tggacctgtg gctgcatccc caccatgcat gccggcaaga gactggaacc ccgctgggcg
     1741 ggcagttcac atggtcctgg aggaccaggt gccatccact aagggggtgt agtacattag
     1801 gagctggctt ctacacaagc ctgtctccca gagctgaatg acactaattt ataggagagg
     1861 aaggaaacag tgggcttctc ccccagcctt gtcaagtgac tttgcacctc tccagagggt
     1921 cccgatccag aattgtactt cacaggtata tagacttact ggtctaactt cctacactac
     1981 catggacacc cttcctaata aaactcaaag attcggaatt cgatatcaag cttatcgata
     2041 ccgtcgacct cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacaat
     2101 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2161 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc aaattgtaag cgttaatatt
     2281 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2341 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2401 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2461 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2521 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2581 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2641 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2701 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4621 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     4681 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     4741 gagcgcaacg caat
//
