LOCUS       sequence-23747-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Pfleger BF, Fawzi NJ, Keasling JD
  TITLE     Optimization of DsRed production in Escherichia coli: effect of 
            ribosome binding site sequestration on translation efficiency.
  JOURNAL   Biotechnol Bioeng. 2005 Dec 5. 92(5):553-8.
  PUBMED    16247774
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2005 Dec 5. 92(5):553-8."
COMMENT     Sequence Label: pBAD*RFPEC2
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(209..237)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acntcgngga gcagtacgag cgcgccgagg gccgccacca cctgttcctg tagatctgat
       61 cgttgcgggc ggggcgagag tctcgccccg cccgcgaccg cggtgaaaat acgagaatat
      121 ttttgtattg atctcctagg cggggtaccg tattttggat gataacgagg cgcaaaaaat
      181 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      361 cactactttc tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca
      421 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa
      481 agatgacggg aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa
      541 tcgtatcgag ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact
      601 ggagtacaac tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat
      661 caaagctanc ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacna
      721 ttatcaacaa aatactccat tggcgatggc ctgtccttta ccagaca
//
