LOCUS       sequence-237531-        9039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of mCitrine under the control of the Gal1 promoter and
            erg3 fused to mCherry (an ER-targeting marker protein) under the 
            control of the Gal10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pESC/mCitrine/erg3-mCherry
FEATURES             Location/Qualifiers
     source          1..9039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     177..196
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     436..457
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        516..1180
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(529..552)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    847..964
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             2322..3029
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2464..2482)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2732..2751
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3153..3318
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3208..3232)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     rep_origin      3397..3852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3484..3503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3694..3715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3983..4786)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4787..5002)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5026..5045)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5145..5167
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5205..5223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      5264..6606
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    5596..5643
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6633..6737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6738..6806,6807..7598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6956..6975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7769..8357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8258..8277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8511..8528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8600..8789)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(join(8822..9039,1..496,497..499,500..502))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEMFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8867..8888)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
       61 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      121 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      181 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      241 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cacatcaggc
      301 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      361 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      421 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
      481 tctcctcgcc cttgctcacc attgagggat ccggggtttt ttctccttga cgttaaagta
      541 tagaggtata ttaacaattt tttgttgata cttttattac atttgaataa gaagtaatac
      601 aaaccgaaaa tgttgaaagt attagttaaa gtggttatgc agtttttgca tttatatatc
      661 tgttaataga tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa
      721 actaatcgca ttatcatcct atggttgtta atttgattcg ttcatttgaa ggtttgtggg
      781 gccaggttac tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt
      841 attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga
      901 cgaggacgca cggaggagag tcttccttcg gagggctgtc acccgctcgg cggcttctaa
      961 tccgtacttc aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg
     1021 tttttttagg ctaagataat ggggctcttt acatttccac aacatataag taagattaga
     1081 tatggatatg tatatggata tgtatatggt ggtaatgcca tgtaatatga ttattaaact
     1141 tctttgcgtc catccaaaaa aaaagtaaga atttttgaaa attcgaattc aaccctcact
     1201 aaagggcggc catggatttg gtcttagaag tcgctgacca ttatgtctta gacgacttgt
     1261 acgctaaagt tctgcccgct tcgttggcag ccaatattcc tgtcaagtgg cagaaattgc
     1321 tagggttgaa cagtgggttc agcaattcta cgattttgca ggagactttg aactccaaga
     1381 atgccgtcaa agaatgtaga aggttctacg ggcaggtgcc attcctgttt gatatgtcga
     1441 cgacgtcttt tgcatcgcta ttgcctcgtt ccagcatctt gagagaattc ctctcactat
     1501 gggttattgt tacgatcttt ggtttactac tttacttatt cacggctagt ctcagctacg
     1561 tgtttgtgtt tgacaagtcg attttcaacc atcctcgtta cttgaaaaac caaatggcaa
     1621 tggaaatcaa gttggcagtc agtgctatcc catggatgtc gatgttgacc gctccatggt
     1681 ttgttatgga attgaacggc cattctaaac tatacatgaa gattgattat gaaaaccacg
     1741 gtgtaaggaa gctcattatc gagtacttca ctttcatctt tttcactgat tgcggtgtgt
     1801 atttagcgca cagatggttg cattggccaa gggtctaccg tgctctgcac aagcctcatc
     1861 acaagtggct ggtctgcaca cctttcgcat ctcattcttt ccatcctgta gacgggtttt
     1921 tgcaatccat ctcgtaccac atctacccat tgattctgcc gttacacaag gtttcttatt
     1981 tgattctgtt cacttttgtt aacttttgga ctgttatgat tcatgacggt caatacctat
     2041 caaacaatcc tgccgtcaac ggtactgcct gccacacggt tcaccatcta tatttcaact
     2101 acaactacgg tcaattcacc actttgtggg acagactagg gggttcttac cgtagaccag
     2161 atgactcatt gtttgatcct aagttaagag atgctaagga gacctgggac gctcaagtta
     2221 aggaagttga acatttcatc aaggaggtcg aaggtgatga taatgataga atctatgaaa
     2281 acgacccaaa taccaagaag aacaacggag gtggtggatc tgtgagcaag ggcgaggagg
     2341 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     2401 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     2461 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     2521 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     2581 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     2641 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     2701 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     2761 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     2821 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2881 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     2941 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3001 ccggcggcat ggacgagctg tacaagtaat tcgccagagg tttggtcaag tctccaatca
     3061 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     3121 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     3181 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     3241 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     3301 tatagcatga ggtcgctcca attcagctgg cgtaatagcg aagaggcccg caccgatcgc
     3361 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa
     3421 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3601 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3721 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3781 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3841 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3901 catagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg
     3961 catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc
     4021 agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc
     4081 ctcttccaac aataataatg tcagatcctg tagagaccac atcatccacg gttctatact
     4141 gttgacccaa tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat
     4201 cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt
     4261 tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct
     4321 tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg
     4381 cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg
     4441 cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt
     4501 cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg
     4561 gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac
     4621 aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca
     4681 atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag
     4741 gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt
     4801 tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt
     4861 caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct
     4921 tctgttcgga gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat
     4981 aaaaaaaaaa tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga
     5041 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5101 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5161 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5221 atttttatag gttaatgtca tgataataat ggtttcttag tatgatccaa tatcaaagga
     5281 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
     5341 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
     5401 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
     5461 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     5521 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     5581 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     5641 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     5701 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     5761 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     5821 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     5881 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     5941 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     6001 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     6061 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     6121 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     6181 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     6241 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     6301 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     6361 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     6421 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     6481 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     6541 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     6601 ttcgttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8581 ttcattaatg cagctggatc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta
     8641 taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata
     8701 acgttcttaa tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc
     8761 taactccttc cttttcggtt agagcggatc ttagctagcc gcggtaccaa gcttactcga
     8821 gctacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     8881 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg tagctcaggt
     8941 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     9001 cggcgagctg cacgctgccg tcctcgatgt tgtggcgga
//
