LOCUS       sequence-237533-       10141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast-targeted expression of mCitrine controlled by the CaMV 
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CTP-mCitrine
FEATURES             Location/Qualifiers
     source          1..10141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(610..612,613..1326)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEMFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(652..673)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(913..932)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1260..1281
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1329..1353)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1673..1697
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3028..3657
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4086..5159
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5225..5419
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5655..5677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5763..5903
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5818..5837
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5918..5935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6089..6677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6169..6188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6923..7714)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8235..8259
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8352..8604)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8627..9199)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9241..9420)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggct tcctctatgc tctcttccgc tactatggtt
      481 gcctctccgg ctcaggccac tatggtcgct cctttcaacg gacttaagtc ctccgctgcc
      541 ttcccagcca cccgcaaggc taacaacgac attacttcca tcacaagcaa cggcggaaga
      601 gttaactgcg tgagcaaggg cgaggagatg ttcaccgggg tggtgcccat cctggtcgag
      661 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      781 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
      841 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      901 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      961 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1021 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1081 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1141 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1201 aaccactacc tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     1261 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1321 aagtaatcgt tcagctttct tgtacaaagt ggtgatatcc cgcggccatg ctagagtccg
     1381 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
     1441 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
     1501 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     1561 aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgggccc aagcttagct
     1621 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     1681 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     1741 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     1801 ctcgggatca aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc
     1861 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     1921 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     1981 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     2041 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     2101 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     2161 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     2221 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     2281 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     2341 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     2401 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     2461 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     2521 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     2581 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     2641 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     2701 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     2761 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     2821 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     2881 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     2941 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     3001 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     3061 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     3121 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     3181 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     3241 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     3301 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     3361 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     3421 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     3481 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     3541 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     3601 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     3661 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     3721 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     3781 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     3841 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     3901 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     3961 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     4021 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     4081 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     4141 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     4201 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     4261 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     4321 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     4381 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     4441 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     4501 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     4561 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     4621 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     4681 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     4741 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     4801 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     4861 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     4921 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     4981 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     5041 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     5101 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     5161 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     5221 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     5281 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     5341 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     5401 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     5461 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     5521 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     5581 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     5641 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5701 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5761 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     5821 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5881 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6721 acgaaaactc acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg
     6781 cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc
     6841 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
     6901 tgaacgaatt tctagctaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     6961 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     7021 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     7081 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     7141 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     7201 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     7261 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     7321 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     7381 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     7441 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     7501 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     7561 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     7621 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     7681 gagtcgatac ttcggcgatc accgcttccc ccatgatgtt taactttgtt ttagggcgac
     7741 tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     7801 gcgcttgctg cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag
     7861 ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt
     7921 tgcgtgagcg catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact
     7981 gggttcgtgc ccgaattgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     8041 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     8101 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     8161 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     8221 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     8281 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattgaa ttatcagctt
     8341 gcatgccggt cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     8401 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     8461 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     8521 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     8581 ttattgccaa atgtttgaac gatctgcttg actctagggg tcatcattag gggcagggca
     8641 tgctcatgta gagcgcctgc tcgccgtccg aggcggtgcc gtcgtacagg gcggtgtcca
     8701 ggccgcagag ggtgaacccc atccgccggt acgcgtggat cgccggtgcg ttgacgttgg
     8761 tgacctccag ccagaggtgc ccggcacccc gctcgcgggc gaactccgtc gcgagcccca
     8821 tcaacgcgcg cccgaccccg tgcccccggt gctccggggc gacctcgatg tcctcgacgg
     8881 tcagccggcg gttccagccg gagtacgaga cgaccacgaa gcccgccagg tcgccgtcgt
     8941 ccccgtacgc gacgaacgtc cgagagtccg ggtcgccgtc ctccccgtcg tccgattcgt
     9001 cgtccgattc gtcgtcgggg aacaccttgg tcaggggcgg gtccaccggc acctcccgca
     9061 gggtgaagcc gtccccggtg gcggtgacgc ggaagacggt gtcggtggtg aaggacccat
     9121 ccagtgcctc gatggcctcg gcgtcccccg ggacactggt gcggtaccgg taagccgtgt
     9181 cgtcaagagt ggtacccatg gtagatcccc tcgatcgagt tgagagtgaa tatgagactc
     9241 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
     9301 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
     9361 tgcttagctc attaaactcc agaaacccgc ggctcagtgg ctccttcaac gttgcggttc
     9421 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
     9481 cccttaattc tccgctcatg atcgataatt cgagctctcc catatggtcg actagagcca
     9541 agctgatctc ctttgccccg gagatcacca tggacgactt tctctatctc tacgatctag
     9601 gaagaaagtt cgacggagaa ggtgacgata ccatgttcac caccgataat gagaagatta
     9661 gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc tacgcggcag
     9721 gtctcatcaa gacgatctac ccgagtaata atctccagga gatcaaatac cttcccaaga
     9781 aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata
     9841 tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg cttcataaac
     9901 caaggcaagt aatagagatt ggagtctcta agaaagtagt tcctactgaa tcaaaggcca
     9961 tggagtcaaa aattcagatc gaggatctaa cagaactcgc cgtgaagact ggcgaacagt
    10021 tcatacagag tcttttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc
    10081 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac caaagggcta
    10141 t
//
