LOCUS       sequence-237572-         638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-103a-2-5p target in 3' UTR of of mKate. miRNA activity can 
            be measured by the repression of mKate2 relative to EBFP2 
            fluorescent proteins . Plasmid is inherently unstable; please see 
            Depositor Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-103a-2-5p
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(112..136)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(364..383)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    429..484
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(483..502)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gaatcaagga ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat
       61 actgcgacct ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt
      121 tcttgtacaa agtggtacgt ctcaagacca gaggtctctg cttcgtcgtg gcaaggcagc
      181 actgtaaaga agctcaaggc agcactgtaa agaagctcaa ggcagcactg taaagaagct
      241 caaggcagca ctgtaaagaa gctcaaccag aagagacctg tgcttcactc ctcaggtgca
      301 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      361 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      421 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
      481 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
      541 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
      601 aggtcatcag tatatgaaac agccccctgc tgtccatt
//
