LOCUS       sequence-237609-         935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-105-3p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-105-3p
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(109..133)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(357..376)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    422..477
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(476..495)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    861..886
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 tcaaggaggc cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact
       61 gcgacctccc tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct
      121 tgtacaaagt ggtacgtctc aagaccagag gtctctgctt actgctttta gcacatgctc
      181 aaacatccgt tagcacatgc tcaaacatcc gttagcacat gctcaaacat ccgttagcac
      241 atgctcaaac atccgtcaac cagaagagac ctgtgcttca ctcctcaggt gcaggctgcc
      301 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
      361 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
      421 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
      481 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
      541 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
      601 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
      661 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
      721 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
      781 gtccctcttc tcttatggag atcccgagcg gtgcgttttt atgcttgtag tattgtataa
      841 tgtttttaag atccaagctt ctgacctctt ctcttcctcc cacagggcct cgagacaatt
      901 gcatcgatgg taccagtgaa agtctgcaat gaatt
//
