LOCUS       sequence-237629-         599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-125b-5p
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(98..122)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(346..365)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    411..466
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(465..484)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gacaaagaga catacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
       61 agcaaactgg ggcacaaact taattgagaa ttctaccacc cagctttctt gtacaaagtg
      121 gtacgtctca agaccagagg tctctgcttt gtattgttca caagttagcg tctcagggat
      181 cacaagttag cgtctcaggg atcacaagtt agcgtctcag ggatcacaag ttagcgtctc
      241 agggacaacc agaagagacc tgtgcttcac tcctcaggtg caggctgcct atcagaaggt
      301 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
      361 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
      421 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
      481 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
      541 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatga
//
