LOCUS       sequence-237683-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AKT1 E17K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AKT1-E17K
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..1204
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          257..1195
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1152..1172
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1224..1248
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1251..2690
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="MSDVAIVKEGWLHKRGKYIKTWRPRYFLLKNDGTFIGYKERPQDV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK
                     VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR
                     LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLM
                     LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY
                     EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA
                     KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM
                     ECVDSERRPHFPQFSYSASGTA"
     protein_bind    complement(2695..2719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2772..2813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2834..3401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2851..3403
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2995..3012)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3222..3241
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3415..3417,3418..3420,3421..4134)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3415..3417,3418..3420,3421..4131)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3460..3481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3721..3740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4068..4089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4148..4736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4201..4221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4619..4630)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4811..5044
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5163..5181)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5249..5353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5354..5422,5423..6214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5572..5591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6385..6973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6874..6893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7127..7144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7384..7405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7420..7425,7426..7443,7444..7450)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7458..7474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7463..7485
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7482..7498
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7482..7498
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7548..7567
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7759..8392
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8439..8564
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9061..9294
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9479..9523
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9816..9933
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagatccagt ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
       61 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      121 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      181 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      241 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      301 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      361 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      421 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      481 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      541 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      601 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      661 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      721 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      781 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      841 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      901 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
      961 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1021 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1081 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1141 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1201 gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa agcaggcacc atgagcgacg
     1261 tggctattgt gaaggagggt tggctgcaca aacgagggaa gtacatcaag acctggcggc
     1321 cacgctactt cctcctcaag aatgatggca ccttcattgg ctacaaggag cggccgcagg
     1381 atgtggacca acgtgaggct cccctcaaca acttctctgt ggcgcagtgc cagctgatga
     1441 agacggagcg gccccggccc aacaccttca tcatccgctg cctgcagtgg accactgtca
     1501 tcgaacgcac cttccatgtg gagactcctg aggagcggga ggagtggaca accgccatcc
     1561 agactgtggc tgacggcctc aagaagcagg aggaggagga gatggacttc cggtcgggct
     1621 cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag cccaagcacc
     1681 gcgtgaccat gaacgagttt gagtacctga agctgctggg caagggcact ttcggcaagg
     1741 tgatcctggt gaaggagaag gccacaggcc gctactacgc catgaagatc ctcaagaagg
     1801 aagtcatcgt ggccaaggac gaggtggccc acacactcac cgagaaccgc gtcctgcaga
     1861 actccaggca ccccttcctc acagccctga agtactcttt ccagacccac gaccgcctct
     1921 gctttgtcat ggagtacgcc aacgggggcg agctgttctt ccacctgtcc cgggagcgtg
     1981 tgttctccga ggaccgggcc cgcttctatg gcgctgagat tgtgtcagcc ctggactacc
     2041 tgcactcgga gaagaacgtg gtgtaccggg acctcaagct ggagaacctc atgctggaca
     2101 aggacgggca cattaagatc acagacttcg ggctgtgcaa ggaggggatc aaggacggtg
     2161 ccaccatgaa gaccttttgc ggcacacctg agtacctggc ccccgaggtg ctggaggaca
     2221 atgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac gagatgatgt
     2281 gcggtcgcct gcccttctac aaccaggacc atgagaagct ttttgagctc atcctcatgg
     2341 aggagatccg cttcccgcgc acgcttggtc ccgaggccaa gtccttgctt tcagggctgc
     2401 tcaagaagga ccccaagcag aggcttggcg ggggctccga ggacgccaag gagatcatgc
     2461 agcatcgctt ctttgccggt atcgtgtggc agcacgtgta cgagaagaag ctcagcccac
     2521 ccttcaagcc ccaggtcacg tcggagactg acaccaggta ttttgatgag gagttcacgg
     2581 cccagatgat caccatcaca ccacctgacc aagatgacag catggagtgt gtggacagcg
     2641 agcgcaggcc ccacttcccc cagttctcct actcggccag cggcacggcc tagaacccag
     2701 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     2761 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     2821 gttagtaatg agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     2881 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     2941 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3001 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     3061 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     3121 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     3181 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     3241 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     3301 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     3361 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatggtg
     3421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4021 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaatcgat
     4141 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     4201 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     4261 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     4321 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     4381 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     4441 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4501 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4561 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4621 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4681 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     4741 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4801 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     4861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     4921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     4981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5041 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5101 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     5161 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5221 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5281 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5341 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5401 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5461 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5521 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5581 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     5641 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5701 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5761 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5821 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5881 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5941 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6001 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6061 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6121 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6181 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6241 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6301 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6361 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6421 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6481 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6541 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6601 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6661 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6721 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6781 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6841 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6901 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6961 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7021 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7081 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7141 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7201 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7261 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7321 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7381 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7441 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     7501 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7561 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     7621 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     7681 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     7741 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     7801 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     7861 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     7921 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     7981 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     8041 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     8101 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     8161 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     8221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8401 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8461 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8521 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8581 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8641 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8701 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8761 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8821 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8881 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     8941 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9001 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9061 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9121 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9181 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9241 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9301 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     9361 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9421 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9481 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9541 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9601 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9661 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     9721 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     9781 tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     9841 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     9901 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagca
//
