LOCUS       sequence-237684-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF T241P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-T241P
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..1197
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          250..1188
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1145..1165
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1217..1241
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1242..1251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3546..3570)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3578..3596
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3621..3640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3622..3640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3646..3666)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3720..3761
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3782..4349
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3799..4351
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3943..3960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4170..4189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4363..4962
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4363..4382)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4859..4879
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4976..5564
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5029..5049)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5447..5458)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5639..5872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5991..6009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6077..6181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6182..6250,6251..7042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6400..6419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7213..7801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7702..7721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7955..7972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8212..8233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8248..8253,8254..8271,8272..8278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8286..8302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8291..8313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8310..8326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8310..8326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8376..8395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8587..9220
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9267..9392
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9889..10122
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10307..10351
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10644..10761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
       61 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      121 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      181 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      241 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      301 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
      361 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
      421 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
      481 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
      541 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
      601 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
      661 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
      721 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
      781 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
      841 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
      901 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
      961 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     1021 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     1081 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     1141 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc
     1201 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatggcgg cgctgagcgg
     1261 tggcggtggt ggcggcgcgg agccgggcca ggctctgttc aacggggaca tggagcccga
     1321 ggccggcgcc ggcgccggcg ccgcggcctc ttcggctgcg gaccctgcca ttccggagga
     1381 ggtgtggaat atcaaacaaa tgattaagtt gacacaggaa catatagagg ccctattgga
     1441 caaatttggt ggggagcata atccaccatc aatatatctg gaggcctatg aagaatacac
     1501 cagcaagcta gatgcactcc aacaaagaga acaacagtta ttggaatctc tggggaacgg
     1561 aactgatttt tctgtttcta gctctgcatc aatggatacc gttacatctt cttcctcttc
     1621 tagcctttca gtgctacctt catctctttc agtttttcaa aatcccacag atgtggcacg
     1681 gagcaacccc aagtcaccac aaaaacctat cgttagagtc ttcctgccca acaaacagag
     1741 gacagtggta cctgcaaggt gtggagttac agtccgagac agtctaaaga aagcactgat
     1801 gatgagaggt ctaatcccag agtgctgtgc tgtttacaga attcaggatg gagagaagaa
     1861 accaattggt tgggacactg atatttcctg gcttactgga gaagaattgc atgtggaagt
     1921 gttggagaat gttccactta caacacacaa ctttgtacga aaaccgtttt tcaccttagc
     1981 attttgtgac ttttgtcgaa agctgctttt ccagggtttc cgctgtcaaa catgtggtta
     2041 taaatttcac cagcgttgta gtacagaagt tccactgatg tgtgttaatt atgaccaact
     2101 tgatttgctg tttgtctcca agttctttga acaccaccca ataccacagg aagaggcgtc
     2161 cttagcagag actgccctaa catctggatc atccccttcc gcacccgcct cggactctat
     2221 tgggccccaa attctcacca gtccgtctcc ttcaaaatcc attccaattc cacagccctt
     2281 ccgaccagca gatgaagatc atcgaaatca atttgggcaa cgagaccgat cctcatcagc
     2341 tcccaatgtg catataaaca caatagaacc tgtcaatatt gatgacttga ttagagacca
     2401 aggatttcgt ggtgatggag gatcaaccac aggtttgtct gctacccccc ctgcctcatt
     2461 acctggctca ctaactaacg tgaaagcctt acagaaatct ccaggacctc agcgagaaag
     2521 gaagtcatct tcatcctcag aagacaggaa tcgaatgaaa acacttggta gacgggactc
     2581 gagtgatgat tgggagattc ctgatgggca gattacagtg ggacaaagaa ttggatctgg
     2641 atcatttgga acagtctaca agggaaagtg gcatggtgat gtggcagtga aaatgttgaa
     2701 tgtgacagca cctacacctc agcagttaca agccttcaaa aatgaagtag gagtactcag
     2761 gaaaacacga catgtgaata tcctactctt catgggctat tccacaaagc cacaactggc
     2821 tattgttacc cagtggtgtg agggctccag cttgtatcac catctccata tcattgagac
     2881 caaatttgag atgatcaaac ttatagatat tgcacgacag actgcacagg gcatggatta
     2941 cttacacgcc aagtcaatca tccacagaga cctcaagagt aataatatat ttcttcatga
     3001 agacctcaca gtaaaaatag gtgattttgg tctagctaca gtgaaatctc gatggagtgg
     3061 gtcccatcag tttgaacagt tgtctggatc cattttgtgg atggcaccag aagtcatcag
     3121 aatgcaagat aaaaatccat acagctttca gtcagatgta tatgcatttg gaattgttct
     3181 gtatgaattg atgactggac agttacctta ttcaaacatc aacaacaggg accagataat
     3241 ttttatggtg ggacgaggat acctgtctcc agatctcagt aaggtacgga gtaactgtcc
     3301 aaaagccatg aagagattaa tggcagagtg cctcaaaaag aaaagagatg agagaccact
     3361 ctttccccaa attctcgcct ctattgagct gctggcccgc tcattgccaa aaattcaccg
     3421 cagtgcatca gaaccctcct tgaatcgggc tggtttccaa acagaggatt ttagtctata
     3481 tgcttgtgct tctccaaaaa cacccatcca ggcaggggga tatggtgcgt ttcctgtcca
     3541 ctagaaccca gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggttta
     3601 agtgagttat agaagtagta ccctatagtg agtcgtatta ccacccaact tttctataca
     3661 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
     3721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag
     3781 atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     3841 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3901 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3961 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4021 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4081 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4141 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4201 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4261 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     4321 tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc
     4381 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4441 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4501 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4561 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4621 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4681 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4741 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4801 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     4861 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     4921 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca
     4981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     5041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     5101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     5161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     5221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     5281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     5341 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     5401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     5461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     5521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     5581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5641 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     5701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     5881 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     5941 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     6001 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6061 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6421 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     8041 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8101 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     8161 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8221 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8281 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     8341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8401 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     8461 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     8521 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     8581 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     8641 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     8701 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     8761 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     8821 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     8881 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     8941 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     9001 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     9061 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9121 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9181 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     9241 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     9301 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     9361 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     9421 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     9481 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     9541 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     9601 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     9661 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     9721 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9781 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9841 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9901 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9961 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    10021 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    10081 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    10141 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    10201 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    10261 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    10321 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    10381 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10441 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10501 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    10561 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
    10621 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
    10681 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
    10741 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
//
