LOCUS       sequence-237698-       12257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB2 V842I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-V842I
FEATURES             Location/Qualifiers
     source          1..12257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    158..275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        323..1501
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          554..1492
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1449..1469
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1521..1545
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5317..5341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5349..5367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5392..5411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5393..5411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5417..5437)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5491..5532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5553..6120
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5570..6122
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5714..5731)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5941..5960
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6134..6733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6134..6153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6630..6650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6747..7335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6800..6820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7218..7229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7410..7643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7762..7780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7848..7952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7953..8021,8022..8813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8171..8190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8984..9572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9473..9492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9726..9743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9983..10004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10019..10024,10025..10042,10043..10049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10057..10073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10062..10084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10081..10097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10081..10097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10147..10166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10358..10991
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11038..11163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11660..11893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12078..12122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
       61 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      121 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      181 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      241 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      301 atccagtttg gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      361 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      421 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      481 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      541 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      601 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      661 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      721 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      781 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      841 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
      901 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
      961 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1021 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1081 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1141 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1201 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1261 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1321 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1381 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1441 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1501 aagcggccct gcagatatca acaagtttgt acaaaaaagc aggcaccatg gagctggcgg
     1561 ccttgtgccg ctgggggctc ctcctcgccc tcttgccccc cggagccgcg agcacccaag
     1621 tgtgcaccgg cacagacatg aagctgcggc tccctgccag tcccgagacc cacctggaca
     1681 tgctccgcca cctctaccag ggctgccagg tggtgcaggg aaacctggaa ctcacctacc
     1741 tgcccaccaa tgccagcctg tccttcctgc aggatatcca ggaggtgcag ggctacgtgc
     1801 tcatcgctca caaccaagtg aggcaggtcc cactgcagag gctgcggatt gtgcgaggca
     1861 cccagctctt tgaggacaac tatgccctgg ccgtgctaga caatggagac ccgctgaaca
     1921 ataccacccc tgtcacaggg gcctccccag gaggcctgcg ggagctgcag cttcgaagcc
     1981 tcacagagat cttgaaagga ggggtcttga tccagcggaa cccccagctc tgctaccagg
     2041 acacgatttt gtggaaggac atcttccaca agaacaacca gctggctctc acactgatag
     2101 acaccaaccg ctctcgggcc tgccacccct gttctccgat gtgtaagggc tcccgctgct
     2161 ggggagagag ttctgaggat tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg
     2221 cccgctgcaa ggggccactg cccactgact gctgccatga gcagtgtgct gccggctgca
     2281 cgggccccaa gcactctgac tgcctggcct gcctccactt caaccacagt ggcatctgtg
     2341 agctgcactg cccagccctg gtcacctaca acacagacac gtttgagtcc atgcccaatc
     2401 ccgagggccg gtatacattc ggcgccagct gtgtgactgc ctgtccctac aactaccttt
     2461 ctacggacgt gggatcctgc accctcgtct gccccctgca caaccaagag gtgacagcag
     2521 aggatggaac acagcggtgt gagaagtgca gcaagccctg tgcccgagtg tgctatggtc
     2581 tgggcatgga gcacttgcga gaggtgaggg cagttaccag tgccaatatc caggagtttg
     2641 ctggctgcaa gaagatcttt gggagcctgg catttctgcc ggagagcttt gatggggacc
     2701 cagcctccaa cactgccccg ctccagccag agcagctcca agtgtttgag actctggaag
     2761 agatcacagg ttacctatac atctcagcat ggccggacag cctgcctgac ctcagcgtct
     2821 tccagaacct gcaagtaatc cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc
     2881 tgcaagggct gggcatcagc tggctggggc tgcgctcact gagggaactg ggcagtggac
     2941 tggccctcat ccaccataac acccacctct gcttcgtgca cacggtgccc tgggaccagc
     3001 tctttcggaa cccgcaccaa gctctgctcc acactgccaa ccggccagag gacgagtgtg
     3061 tgggcgaggg cctggcctgc caccagctgt gcgcccgagg gcactgctgg ggtccagggc
     3121 ccacccagtg tgtcaactgc agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc
     3181 gagtactgca ggggctcccc agggagtatg tgaatgccag gcactgtttg ccgtgccacc
     3241 ctgagtgtca gccccagaat ggctcagtga cctgttttgg accggaggct gaccagtgtg
     3301 tggcctgtgc ccactataag gaccctccct tctgcgtggc ccgctgcccc agcggtgtga
     3361 aacctgacct ctcctacatg cccatctgga agtttccaga tgaggagggc gcatgccagc
     3421 cttgccccat caactgcacc cactcctgtg tggacctgga tgacaagggc tgccccgccg
     3481 agcagagagc cagccctctg acgtccatca tctctgcggt ggttggcatt ctgctggtcg
     3541 tggtcttggg ggtggtcttt gggatcctca tcaagcgacg gcagcagaag atccggaagt
     3601 acacgatgcg gagactgctg caggaaacgg agctggtgga gccgctgaca cctagcggag
     3661 cgatgcccaa ccaggcgcag atgcggatcc tgaaagagac ggagctgagg aaggtgaagg
     3721 tgcttggatc tggcgctttt ggcacagtct acaagggcat ctggatccct gatggggaga
     3781 atgtgaaaat tccagtggcc atcaaagtgt tgagggaaaa cacatccccc aaagccaaca
     3841 aagaaatctt agacgaagca tacgtgatgg ctggtgtggg ctccccatat gtctcccgcc
     3901 ttctgggcat ctgcctgaca tccacggtgc agctggtgac acagcttatg ccctatggct
     3961 gcctcttaga ccatgtccgg gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact
     4021 ggtgtatgca gattgccaag gggatgagct acctggagga tgtgcggctc atacacaggg
     4081 acttggccgc tcggaacgtg ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg
     4141 ggctggctcg gctgctggac attgacgaga cagagtacca tgcagatggg ggcaaggtgc
     4201 ccatcaagtg gatggcgctg gagtccattc tccgccggcg gttcacccac cagagtgatg
     4261 tgtggagtta tggtgtgact gtgtgggagc tgatgacttt tggggccaaa ccttacgatg
     4321 ggatcccagc ccgggagatc cctgacctgc tggaaaaggg ggagcggctg ccccagcccc
     4381 ccatctgcac cattgatgtc tacatgatca tggtcaaatg ttggatgatt gactctgaat
     4441 gtcggccaag attccgggag ttggtgtctg aattctcccg catggccagg gacccccagc
     4501 gctttgtggt catccagaat gaggacttgg gcccagccag tcccttggac agcaccttct
     4561 accgctcact gctggaggac gatgacatgg gggacctggt ggatgctgag gagtatctgg
     4621 taccccagca gggcttcttc tgtccagacc ctgccccggg cgctgggggc atggtccacc
     4681 acaggcaccg cagctcatct accaggagtg gcggtgggga cctgacacta gggctggagc
     4741 cctctgaaga ggaggccccc aggtctccac tggcaccctc cgaaggggct ggctccgatg
     4801 tatttgatgg tgacctggga atgggggcag ccaaggggct gcaaagcctc cccacacatg
     4861 accccagccc tctacagcgg tacagtgagg accccacagt acccctgccc tctgagactg
     4921 atggctacgt tgcccccctg acctgcagcc cccagcctga atatgtgaac cagccagatg
     4981 ttcggcccca gcccccttcg ccccgagagg gccctctgcc tgctgcccga cctgctggtg
     5041 ccactctgga aaggcccaag actctctccc cagggaagaa tggggtcgtc aaagacgttt
     5101 ttgcctttgg gggtgccgtg gagaaccccg agtacttgac accccaggga ggagctgccc
     5161 ctcagcccca ccctcctcct gccttcagcc cagccttcga caacctctat tactgggacc
     5221 aggacccacc agagcggggg gctccaccca gcaccttcaa agggacacct acggcagaga
     5281 acccagagta cctgggtctg gacgtgccag tgtagaaccc agctttcttg tacaaagtgg
     5341 tgatatccaa ttaaccctca ctaaaggtat aagaagtaat ttgagaagag tccctatagt
     5401 gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca cagtggcggc
     5461 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5521 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc
     5581 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5641 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5701 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5761 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5821 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5881 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5941 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6001 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6061 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6121 tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6721 gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     6781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6901 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6961 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7021 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7081 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7141 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7201 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7261 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7321 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     7381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7441 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7621 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7681 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     7741 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8161 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     8221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9781 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     9841 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9901 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     9961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10081 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    10141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10201 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10261 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10321 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10381 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10441 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10501 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10561 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10621 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10681 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10741 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10801 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10861 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10921 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10981 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11041 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11101 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11161 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11221 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11281 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11341 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11401 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11461 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
    11521 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    11581 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    11641 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    11701 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    11761 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    11821 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    11881 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    11941 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    12001 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    12061 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    12121 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
    12181 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
    12241 ggcagggata ttcacca
//
