LOCUS       sequence-237707-        9694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MAP2K1 S222A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-MAP2K1-S222A
FEATURES             Location/Qualifiers
     source          1..9694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    102..219
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        267..1445
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          498..1436
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1393..1413
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1465..1489
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2675..2699)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2752..2793
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2814..3381
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2831..3383
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2975..2992)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3202..3221
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3395..3397,3398..3400,3401..4114)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3395..3397,3398..3400,3401..4111)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3440..3461)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3701..3720)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4048..4069
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4128..4716
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4181..4201)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4599..4610)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4791..5024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5143..5161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5229..5333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5334..5402,5403..6194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5552..5571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6365..6953
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6854..6873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7107..7124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7364..7385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7400..7405,7406..7423,7424..7430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7438..7454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7443..7465
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7462..7478
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7462..7478
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7528..7547
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7739..8372
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8419..8544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9041..9274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9459..9503
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
       61 acggtatcgc cgaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg
      121 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
      181 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
      241 agagatccag tttggactag tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat
      301 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
      361 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      421 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      481 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      541 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
      601 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
      661 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
      721 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
      781 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
      841 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
      901 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
      961 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     1021 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     1081 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     1141 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     1201 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     1261 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     1321 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     1381 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     1441 cgtgaagcgg ccctgcagat atcaacaagt ttgtacaaaa aagcaggcac catgcccaag
     1501 aagaagccga cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc
     1561 agctctgcgg agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat
     1621 gagcagcagc gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg
     1681 aaggatgacg actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc
     1741 aaggtctccc acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc
     1801 aaacccgcaa tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct
     1861 ccgtacatcg tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg
     1921 gagcacatgg atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa
     1981 caaattttag gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggagaag
     2041 cacaagatca tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag
     2101 atcaagctct gtgactttgg ggtcagcggg cagctcatcg actccatggc caacgccttc
     2161 gtgggcacaa ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag
     2221 tcagacatct ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc
     2281 cctcctccag atgccaagga gctggagctg atgtttgggt gccaggtgga aggagatgcg
     2341 gctgagaccc cacccaggcc aaggaccccc gggaggcccc ttagctcata cggaatggac
     2401 agccgacctc ccatggcaat ttttgagttg ttggattaca tagtcaacga gcctcctcca
     2461 aaactgccca gtggagtgtt cagtctggaa tttcaagatt ttgtgaataa atgcttaata
     2521 aaaaaccccg cagagagagc agatttgaag caactcatgg ttcatgcttt tatcaagaga
     2581 tctgatgctg aggaagtgga ttttgcaggt tggctctgct ccaccatcgg ccttaaccag
     2641 cccagcacac caacccatgc tgctggcgtc tagaacccag ctttcttgta caaagtggtt
     2701 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     2761 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agatccctcc
     2821 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2881 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2941 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3001 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3061 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3121 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3181 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3241 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3301 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3361 ctttgaaaaa cacgatgata atatggccac acatatggtg agcaagggcg aggagctgtt
     3421 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3481 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3541 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3601 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3661 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3721 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3781 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3841 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3901 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3961 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4021 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4081 gatcactctc ggcatggacg agctgtacaa gtaaatcgat agatcctaat caacctctgg
     4141 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4201 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4261 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4321 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4381 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4441 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4501 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4561 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4621 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4681 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     4741 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4801 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5041 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     5101 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     5161 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     5221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5521 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     5581 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5641 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5701 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5761 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5821 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5881 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5941 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6001 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6061 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6121 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6181 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6241 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6361 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6421 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6481 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6541 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6601 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6661 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6721 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7021 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7081 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7141 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     7201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     7321 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7381 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7441 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     7501 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7561 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     7621 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     7681 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     7741 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     7801 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     7861 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     7921 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7981 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     8041 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     8101 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     8161 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     8221 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8281 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8341 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8401 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8461 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8521 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8581 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8641 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8701 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8761 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8821 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8881 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8941 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     9001 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     9061 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     9121 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     9181 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     9241 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     9301 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     9361 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     9421 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     9481 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     9541 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     9601 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9661 caaccccgag gggacccgac aggcccgaag gaat
//
