LOCUS       sequence-237729-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_BR29_00096171
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(459..475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(459..475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(472..494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    483..499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(507..513,514..531,532..537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    552..573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(690..707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcatctc aaagcacaag tagtaccaaa tcttttacca tatggaattt
       61 caataatatg atccattttt ggtctagttt ttggatctct tctataacct tcagatctat
      121 atggtttacc agcaatagaa atagtaaccc atggatcaga tttaaaagtc ataatttgcc
      181 atcttcttgg aaaagctttt tgaccagaag acaattcagc agcacaattt tttggttgag
      241 ctttatcaac atgagtagta aaagtccatt gttgttcagt ttcagtacca gtatcaattc
      301 taacaatagc ttgttgacca gtaacagctt taccataacc attcataaca ccatcagaat
      361 ctttaaccca aatatgacaa tcagaatcag tagtttcaaa atcaaccatt agagaccaag
      421 ttccatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt tcctgtgtga
      481 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      541 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      601 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaa
//
