LOCUS       sequence-237745-       10921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR4
FEATURES             Location/Qualifiers
     source          1..10921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        309..1487
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          540..1478
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1435..1455
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1507..1531
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2702..2713
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3944..3968)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4021..4062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4083..4650
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4100..4652
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4244..4261)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4471..4490
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4664..4666,4667..4669,4670..5383)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4664..4666,4667..4669,4670..5380)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4709..4730)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4970..4989)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5317..5338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5397..5985
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5450..5470)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5868..5879)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6060..6293
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6412..6430)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6498..6602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6603..6671,6672..7463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6821..6840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7634..8222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8123..8142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8376..8393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8633..8654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8669..8674,8675..8692,8693..8699)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8707..8723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8712..8734
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8731..8747
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8731..8747
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8797..8816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9008..9641
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9688..9813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10310..10543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10728..10772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
       61 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca
      121 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      181 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      241 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact
      301 agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      361 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      421 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      481 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      541 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      601 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      661 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      721 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      781 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      841 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      901 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      961 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     1021 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     1081 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     1141 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1201 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1261 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1321 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1381 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1441 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc ggccctgcag
     1501 atatcaacaa gtttgtacaa aaaagcaggc accatgcggc tgctgctggc cctgttgggg
     1561 gtcctgctga gtgtgcctgg gcctccagtc ttgtccctgg aggcctctga ggaagtggag
     1621 cttgagccct gcctggctcc cagcctggag cagcaagagc aggagctgac agtagccctt
     1681 gggcagcctg tgcgtctgtg ctgtgggcgg gctgagcgtg gtggccactg gtacaaggag
     1741 ggcagtcgcc tggcacctgc tggccgtgta cggggctgga ggggccgcct agagattgcc
     1801 agcttcctac ctgaggatgc tggccgctac ctctgcctgg cacgaggctc catgatcgtc
     1861 ctgcagaatc tcaccttgat tacaggtgac tccttgacct ccagcaacga tgatgaggac
     1921 cccaagtccc atagggaccc ctcgaatagg cacagttacc cccagcaagc accctactgg
     1981 acacaccccc agcgcatgga gaagaaactg catgcagtac ctgcggggaa caccgtcaag
     2041 ttccgctgtc cagctgcagg caaccccacg cccaccatcc gctggcttaa ggatggacag
     2101 gcctttcatg gggagaaccg cattggaggc attcggctgc gccatcagca ctggagtctc
     2161 gtgatggaga gcgtggtgcc ctcggaccgc ggcacataca cctgcctggt agagaacgct
     2221 gtgggcagca tccgctataa ctacctgcta gatgtgctgg agcggtcccc gcaccggccc
     2281 atcctgcagg ccgggctccc ggccaacacc acagccgtgg tgggcagcga cgtggagctg
     2341 ctgtgcaagg tgtacagcga tgcccagccc cacatccagt ggctgaagca catcgtcatc
     2401 aacggcagca gcttcggagc cgacggtttc ccctatgtgc aagtcctaaa gactgcagac
     2461 atcaatagct cagaggtgga ggtcctgtac ctgcggaacg tgtcagccga ggacgcaggc
     2521 gagtacacct gcctcgcagg caattccatc ggcctctcct accagtctgc ctggctcacg
     2581 gtgctgccag aggaggaccc cacatggacc gcagcagcgc ccgaggccag gtatacggac
     2641 atcatcctgt acgcgtcggg ctccctggcc ttggctgtgc tcctgctgct ggccgggctg
     2701 tatcgagggc aggcgctcca cggccggcac ccccgcccgc ccgccactgt gcagaagctc
     2761 tcccgcttcc ctctggcccg acagttctcc ctggagtcag gctcttccgg caagtcaagc
     2821 tcatccctgg tacgaggcgt gcgtctctcc tccagcggcc ccgccttgct cgccggcctc
     2881 gtgagtctag atctacctct cgacccacta tgggagttcc cccgggacag gctggtgctt
     2941 gggaagcccc taggcgaggg ctgctttggc caggtagtac gtgcagaggc ctttggcatg
     3001 gaccctgccc ggcctgacca agccagcact gtggccgtca agatgctcaa agacaacgcc
     3061 tctgacaagg acctggccga cctggtctcg gagatggagg tgatgaagct gatcggccga
     3121 cacaagaaca tcatcaacct gcttggtgtc tgcacccagg aagggcccct gtacgtgatc
     3181 gtggagtgcg ccgccaaggg aaacctgcgg gagttcctgc gggcccggcg ccccccaggc
     3241 cccgacctca gccccgacgg tcctcggagc agtgaggggc cgctctcctt cccagtcctg
     3301 gtctcctgcg cctaccaggt ggcccgaggc atgcagtatc tggagtcccg gaagtgtatc
     3361 caccgggacc tggctgcccg caatgtgctg gtgactgagg acaatgtgat gaagattgct
     3421 gactttgggc tggcccgcgg cgtccaccac attgactact ataagaaaac cagcaacggc
     3481 cgcctgcctg tgaagtggat ggcgcccgag gccttgtttg accgggtgta cacacaccag
     3541 agtgacgtgt ggtcttttgg gatcctgcta tgggagatct tcaccctcgg gggctccccg
     3601 tatcctggca tcccggtgga ggagctgttc tcgctgctgc gggagggaca tcggatggac
     3661 cgacccccac actgcccccc agagctgtac gggctgatgc gtgagtgctg gcacgcagcg
     3721 ccctcccaga ggcctacctt caagcagctg gtggaggcgc tggacaaggt cctgctggcc
     3781 gtctctgagg agtacctcga cctccgcctg accttcggac cctattcccc ctctggtggg
     3841 gacgccagca gcacctgctc ctccagcgat tctgtcttca gccacgaccc cctgccattg
     3901 ggatccagct ccttcccctt cgggtctggg gtgcagacat agaacccagc tttcttgtac
     3961 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     4021 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     4081 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4141 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4201 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4261 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4321 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4381 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4441 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4501 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4561 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4621 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatggtga gcaagggcga
     4681 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4741 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4801 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4861 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4921 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4981 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     5041 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     5101 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     5161 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     5221 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     5281 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     5341 cgccgccggg atcactctcg gcatggacga gctgtacaag taaatcgata gatcctaatc
     5401 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5461 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5521 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5581 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5641 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5701 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5761 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     5821 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5881 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5941 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     6001 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6061 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6121 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6181 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6241 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6301 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6361 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     6421 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6841 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8401 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     8461 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8521 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8581 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     8641 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     8701 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     8761 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8821 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     8881 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     8941 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9001 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     9061 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     9121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     9181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     9241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     9301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     9361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
     9421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
     9481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     9661 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     9721 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     9781 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     9841 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     9901 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     9961 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10021 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10081 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10141 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    10441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    10501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    10561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    10621 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    10681 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    10741 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    10801 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10861 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10921 c
//
