LOCUS       sequence-237879-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28B-PU.1-ETS-Wing
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             369..386
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             393..410
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             435..452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(505..523)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      519..566
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      603..1058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(690..709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     900..921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tnnnnnctnn anaattttgt ttaactttaa gaaggagata taccatgggt tctaagaaga
       61 agatccgctt ataccagttc ttattggact tattacgctc gggcgatatg aaggattcca
      121 tttggtgggt ggataaggat aaggggacgt ttcagttctc aagtaaacac aaagaggcgc
      181 tggcacatcg ctggggcatt caaaaaggaa atcgcaagaa gatgacctat cagaaaatgg
      241 ctcgtgccct gcgtaactac gggaagacag gtgaggtgaa aaaggtcgca gggaagcgct
      301 taacttatca gttcagtggt gaagtattag gccgtggggg cttagcggaa cgtcgcttac
      361 cacctcactt agttccccgc ggatctcgta gtcatcacca ccatcatcac taaaagcttg
      421 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      481 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      541 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      601 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1021 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     1081 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1141 aattaattct tanaaaaact catcgagcat caantgaaac tgccattttt nnnnntccgg
     1201 attancanta ccnnnttttn gaaaaaaccn tnnntgtaan gnngnaaaaa annccccnag
     1261 gcnnttcctn nggnnggcng aatcnnggna ncgggctggc aatccnancc cccnaaannn
     1321 anncaannnn taanttnccc ccnnna
//
