LOCUS       sequence-237955-       12743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial genome editing plasmid with kanamycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437."
COMMENT     Sequence Label: pCpf1
FEATURES             Location/Qualifiers
     source          1..12743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3923..3957
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(4025..4321)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4171..4193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4185..4202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4186..4202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4212..4231
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4212..4230
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4278..4298)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4278..4296)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4317..4333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4317..4333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4330..4352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4341..4357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4365..4371,4372..4389,4390..4395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4410..4431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(5186..6001)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5186..6001)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5909..5928
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5993..6012
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6859..7253
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7595..7682
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8921..9892
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             10403..11242
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             11229..12080
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     12590..12609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(12713..12718,12719..12736,12737..12742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggaggtcttt atcatgtcaa tttatcaaga atttgttaat aaatatagtt taagtaaaac
       61 tctaagattt gagttaatcc cacagggtaa aacacttgaa aacataaaag caagaggttt
      121 gattttagat gatgagaaaa gagctaaaga ctacaaaaag gctaaacaaa taattgataa
      181 atatcatcag ttttttatag aggagatatt aagttcggtt tgtattagcg aagatttatt
      241 acaaaactat tctgatgttt attttaaact taaaaagagt gatgatgata atctacaaaa
      301 agattttaaa agtgcaaaag atacgataaa gaaacaaata tctgaatata taaaggactc
      361 agagaaattt aagaatttgt ttaatcaaaa ccttatcgat gctaaaaaag ggcaagagtc
      421 agatttaatt ctatggctaa agcaatctaa ggataatggt atagaactat ttaaagccaa
      481 tagtgatatc acagatatag atgaggcgtt agaaataatc aaatctttta aaggttggac
      541 aacttatttt aagggttttc atgaaaatag aaaaaatgtt tatagtagca atgatattcc
      601 tacatctatt atttatagga tagtagatga taatttgcct aaatttctag aaaataaagc
      661 taagtatgag agtttaaaag acaaagctcc agaagctata aactatgaac aaattaaaaa
      721 agatttggca gaagagctaa cctttgatat tgactacaaa acatctgaag ttaatcaaag
      781 agttttttca cttgatgaag tttttgagat agcaaacttt aataattatc taaatcaaag
      841 tggtattact aaatttaata ctattattgg tggtaaattt gtaaatggtg aaaatacaaa
      901 gagaaaaggt ataaatgaat atataaatct atactcacag caaataaatg ataaaacact
      961 caaaaaatat aaaatgagtg ttttatttaa gcaaatttta agtgatacag aatctaaatc
     1021 ttttgtaatt gataagttag aagatgatag tgatgtagtt acaacgatgc aaagttttta
     1081 tgagcaaata gcagctttta aaacagtaga agaaaaatct attaaagaaa cactatcttt
     1141 attatttgat gatttaaaag ctcaaaaact tgatttgagt aaaatttatt ttaaaaatga
     1201 taaatctctt actgatctat cacaacaagt ttttgatgat tatagtgtta ttggtacagc
     1261 ggtactagaa tatataactc aacaaatagc acctaaaaat cttgataacc ctagtaagaa
     1321 agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa tacttatctc tagaaactat
     1381 aaagcttgcc ttagaagaat ttaataagca tagagatata gataaacagt gtaggtttga
     1441 agaaatactt gcaaactttg cggctattcc gatgatattt gatgaaatag ctcaaaacaa
     1501 agacaatttg gcacagatat ctatcaaata tcaaaatcaa ggtaaaaaag acctacttca
     1561 agctagtgcg gaagatgatg ttaaagctat caaggatctt ttagatcaaa ctaataatct
     1621 cttacataaa ctaaaaatat ttcatattag tcagtcagaa gataaggcaa atattttaga
     1681 caaggatgag catttttatc tagtatttga ggagtgctac tttgagctag cgaatatagt
     1741 gcctctttat aacaaaatta gaaactatat aactcaaaag ccatatagtg atgagaaatt
     1801 taagctcaat tttgagaact cgactttggc taatggttgg gataaaaata aagagcctga
     1861 caatacggca attttattta tcaaagatga taaatattat ctgggtgtga tgaataagaa
     1921 aaataacaaa atatttgatg ataaagctat caaagaaaat aaaggcgagg gttataaaaa
     1981 aattgtttat aaacttttac ctggcgcaaa taaaatgtta cctaaggttt tcttttctgc
     2041 taaatctata aaattttata atcctagtga agatatactt agaataagaa atcattccac
     2101 acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa tttgagttta atattgaaga
     2161 ttgccgaaaa tttatagatt tttataaaca gtctataagt aagcatccgg agtggaaaga
     2221 ttttggattt agattttctg atactcaaag atataattct atagatgaat tttatagaga
     2281 agttgaaaat caaggctaca aactaacttt tgaaaatata tcagagagct atattgatag
     2341 cgtagttaat cagggtaaat tgtacctatt ccaaatctat aataaagatt tttcagctta
     2401 tagcaaaggg cgaccaaatc tacatacttt atattggaaa gcgctgtttg atgagagaaa
     2461 tcttcaagat gtggtttata agctaaatgg tgaggcagag cttttttatc gtaaacaatc
     2521 aatacctaaa aaaatcactc acccagctaa agaggcaata gctaataaaa acaaagataa
     2581 tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa gataaacgct ttactgaaga
     2641 taagtttttc tttcactgtc ctattacaat caattttaaa tctagtggag ctaataagtt
     2701 taatgatgaa atcaatttat tgctaaaaga aaaagcaaat gatgttcata tattaagtat
     2761 agatagaggt gaaagacatt tagcttacta tactttggta gatggtaaag gcaatatcat
     2821 caaacaagat actttcaaca tcattggtaa tgatagaatg aaaacaaact accatgataa
     2881 gcttgctgca atagagaaag atagggattc agctaggaaa gactggaaaa agataaataa
     2941 catcaaagag atgaaagagg gctatctatc tcaggtagtt catgaaatag ctaagctagt
     3001 tatagagtat aatgctattg tggtttttga ggatttaaat tttggattta aaagagggcg
     3061 tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa atgctaattg agaaactaaa
     3121 ctatctagtt ttcaaagata atgagtttga taaaactggg ggagtgctta gagcttatca
     3181 gctaacagca ccttttgaga cttttaaaaa gatgggtaaa caaacaggta ttatctacta
     3241 tgtaccagct ggttttactt caaaaatttg tcctgtaact ggttttgtaa atcagttata
     3301 tcctaagtat gaaagtgtca gcaaatctca agagttcttt agtaagtttg acaagatttg
     3361 ttataacctt gataagggct attttgagtt tagttttgat tataaaaact ttggtgacaa
     3421 ggctgccaaa ggcaagtgga ctatagctag ctttgggagt agattgatta actttagaaa
     3481 ttcagataaa aatcataatt gggatactcg agaagtttat ccaactaaag agttggagaa
     3541 attgctaaaa gattattcta tcgaatatgg gcatggcgaa tgtatcaaag cagctatttg
     3601 cggtgagagc gacaaaaagt tttttgctaa gctaactagt gtcctaaata ctatcttaca
     3661 aatgcgtaac tcaaaaacag gtactgagtt agattatcta atttcaccag tagcagatgt
     3721 aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat atgcctcaag atgctgatgc
     3781 caatggtgct tatcatattg ggctaaaagg tctgatgcta ctaggtagga tcaaaaataa
     3841 tcaagagggc aaaaaactca atttggttat caaaaatgaa gagtattttg agttcgtgca
     3901 gaataggaat aactaagaat tcttgacagc tagctcagtc ctaggtataa tgctagcgct
     3961 gatttaggca aaaacgggtc taagaacttt aaataatttc tactgttgta gatgagagca
     4021 ggtgtcagcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     4081 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4141 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4201 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc cccctcgagg
     4261 tcctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     4321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4441 cgctcactgc ccgctttcca cccacctgca aaagtctaag aactttaaat aatttctact
     4501 gttgtagatg ctactattcc tgtgccttca gataattcag tctaagaact ttaaataatt
     4561 tctactgttg tagatgtcta gagccttttg tattagtagc cggtctaaga actttaaata
     4621 atttctactg ttgtagatta gcgatttatg aaggtcattt ttttgtctag ctttaatgcg
     4681 gtagttggta ccgtcgatgg cagaaattcg atatctagat ctcatggatc cgcaatggca
     4741 acaacgttgc gctcgactct agaggatctg taatccgggc agcgcaacgg aacattcatc
     4801 agtgtaaaaa tggaatcaat aaagccctgc gcagcgcgca gggtcagcct gaatacgcgt
     4861 ttaatgacca gcacagtcgt gatggcaagg tcagaatagc gctgaggtct gcctcgtgaa
     4921 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg
     4981 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc
     5041 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
     5101 aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt
     5161 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     5221 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     5281 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     5341 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     5401 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt
     5461 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     5521 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     5581 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     5641 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     5701 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     5761 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     5821 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     5881 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     5941 catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca
     6001 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     6061 ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa
     6121 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt
     6181 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca
     6241 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag
     6301 caacaccttc ttcacgaggc agacctcagc gctattctga ccttgccatc acgactgtgc
     6361 tggtcattaa acgcgtattc aggctgaccc tgcgcgctgc gcagggcttt attgattcca
     6421 tttttacact gatgaatgtt ccgttgcgct gcccggatta cagctgaaag cgaccaggtg
     6481 ctcggcgtgg caagactcgc agcgaacccg tagaaagcca tgctccagcc gcccgcattg
     6541 gagaaattct tcaaattccc gttgcacata gcccggcaat tcctttccct gctctgccat
     6601 aagcgcagcg aatgccgggt aatactcgtc aacgatctga tagagaaggg tttgctcggg
     6661 tcggtggctc tggtaacgac cagtatcccg atcccggctg gccgtcctgg ccgccacatg
     6721 aggcatgttc cgcgtccttg caatactgtg tttacataca gtctatcgct tagcggaaag
     6781 ttcttttacc ctcagccgaa atgcctgccg ttgctagaca ttgccagcca gtgcccgtca
     6841 ctcccgtact aactgtcacg aacccctgca ataactgtca cgcccccctg caataactgt
     6901 cacgaacccc tgcaataact gtcacgcccc caaacctgca aacccagcag gggcgggggc
     6961 tggcggggtg ttggaaaaat ccatccatga ttatctaaga ataatccact aggcgcggtt
     7021 atcagcgccc ttgtggggcg ctgctgccct tgcccaatat gcccggccag aggccggata
     7081 gctggtctat tcgctgcgct aggctacaca ccgccccacc gctgcgcggc agggggaaag
     7141 gcgggcaaag cccgctaaac cccacaccaa accccgcaga aatacgctgg agcgctttta
     7201 gccgctttag cggcctttcc ccctacccga agggtggggg cgcgtgtgca gccccgcagg
     7261 gcctgtctcg gtcgatcatt cagcccggct catccttctg gcgtggcggc agaccgaaca
     7321 aggcgcggtc gtggtcgcgt tcaaggtacg catccattgc cgccatgagc cgatcctccg
     7381 gccactcgct gctgttcacc ttggccaaaa tcatggcccc caccagcacc ttgcgccttg
     7441 tttcgttctt gcgctcttgc tgctgttccc ttgcccgcac ccgctgaatt tcggcattga
     7501 ttcgcgctcg ttgttcttcg agcttggcca gccgatccgc cgccttgttg ctccccttaa
     7561 ccatcttgac accccattgt taatgtgctg tctcgtaggc tatcatggag gcacagcggc
     7621 ggcaatcccg accctacttt gtaggggagg gcgcacttac cggtttctct tcgagaaact
     7681 ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa
     7741 gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa ccggcagcag
     7801 gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag gcaagtatgc
     7861 ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt tcgtcgagcg
     7921 gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa
     7981 ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg cgctggcgtc
     8041 cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg ccatccatgc
     8101 cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca atgacggcat
     8161 cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg agaagggcgg
     8221 ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg
     8281 ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca ttgaccacag
     8341 aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt
     8401 ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga acatcgacac
     8461 cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc atgaacgcaa
     8521 tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc gaaccgctgg
     8581 cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag acccagcagg
     8641 ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg gcggcatggg
     8701 tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg
     8761 cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg ctggcttcca
     8821 tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg acgccactga
     8881 caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg acaggacttt
     8941 gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata tcggcgtcag
     9001 ggacgccacc accggccaga tgatgaaccg ggaatggtca gccgccgaag tgctccagaa
     9061 cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca ggcccgccga
     9121 gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc tggatgacat
     9181 gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga actatcaggc
     9241 atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg cccggacgct
     9301 ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg gccgcttggc
     9361 gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc cgtgggtgct
     9421 gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc agcaggctgg
     9481 ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg ccagcctcga
     9541 actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt accgcagcga
     9601 gatggccggg ctggtcaagc gcttcggtga tgacctcagc aagtgcgact ttatcgccgc
     9661 gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca tggccgaggc
     9721 cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg agcgcaccgt
     9781 cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg cacgggcacc
     9841 ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt agtgcttgcg
     9901 ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt ttatggaata
     9961 cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct attggttgcc
    10021 cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag tcgctgatat
    10081 ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca agcctttcgg
    10141 ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag accggcgacc
    10201 tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg aaggccaaag
    10261 ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg cgcgagtgcc
    10321 tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc taaccaccaa
    10381 ctgcctgcaa aggaggcaat caatggctac ccataagcct atcaatattc tggaggcgtt
    10441 cgcagcagcg ccgccaccgc tggactacgt tttgcccaac atggtggccg gtacggtcgg
    10501 ggcgctggtg tcgcccggtg gtgccggtaa atccatgctg gccctgcaac tggccgcaca
    10561 gattgcaggc gggccggatc tgctggaggt gggcgaactg cccaccggcc cggtgatcta
    10621 cctgcccgcc gaagacccgc ccaccgccat tcatcaccgc ctgcacgccc ttggggcgca
    10681 cctcagcgcc gaggaacggc aagccgtggc tgacggcctg ctgatccagc cgctgatcgg
    10741 cagcctgccc aacatcatgg ccccggagtg gttcgacggc ctcaagcgcg ccgccgaggg
    10801 ccgccgcctg atggtgctgg acacgctgcg ccggttccac atcgaggaag aaaacgccag
    10861 cggccccatg gcccaggtca tcggtcgcat ggaggccatc gccgccgata ccgggtgctc
    10921 tatcgtgttc ctgcaccatg ccagcaaggg cgcggccatg atgggcgcag gcgaccagca
    10981 gcaggccagc cggggcagct cggtactggt cgataacatc cgctggcagt cctacctgtc
    11041 gagcatgacc agcgccgagg ccgaggaatg gggtgtggac gacgaccagc gccggttctt
    11101 cgtccgcttc ggtgtgagca aggccaacta tggcgcaccg ttcgctgatc ggtggttcag
    11161 gcggcatgac ggcggggtgc tcaagcccgc cgtgctggag aggcagcgca agagcaaggg
    11221 ggtgccccgt ggtgaagcct aagaacaagc acagcctcag ccacgtccgg cacgacccgg
    11281 cgcactgtct ggcccccggc ctgttccgtg ccctcaagcg gggcgagcgc aagcgcagca
    11341 agctggacgt gacgtatgac tacggcgacg gcaagcggat cgagttcagc ggcccggagc
    11401 cgctgggcgc tgatgatctg cgcatcctgc aagggctggt ggccatggct gggcctaatg
    11461 gcctagtgct tggcccggaa cccaagaccg aaggcggacg gcagctccgg ctgttcctgg
    11521 aacccaagtg ggaggccgtc accgctgatg ccatggtggt caaaggtagc tatcgggcgc
    11581 tggcaaagga aatcggggca gaggtcgata gtggtggggc gctcaagcac atacaggact
    11641 gcatcgagcg cctttggaag gtatccatca tcgcccagaa tggccgcaag cggcaggggt
    11701 ttcggctgct gtcggagtac gccagcgacg aggcggacgg gcgcctgtac gtggccctga
    11761 accccttgat cgcgcaggcc gtcatgggtg gcggccagca tgtgcgcatc agcatggacg
    11821 aggtgcgggc gctggacagc gaaaccgccc gcctgctgca ccagcggctg tgtggctgga
    11881 tcgaccccgg caaaaccggc aaggcttcca tagatacctt gtgcggctat gtctggccgt
    11941 cagaggccag tggttcgacc atgcgcaagc gccgccagcg ggtgcgcgag gcgttgccgg
    12001 agctggtcgc gctgggctgg acggtaaccg agttcgcggc gggcaagtac gacatcaccc
    12061 ggcccaaggc ggcaggctga ccccccccac tctattgtaa acaagacatt tttatctttt
    12121 atattcaatg gcttattttc ctgctaattg gtaataccat gaaaaatacc atgctcagaa
    12181 aaggcttaac aatattttga aaaattgcct actgagcgct gccgcacagc tccataggcc
    12241 gctttcctgg ctttgcttcc agatgtatgc tcttctgctc ctgcaggcat cgtggtgtca
    12301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    12601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc actttacact
    12721 ttatgcttcc ggctcgtatg tta
//
