LOCUS       sequence-237956-       14822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial genome editing plasmid with kanamycin resistance marker 
            and using sacB as a counter-selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14822)
  AUTHORS   Niu TC, Lin GM, Xie LR, Wang ZQ, Xing WY, Zhang JY, Zhang CC
  TITLE     Expanding the potential of CRISPR-Cpf1 based genome editing 
            technology in the cyanobacterium Anabaena PCC 7120.
  JOURNAL   ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
  PUBMED    30525474
REFERENCE   2  (bases 1 to 14822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437."
COMMENT     Sequence Label: pCpf1b
FEATURES             Location/Qualifiers
     source          1..14822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        3954..3988
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             complement(4056..4352)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
     primer_bind     4202..4224
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4216..4233
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4217..4233
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4243..4262
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4243..4261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4309..4329)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4309..4327)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4348..4364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4348..4364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4361..4383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4372..4388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4396..4402,4403..4420,4421..4426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4441..4462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(5217..6032)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5217..6032)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5940..5959
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6024..6043
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6890..7284
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7626..7713
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8952..9923
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             10434..11273
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             11260..12111
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     promoter        12342..12787
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(12788..12874,12875..14209)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     14700..14719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgtca atttatcaag
       61 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta
      121 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag
      181 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat
      241 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac
      301 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa
      361 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa
      421 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta
      481 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt
      541 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata
      601 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg
      661 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc
      721 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata
      781 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga
      841 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg
      901 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc
      961 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta
     1021 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata
     1081 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag
     1141 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac
     1201 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag
     1261 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag
     1321 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg
     1381 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc
     1441 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc
     1501 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat
     1561 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta
     1621 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta
     1681 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg
     1741 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata
     1801 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg
     1861 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg
     1921 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta
     1981 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa
     2041 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg
     2101 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag
     2161 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac
     2221 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa
     2281 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt
     2341 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat
     2401 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt
     2461 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg
     2521 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta
     2581 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg
     2641 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa
     2701 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag
     2761 aaaaagcaaa tgatgttcat atattaagta tagatagagg tgaaagacat ttagcttact
     2821 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta
     2881 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt
     2941 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat
     3001 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg
     3061 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa
     3121 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg
     3181 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa
     3241 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt
     3301 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc
     3361 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt
     3421 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta
     3481 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc
     3541 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg
     3601 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta
     3661 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt
     3721 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg
     3781 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag
     3841 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta
     3901 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaagaa ttcttgacag
     3961 ctagctcagt cctaggtata atgctagcgc tgatttaggc aaaaacgggt ctaagaactt
     4021 taaataattt ctactgttgt agatgagagc aggtgtcagc cgctacaggg cgcgtcccat
     4081 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     4141 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     4201 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     4261 ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc ttttgttccc tttagtgagg
     4321 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc acccacctgc
     4501 aaaagtctaa gaactttaaa taatttctac tgttgtagat gctactattc ctgtgccttc
     4561 agataattca gtctaagaac tttaaataat ttctactgtt gtagatgtct agagcctttt
     4621 gtattagtag ccggtctaag aactttaaat aatttctact gttgtagatt agcgatttat
     4681 gaaggtcatt tttttgtcta gctttaatgc ggtagttggt accgtcgatg gcagaaattc
     4741 gatatctaga tctcatggat ccgcaatggc aacaacgttg cgctcgactc tagaggatct
     4801 gtaatccggg cagcgcaacg gaacattcat cagtgtaaaa atggaatcaa taaagccctg
     4861 cgcagcgcgc agggtcagcc tgaatacgcg tttaatgacc agcacagtcg tgatggcaag
     4921 gtcagaatag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg
     4981 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta
     5041 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
     5101 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg
     5161 tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag
     5221 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     5281 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     5341 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     5401 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     5461 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     5521 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     5581 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     5641 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     5701 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     5761 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     5821 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     5881 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     5941 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     6001 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     6061 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     6121 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     6181 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
     6241 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat
     6301 gatggggcga ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag
     6361 cgctattctg accttgccat cacgactgtg ctggtcatta aacgcgtatt caggctgacc
     6421 ctgcgcgctg cgcagggctt tattgattcc atttttacac tgatgaatgt tccgttgcgc
     6481 tgcccggatt acagctgaaa gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc
     6541 gtagaaagcc atgctccagc cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat
     6601 agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg taatactcgt
     6661 caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga ccagtatccc
     6721 gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt
     6781 gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga aatgcctgcc
     6841 gttgctagac attgccagcc agtgcccgtc actcccgtac taactgtcac gaacccctgc
     6901 aataactgtc acgcccccct gcaataactg tcacgaaccc ctgcaataac tgtcacgccc
     6961 ccaaacctgc aaacccagca ggggcggggg ctggcggggt gttggaaaaa tccatccatg
     7021 attatctaag aataatccac taggcgcggt tatcagcgcc cttgtggggc gctgctgccc
     7081 ttgcccaata tgcccggcca gaggccggat agctggtcta ttcgctgcgc taggctacac
     7141 accgccccac cgctgcgcgg cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca
     7201 aaccccgcag aaatacgctg gagcgctttt agccgcttta gcggcctttc cccctacccg
     7261 aagggtgggg gcgcgtgtgc agccccgcag ggcctgtctc ggtcgatcat tcagcccggc
     7321 tcatccttct ggcgtggcgg cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac
     7381 gcatccattg ccgccatgag ccgatcctcc ggccactcgc tgctgttcac cttggccaaa
     7441 atcatggccc ccaccagcac cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc
     7501 cttgcccgca cccgctgaat ttcggcattg attcgcgctc gttgttcttc gagcttggcc
     7561 agccgatccg ccgccttgtt gctcccctta accatcttga caccccattg ttaatgtgct
     7621 gtctcgtagg ctatcatgga ggcacagcgg cggcaatccc gaccctactt tgtaggggag
     7681 ggcgcactta ccggtttctc ttcgagaaac tggcctaacg gccacccttc gggcggtgcg
     7741 ctctccgagg gccattgcat ggagccgaaa agcaaaagca acagcgaggc agcatggcga
     7801 tttatcacct tacggcgaaa accggcagca ggtcgggcgg ccaatcggcc agggccaagg
     7861 ccgactacat ccagcgcgaa ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg
     7921 aatccgggca catgccggag ttcgtcgagc ggcccgccga ctactgggat gctgccgacc
     7981 tgtatgaacg cgccaatggg cggctgttca aggaggtcga atttgccctg ccggtcgagc
     8041 tgaccctcga ccagcagaag gcgctggcgt ccgagttcgc ccagcacctg accggtgccg
     8101 agcgcctgcc gtatacgctg gccatccatg ccggtggcgg cgagaacccg cactgccacc
     8161 tgatgatctc cgagcggatc aatgacggca tcgagcggcc cgccgctcag tggttcaagc
     8221 ggtacaacgg caagaccccg gagaagggcg gggcacagaa gaccgaagcg ctcaagccca
     8281 aggcatggct tgagcagacc cgcgaggcat gggccgacca tgccaaccgg gcattagagc
     8341 gggctggcca cgacgcccgc attgaccaca gaacacttga ggcgcagggc atcgagcgcc
     8401 tgcccggtgt tcacctgggg ccgaacgtgg tggagatgga aggccggggc atccgcaccg
     8461 accgggcaga cgtggccctg aacatcgaca ccgccaacgc ccagatcatc gacttacagg
     8521 aataccggga ggcaatagac catgaacgca atcgacagag tgaagaaatc cagaggcatc
     8581 aacgagttag cggagcagat cgaaccgctg gcccagagca tggcgacact ggccgacgaa
     8641 gcccggcagg tcatgagcca gacccagcag gccagcgagg cgcaggcggc ggagtggctg
     8701 aaagcccagc gccagacagg ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta
     8761 gccgccgagg tgagcagcgc cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg
     8821 aagctatggc taaccgtgat gctggcttcc atgatgccta cggtggtgct gctgatcgca
     8881 tcgttgctct tgctcgacct gacgccactg acaaccgagg acggctcgat ctggctgcgc
     8941 ttggtggccc gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca
     9001 tgggctgtga gcgcttcgat atcggcgtca gggacgccac caccggccag atgatgaacc
     9061 gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc
     9121 agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg
     9181 acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg
     9241 tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg
     9301 gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca
     9361 gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca
     9421 ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca
     9481 ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc
     9541 aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc
     9601 ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtg
     9661 atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg
     9721 ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg
     9781 gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc
     9841 agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg
     9901 gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt
     9961 actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc
    10021 tgatcaaaag tgacaagggc tattggttgc ccggtggctt tggttatacg tcaaacaagg
    10081 ccgaggctgg ccgcttttca gtcgctgata tggccagcct taaccttgac ggctgcacct
    10141 tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa gtttctcggt gactgatatg
    10201 aaagaccaaa aggacaagca gaccggcgac ctgctggcca gccctgacgc tgtacgccaa
    10261 gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc
    10321 gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg
    10381 ggtagtgacc ccgccagcgc ctaaccacca actgcctgca aaggaggcaa tcaatggcta
    10441 cccataagcc tatcaatatt ctggaggcgt tcgcagcagc gccgccaccg ctggactacg
    10501 ttttgcccaa catggtggcc ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta
    10561 aatccatgct ggccctgcaa ctggccgcac agattgcagg cgggccggat ctgctggagg
    10621 tgggcgaact gcccaccggc ccggtgatct acctgcccgc cgaagacccg cccaccgcca
    10681 ttcatcaccg cctgcacgcc cttggggcgc acctcagcgc cgaggaacgg caagccgtgg
    10741 ctgacggcct gctgatccag ccgctgatcg gcagcctgcc caacatcatg gccccggagt
    10801 ggttcgacgg cctcaagcgc gccgccgagg gccgccgcct gatggtgctg gacacgctgc
    10861 gccggttcca catcgaggaa gaaaacgcca gcggccccat ggcccaggtc atcggtcgca
    10921 tggaggccat cgccgccgat accgggtgct ctatcgtgtt cctgcaccat gccagcaagg
    10981 gcgcggccat gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg
    11041 tcgataacat ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat
    11101 ggggtgtgga cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact
    11161 atggcgcacc gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg
    11221 ccgtgctgga gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag
    11281 cacagcctca gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt
    11341 gccctcaagc ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac
    11401 ggcaagcgga tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg
    11461 caagggctgg tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc
    11521 gaaggcggac ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgat
    11581 gccatggtgg tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat
    11641 agtggtgggg cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc
    11701 atcgcccaga atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac
    11761 gaggcggacg ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt
    11821 ggcggccagc atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc
    11881 cgcctgctgc accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc
    11941 atagatacct tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag
    12001 cgccgccagc gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc
    12061 gagttcgcgg cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca
    12121 ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt
    12181 ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc
    12241 tactgagcgc tgccgcacag ctccataggc cgctttcctg gctttgcttc cagatgtatg
    12301 ctcttctgct ctagctagag gatcgatcct ttttaaccca tcacatatac ctgccgttca
    12361 ctattattta gtgaaatgag atattatgat attttctgaa ttgtgattaa aaaggcaact
    12421 ttatgcccat gcaacagaaa ctataaaaaa tacagagaat gaaaagaaac agatagattt
    12481 tttagttctt taggcccgta gtctgcaaat ccttttatga ttttctatca aacaaaagag
    12541 gaaaatagac cagttgcaat ccaaacgaga gtctaataga atgaggtcga aaagtaaatc
    12601 gcgcgggttt gttactgata aagcaggcaa gacctaaaat gtgtaaaggg caaagtgtat
    12661 actttggcgt caccccttac atattttagg tcttttttta ttgtgcgtaa ctaacttgcc
    12721 atcttcaaac aggagggctg gaagaagcag accgctaaca cagtacataa aaaaggagac
    12781 atgaacgatg aacatcaaaa agtttgcaaa acaagcaaca gtattaacct ttactaccgc
    12841 actgctggca ggaggcgcaa ctcaagcgtt tgcgaaagaa acgaaccaaa agccatataa
    12901 ggaaacatac ggcatttccc atattacacg ccatgatatg ctgcaaatcc ctgaacagca
    12961 aaaaaatgaa aaatatcaag ttcctgaatt cgattcgtcc acaattaaaa atatctcttc
    13021 tgcaaaaggc ctggacgttt gggacagctg gccattacaa aacgctgacg gcactgtcgc
    13081 aaactatcac ggctaccaca tcgtctttgc attagccgga gatcctaaaa atgcggatga
    13141 cacatcgatt tacatgttct atcaaaaagt cggcgaaact tctattgaca gctggaaaaa
    13201 cgctggccgc gtctttaaag acagcgacaa attcgatgca aatgattcta tcctaaaaga
    13261 ccaaacacaa gaatggtcag gttcagccac atttacatct gacggaaaaa tccgtttatt
    13321 ctacactgat ttctccggta aacattacgg caaacaaaca ctgacaactg cacaagttaa
    13381 cgtatcagca tcagacagct ctttgaacat caacggtgta gaggattata aatcaatctt
    13441 tgacggtgac ggaaaaacgt atcaaaatgt acagcagttc atcgatgaag gcaactacag
    13501 ctcaggcgac aaccatacgc tgagagatcc tcactacgta gaagataaag gccacaaata
    13561 cttagtattt gaagcaaaca ctggaactga agatggctac caaggcgaag aatctttatt
    13621 taacaaagca tactatggca aaagcacatc attcttccgt caagaaagtc aaaaacttct
    13681 gcaaagcgat aaaaaacgca cggctgagtt agcaaacggc gctctcggta tgattgagct
    13741 aaacgatgat tacacactga aaaaagtgat gaaaccgctg attgcatcta acacagtaac
    13801 agatgaaatt gaacgcgcga acgtctttaa aatgaacggc aaatggtacc tgttcactga
    13861 ctcccgcgga tcaaaaatga cgattgacgg cattacgtct aacgatattt acatgcttgg
    13921 ttatgtttct aattctttaa ctggcccata caagccgctg aacaaaactg gccttgtgtt
    13981 aaaaatggat cttgatccta acgatgtaac ctttacttac tcacacttcg ctgtacctca
    14041 agcgaaagga aacaatgtcg tgattacaag ctatatgaca aacagaggat tctacgcaga
    14101 caaacaatca acgtttgcgc caagcttcct gctgaacatc aaaggcaaga aaacatctgt
    14161 tgtcaaagac agcatccttg aacaaggaca attaacagtt aacaaataaa aacgcaaaag
    14221 aaaatgccga tatcctattg gcattttctt ttatttctta tcaacataaa ggtgaatccc
    14281 atatgaacta tataaaagca ggcaaatggc taaccgtatt cctaaccttt tggtaatgac
    14341 tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagcat
    14401 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    14461 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    14521 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    14581 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    14641 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
    14701 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    14761 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    14821 ac
//
