LOCUS       sequence-237985-        9764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible GFP (N-terminal tag) SCAF8 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pFRT/TO/GFP-SCAF8 (CRISPR_resistant)
FEATURES             Location/Qualifiers
     source          1..9764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(756..758,759..761,762..1472)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(756..758,759..761,762..1472)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(801..822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1062..1081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1409..1430
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1479..1503
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5326..5350)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5403..5444
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5482..5499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5488..5712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5845..5864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5996..6043
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6051..7073
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6051..7073
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7203..7324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7240..7259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7294..7313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7373..7389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7373..7389)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7386..7408)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7397..7413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7421..7427,7428..7445,7446..7451))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7466..7487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7604..7621)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7775..8363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7855..7874)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8534..9325,9326..9394))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9157..9176
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9395..9499)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9574..9593)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccatggt gagcaagggc gaggagctgt
      781 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      841 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      901 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      961 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1021 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1081 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1141 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1201 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1261 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1321 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1381 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1441 ggatcactct cggcatggac gagctgtaca agaagcttac aagtttgtac aaaaaagcag
     1501 gcttcaccat ggaggccgtg aagaccttca atagcgagtt gtattccctg aatgactata
     1561 aaccacccat ttcgaaagcg aaaatgaccc aaattactaa ggcagccatc aaagctatta
     1621 agttctataa acatgtggta cagagtgttg agaagtttat tcagaaatgt aaaccagaat
     1681 acaaagtacc tggactttat gttattgact ccattgtgcg acaatcccga catcagtttg
     1741 gtcaagaaaa ggatgtgttt gcacccagat ttagtaataa catcattagc actttccaga
     1801 atttatatcg ttgccctggg gatgacaaga gtaaaatagt gagagtacta aacttatggc
     1861 agaagaataa tgtatttaag agtgagatta ttcagcccct tttggatatg gcagccggga
     1921 ttccgcctcc agttgtcaca cctgttttgg ccagcactac cactgctatg agcaatactc
     1981 caggaactcc agtcacgccg gtcacgcctg ccaatgtggt tcagggattg ccagacccat
     2041 gggtatctca gataacaaat acagatacac ttgcggctgt agctcagatc ttgcaaagtc
     2101 ctcaaggcca gcagcttcaa caattaatac aaaccttaca gatacaacaa cagaagcccc
     2161 agccttccat tctgcaggcc ctagatgctg gtcttgttgt tcagttgcaa gctcttacgg
     2221 cacaacttac agctgcagct gcagctgcca acactcttac tcccttagaa cagggagtct
     2281 cctttaacaa gaagttgatg gataggtttg attttgggga agactctgag catagtgaag
     2341 aacccaaaaa ggaaattcca gcttcacaac tttctcacgt ttcagaatct gtgaacaatt
     2401 ccatttttca tcagatagca gaacaactac aacagcaaaa cctagaacat ctcagacagc
     2461 agctcttgga gcagcaacag cctcaaaagg ccactcctca ggatagtcag gaaggaacct
     2521 ttgggtcaga gcattcagcg tcaccatcac aagggagtag tcagcagcat tttcttgaac
     2581 ctgaagtcaa tttggatgat tccatagata ttcagcaaca ggatatggat atagatgaag
     2641 ggcaagatgg agtggaagag gaggtctttg aacaagaagc taagaaagtg gcggttcgct
     2701 caagatcaag aacacattca cgatctcgtt caagatcacc aagaaaacga aggtctaggt
     2761 cacggtctgg ctctagaaag cgtaaacaca gaaagcgatc acgctcccgc tcaagagaaa
     2821 gaaagaggaa atcatcacgg tcgtattcaa gtgaaaggag agccagagaa agggagaaag
     2881 aacgacagaa aaagggatta cctccaatta gatctaaaac actaagtgta tgtagtacta
     2941 ctctctgggt tgggcaagtg gacaagaagg caacacagca agacttaacc aacctgtttg
     3001 aagagtttgg acagattgaa tccattaata tgattcctcc gcgaggctgt gcttatgtct
     3061 gcatggttca tcgacaagat gcatttcgag ctcttcagaa actcagttct ggatcatata
     3121 aaattgggtc caaggtcatt aagatcgctt gggctttaaa caaaggtgta aaaacagaat
     3181 acaaacaatt ctgggatgtg gatcttggag ttacatatat accatgggaa aaagttaaag
     3241 tggatgactt ggaaggtttt gcagaaggag gcatgattga tcaggagact gtaaatactg
     3301 agtgggaaac tgtgaaaagc tcagaacctg ttaaagagac ggtccagaca actcagagcc
     3361 caactccagt tgaaaaggag acagtggtca caacccaggc agaggttttc cctcctcctg
     3421 ttgctatgtt gcagattcca gtggcgccag ccgtgcctac agttagttta gtcccaccag
     3481 catttcctgt gtcgatgccg gttcctcctc ctggattcag tccaatccct ccacctcctt
     3541 ttttaagagc aagttttaac ccttcacaac caccacctgg tttcatgccg cctccagttc
     3601 ccccacctgt tgtgccaccc cctacgattc caccagtagt accaacatct ttagtgcagc
     3661 cgtcattatc catgacaccg gaaactgtga aagatgttgg atttggtagc cttgttatac
     3721 caggcggttc tgttgccagc aatcttgcta cttccgctct gccagctgga aatgttttta
     3781 atgctccaac taaacaggca gagcctgaag aaaaagtacc tcatcttata gaccaccaga
     3841 tttcttctgg tgaaaacacc agatcagtga ttccaaatga tatttcaagt aatgctgcaa
     3901 ttttaggagg acagccgcca aatgtgacaa gcaattctgg aattctggga gtccaaagac
     3961 caaatgtatc aagtaattct gaaattcttg gggtccggcc atctaatgtt tccagtagtt
     4021 ctgggattat tgcagcccaa ccaccaaata ttctaaataa ctctggaata ttgggaatac
     4081 agccacccag tgtgtcaaat agttctggac ttttgggagt gctaccccca aatataccta
     4141 acaattctgg acttgtagga gtacagccac caaatgttcc aaatactcct ggacttctgg
     4201 gaacacagcc accagctgga cctcaaaact tacccccttt aagtatccct aatcaaagga
     4261 tgcccacaat gccaatgtta gacattcgtc cgggactaat accacaggca cctgggccaa
     4321 gattcccttt aatacagcct ggaattccac cccaacgggg aatcccaccc ccatcggtac
     4381 ttgattcagc tcttcatcca ccaccccgtg gaccttttcc tccaggagat atttttagtc
     4441 aaccagaaag acctttttta gctcctggaa gacaaagcgt agacaatgtt actaacccag
     4501 aaaaaaggat accacttggg aatgataaca ttcaacagga aggagataga gattatcggt
     4561 ttcctcctat agaaaccagg gaaagcatta gtagacctcc ccctgtggat gttagagatg
     4621 tggttgggcg gcctatagat ccaagagaag gtcctggacg gcctccacta gatggtaggg
     4681 atcattttgg aagacctcct gtagatataa gagagaatct tgtgaggcca ggtatagatc
     4741 atcttggtcg aagagaccac tttggcttta atccagagaa gccctggggg catagaggag
     4801 attttgatga gagagagcat cgggttctac cggtctatgg tggtccaaaa ggcttacatg
     4861 aagaaagagg tagatttcgg tctggaaact atcgatttga tcctagaagt ggtccttgga
     4921 accgaggatt tggacaagaa gttcacagag attttgatga ccgcagaaga ccctgggaga
     4981 ggcaaaggga tagggatgac agagattttg atttctgcag agaaatgaat ggaaatcgtc
     5041 ttggacgaga cagaattcaa aacacttggg ttccccctcc tcatgctcgg gtttttgatt
     5101 attttgaagg ggccacttct caacgaaaag gtgataatgt gcctcaggtt aatggtgaaa
     5161 atacagagag acatgctcag ccaccaccta taccagtaca gaatgatcct gaactttatg
     5221 aaaaactgac atcttcaaat gaaataaaca aggagaagag tgacacagtt gctgatatag
     5281 aaagtgaacc agtggtagaa agcacagaaa ctgaggggac ataaaaccca gctttcttgt
     5341 acaaagtggt tgatatccag cacagtggcg gccgctcaag tctagagggc ccgcggttcg
     5401 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgctcgag tctagagggc
     5461 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5521 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5581 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5641 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5701 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt
     6001 tcctattccg aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg
     6061 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     6121 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     6181 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     6241 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     6301 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     6361 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     6421 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     6481 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     6541 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     6601 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     6661 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     6721 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     6781 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     6841 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     6901 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     6961 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     7021 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac
     7081 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7141 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7201 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7321 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7381 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7441 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7501 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7561 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8821 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9121 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     9541 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     9601 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     9661 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     9721 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
