LOCUS       sequence-237998-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, 
            Stewart A, Svejstrup JQ
  TITLE     SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
  JOURNAL   Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038.
  PUBMED    31104839
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            May 10. pii: S0092-8674(19)30457-X. doi: 
            10.1016/j.cell.2019.04.038."
COMMENT     Sequence Label: pDONR223 SCAF8_isoD
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3901..4000)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4017..4036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4018..4036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4041..4057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4041..4057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4488..5279
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5372..5960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5861..5880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6114..6131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6290..6317)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6409..6495)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6544..6566
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6558..6575
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6559..6575
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    join(6591..6612,1..78)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
       61 tgtacaaaaa agcaggcttc accatggagg ccgtgaagac cttcaatagc gagttgtatt
      121 ccctgaatga ctataaacca cccatttcga aagcgaaaat gacccaaatt actaaggcag
      181 ccatcaaagc tattaagttc tataaacatg tggtacagag tgttgagaag tttattcaga
      241 aatgtaaacc agaatacaaa gtacctggac tttatgttat tgactccatt gtgcgacaat
      301 cccgacatca gtttggtcaa gaaaaggatg tgtttgcacc cagatttagt aataacatca
      361 ttagcacttt ccagaattta tatcgttgcc ctggggatga caagagtaaa atagtgagag
      421 tactaaactt atggcagaag aataatgtat ttaagagtga gattattcag ccccttttgg
      481 atatggcagc cgggattccg cctccagttg tcacacctgt tttggccagc actaccactg
      541 ctatgagcaa tactccagga actcctgtga cacctgttac tccggccaat gtggtccaag
      601 gcttacctga tccgtgggta tctcagataa caaatacaga tacacttgcg gctgtagctc
      661 agatcttgca aagtcctcaa ggccagcagc ttcaacaatt aatacaaacc ttacagatac
      721 aacaacagaa gccccagcct tccattctgc aggccctaga tgctggtctt gttgttcagt
      781 tgcaagctct tacggcacaa cttacagctg cagctgcagc tgccaacact cttactccct
      841 tagaacaggg agtctccttt aacaagaagt tgatggatag gtttgatttt ggggaagact
      901 ctgagcatag tgaagaaccc aaaaaggaaa ttccagcttc acaactttct cacgtttcag
      961 aatctgtgaa caattccatt tttcatcaga tagcagaaca actacaacag caaaacctag
     1021 aacatctcag acagcagctc ttggagcagc aacagcctca aaaggccact cctcaggata
     1081 gtcaggaagg aacctttggg tcagagcatt cagcgtcacc atcacaaggg agtagtcagc
     1141 agcattttct tgaacctgaa gtcaatttgg atgattccat agatattcag caacaggata
     1201 tggatataga tgaagggcaa gatggagtgg aagaggaggt ctttgaacaa gaagctaaga
     1261 aagtggcggt tcgctcaaga tcaagaacac attcacgatc tcgttcaaga tcaccaagaa
     1321 aacgaaggtc taggtcacgg tctggctcta gaaagcgtaa acacagaaag cgatcacgct
     1381 cccgctcaag agaaagaaag aggaaatcat cacggtcgta ttcaagtgaa aggagagcca
     1441 gagaaaggga gaaagaacga cagaaaaagg gattacctcc aattagatct aaaacactaa
     1501 gtgtatgtag tactactctc tgggttgggc aagtggacaa gaaggcaaca cagcaagact
     1561 taaccaacct gtttgaagag tttggacaga ttgaatccat taatatgatt cctccccggg
     1621 gctgtgctta tgtctgcatg gttcatcgac aagatgcatt tcgagctctt cagaaactca
     1681 gttctggatc atataaaatt gggtccaagg tcattaagat cgcttgggct ttaaacaaag
     1741 gtgtaaaaac agaatacaaa caattctggg atgtggatct tggagttaca tatataccat
     1801 gggaaaaagt taaagtggat gacttggaag gttttgcaga aggaggcatg attgatcagg
     1861 agactgtaaa tactgagtgg gaaactgtga aaagctcaga acctgttaaa gagacggtcc
     1921 agacaactca gagcccaact ccagttgaaa aggagacagt ggtcacaacc caggcagagg
     1981 ttttccctcc tcctgttgct atgttgcaga ttccagtggc gccagccgtg cctacagtta
     2041 gtttagtccc accagcattt cctgtgtcga tgccggttcc tcctcctgga ttcagtccaa
     2101 tccctccacc tcctttttta agagcaagtt ttaacccttc acaaccacca cctggtttca
     2161 tgccgcctcc agttccccca cctgttgtgc caccccctac gattccacca gtagtaccaa
     2221 catctttagt gcagccgtca ttatccatga caccggaaac tgtgaaagat gttggatttg
     2281 gtagccttgt tataccaggc ggttctgttg ccagcaatct tgctacttcc gctctgccag
     2341 ctggaaatgt ttttaatgct ccaactaaac aggcagagcc tgaagaaaaa gtacctcatc
     2401 ttatagacca ccagatttct tctggtgaaa acaccagatc agtgattcca aatgatattt
     2461 caagtaatgc tgcaatttta ggaggacagc cgccaaatgt gacaagcaat tctggaattc
     2521 tgggagtcca aagaccaaat gtatcaagta attctgaaat tcttggggtc cggccatcta
     2581 atgtttccag tagttctggg attattgcag cccaaccacc aaatattcta aataactctg
     2641 gaatattggg aatacagcca cccagtgtgt caaatagttc tggacttttg ggagtgctac
     2701 ccccaaatat acctaacaat tctggacttg taggagtaca gccaccaaat gttccaaata
     2761 ctcctggact tctgggaaca cagccaccag ctggacctca aaacttaccc cctttaagta
     2821 tccctaatca aaggatgccc acaatgccaa tgttagacat tcgtccggga ctaataccac
     2881 aggcacctgg gccaagattc cctttaatac agcctggaat tccaccccaa cggggaatcc
     2941 cacccccatc ggtacttgat tcagctcttc atccaccacc ccgtggacct tttcctccag
     3001 gagatatttt tagtcaacca gaaagacctt ttttagctcc tggaagacaa agcgtagaca
     3061 atgttactaa cccagaaaaa aggataccac ttgggaatga taacattcaa caggaaggag
     3121 atagagatta tcggtttcct cctatagaaa ccagggaaag cattagtaga cctccccctg
     3181 tggatgttag agatgtggtt gggcggccta tagatccaag agaaggtcct ggacggcctc
     3241 cactagatgg tagggatcat tttggaagac ctcctgtaga tataagagag aatcttgtga
     3301 ggccaggtat agatcatctt ggtcgaagag accactttgg ctttaatcca gagaagccct
     3361 gggggcatag aggagatttt gatgagagag agcatcgggt tctaccggtc tatggtggtc
     3421 caaaaggctt acatgaagaa agaggtagat ttcggtctgg aaactatcga tttgatccta
     3481 gaagtggtcc ttggaaccga ggatttggac aagaagttca cagagatttt gatgaccgca
     3541 gaagaccctg ggagaggcaa agggataggg atgacagaga ttttgatttc tgcagagaaa
     3601 tgaatggaaa tcgtcttgga cgagacagaa ttcaaaacac ttgggttccc cctcctcatg
     3661 ctcgggtttt tgattatttt gaaggggcca cttctcaacg aaaaggtgat aatgtgcctc
     3721 aggttaatgg tgaaaataca gagagacatg ctcagccacc acctatacca gtacagaatg
     3781 atcctgaact ttatgaaaaa ctgacatctt caaatgaaat aaacaaggag aagagtgaca
     3841 cagttgctga tatagaaagt gaaccagtgg tagaaagcac agaaactgag gggacataaa
     3901 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     3961 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     4021 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     4081 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     4141 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     4201 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     4261 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     4321 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     4381 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     4441 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     4501 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     4561 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     4621 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     4681 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     4741 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     4801 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     4861 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     4921 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     4981 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     5041 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     5101 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     5161 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     5221 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     5281 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     5341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6001 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6061 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6121 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6181 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6241 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     6301 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     6361 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     6421 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     6481 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     6541 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
     6601 ttttattttg ac
//
