LOCUS       sequence-238042-       14441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged hRPGRIP1L in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Takemaru et al.
  TITLE     Takemaru lab unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EF1alpha-IRES-EGFP-Flag-hRPGRIP1L
FEATURES             Location/Qualifiers
     source          1..14441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..50
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4036..4610,4611..4613,4614..4622)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4203..4220)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4430..4449
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4623..4625,4626..4628,4629..5342)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4623..4625,4626..4628,4629..5339)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4668..4689)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4929..4948)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5276..5297
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5352..5940
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5405..5425)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5823..5834)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6464..6644
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6670..6687)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6676..6900
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6946..7374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7033..7052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7243..7264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7383..7403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7388..7717
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7568..7703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7630..7649
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7765..7812
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7831..8205
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8335..8456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8372..8391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8426..8445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8505..8521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8505..8521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8518..8540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8529..8545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8553..8559,8560..8577,8578..8583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8598..8619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8736..8753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8907..9495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8987..9006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9666..10457,10458..10526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10289..10308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10527..10631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10706..10725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10897..11276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11278..11476
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11431..11451
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11494..11674
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11721..11846
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12339..12572
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12757..12801
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13099..13216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        13263..14441
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          13494..14432
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     14389..14409
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattcggc cattacggcc accatggatt acaaggatga cgacgataag atctcgaatt
       61 cgtctggtcc aactgatgag actgcaggag acttgcctgt gaaagataca ggtctaaacc
      121 tctttggaat gggagggtta caagaaactt caacaacacg gacaatgaag tctcgccagg
      181 cagtgtcacg tgtcagtcgt gaggaactgg aagacagatt tttgcgtttg catgatgaga
      241 acattttact taaacagcat gcccgcaagc aggaggataa aattaaaaga atggccacca
      301 agttaatacg gctagttaat gacaagaaaa gatatgagcg ggttggtggc ggccccaagc
      361 ggctgggacg agatgtggaa atggaagaaa tgattgagca gctgcaagag aaagttcatg
      421 agcttgaaaa acaaaatgaa accctcaaaa acagactgat ttcagccaaa cagcaacttc
      481 aaacccaggg ttacaggcaa actccataca ataatgtaca atctcgtatt aacactgggc
      541 gtagaaaagc aaatgaaaat gctggtttac aggaatgccc caggaaaggt ataaaattcc
      601 aagatgcaga tgtagcagaa actccacatc ccatgtttac aaaatatggc aacagtttac
      661 ttgaagaagc cagaggagaa ataagaaatt tagaaaacgt tattcagtca caaagaggcc
      721 agatagagga gttagagcac ttggctgaga tcctgaaaac tcagttgagg agaaaagaaa
      781 atgaaattga gttatctctt cttcagcttc gagaacagca agctacagat caaaggtcaa
      841 atattcggga caatgtagaa atgattaagc ttcataaaca gctagtagag aaaagcaatg
      901 ctctttcagc aatggaagga aaatttattc agcttcaaga gaagcaaaga actctcagaa
      961 tcagccacga tgctttgatg gcaaatgggg atgaattaaa catgcaactt aaagagcagc
     1021 gtttaaaatg ctgcagtctt gagaaacaat tacattctat gaagttttct gaaagaagaa
     1081 tagaagagct gcaggataga attaatgatt tagaaaagga acgggaactt ttaaaggaaa
     1141 actatgataa actttatgac agtgccttca gtgctgccca tgaagagcaa tggaagttaa
     1201 aggagcaaca gctgaaagtg cagattgctc agctcgagac tgccttgaag tctgacttaa
     1261 cagacaaaac tgaaatcctt gacagattaa aaactgaaag agatcaaaat gaaaaactcg
     1321 ttcaagagaa tagagaacta cagttacagt atctggaaca aaagcaacaa cttgatgaac
     1381 ttaaaaaacg cataaaattg tacaaccagg agaatgatat taatgctgat gaattgagtg
     1441 aagctctcct acttataaag gctcaaaaag aacaaaaaaa tggagacctt tcctttttag
     1501 tgaaagtaga tagtgaaatt aataaagatc tagaacgctc tatgagagag ctgcaagcaa
     1561 ctcatgcaga aacggtgcaa gagctggaaa agacaagaaa catgctaatt atgcaacaca
     1621 aaattaataa agattatcag atggaggttg aggcagtgac ccgtaagatg gaaaatttgc
     1681 agcaagatta tgaactcaaa gtggaacagt atgttcatct tcttgatatc agggctgcac
     1741 gtatccataa actagaagcc caattaaagg atattgccta tgacaccaag cagtacaaat
     1801 ttaaaccaga aatcatgcca gatgactctg ttgatgaatt tggtgaaacc atccacttag
     1861 aacgaggcga aaatctattt gaaatccata tcaacaaagt aaccttttct tctgaagttt
     1921 tacaggcatc tggagataaa gagcctgtca ctttctgtac ctatgctttc tatgattttg
     1981 aactacagac aactcccgta gtgcgaggcc ttcatcccga atataacttc acttctcaat
     2041 atcttgttca tgttaatgac ttatttttgc aatatattca gaagaatact atcacccttg
     2101 aggtccacca ggcttatagc acagaatatg aaacaattgc agcatgtcaa ttaaaatttc
     2161 acgaaattct tgaaaaaagc ggccgaatat tttgtacagc aagtttgatt ggaacaaaag
     2221 gagacatccc aaattttggc acagtggaat actggttccg attaagagtt cccatggatc
     2281 aagcaattcg actttatcga gaaagggcaa aggctttggg gtatataaca tcaaatttta
     2341 aggggccaga gcatatgcag tcgttaagtc agcaagcacc caaaactgct caactcagtt
     2401 ctacagattc cacagatggc aacttaaatg aacttcacat tacaataaga tgttgcaacc
     2461 acctgcagtc ccgagcaagc cacctgcagc cacacccata tgttgtgtac aagttttttg
     2521 attttgcaga ccatgataca gctatcattc ccagtagcaa tgatccacag tttgatgatc
     2581 atatgtattt cccagtgcca atgaatatgg acttggatcg ataccttaag tcagagtctc
     2641 tgagttttta tgtttttgat gatagtgata cccaggagaa tatttacata ggaaaagtca
     2701 atgtgcctct gatttcgttg gcacatgaca ggtgtatctc aggaatattt gagttaacag
     2761 accatcaaaa gcatcctgct ggcaccatcc atgttatatt gaaatggaaa tttgcttacc
     2821 ttccaccaag tggatcaata acaactgaag acttaggaaa tttcattcgc agcgaagagc
     2881 cagaagttgt tcaaagactt cctccagcat cctctgttag cacactagtt ttagcaccaa
     2941 gacctaaacc aagacaacgt ttaacacctg tagataagaa ggtatctttc gtggatatca
     3001 tgccacatca gagtgatgag acttctcctc ctcttgaaga taggaaggaa atttcaccag
     3061 aggtagagca tataccagaa atagaaatta atatgctgac tgttccacat gttcccaagg
     3121 tttcacaaga aggcagtgta gatgaggtaa aagagaatac tgagaaaatg cagcaaggaa
     3181 aagatgatgt gtctttacta tctgaaggtc agcttgcaga acaaagcttg gcatcttctg
     3241 aagatgaaac agaaataaca gaggacttgg aaccagaagt tgaagaggac atgtcagctt
     3301 ctgacagtga tgactgtatt attccaggtc ctatctccaa gaatatcaaa cagagtctcg
     3361 cattgtctcc cgggctgggg tgcagtagcg cgatctcagc tcactgcaac ttccgcctcc
     3421 cgggttccag cgattttcct gcctcagcct cccaagtaga tgggattaca ggtgcctgcc
     3481 accacagcca gccatcagaa aaaattcgga ttgagatcat agctctaagc cttaatgatt
     3541 ctcaagtaac catggatgac actatccaac ggctgtttgt tgagtgtcga ttctacagtc
     3601 ttcctgctga agagacaccc gtgtcacttc caaaacccaa gagtgggcag tgggtctact
     3661 ataactatag caatgtgatc tacgtggata aagaaaacaa caaagcaaag agagacatct
     3721 taaaagctat actacaaaaa caagagatgc ctaatagaag ccttcgcttc accgtggtca
     3781 gtgaccctcc agaggacgag caggacctgg agtgtgagga cattggcgtg gctcacgtcg
     3841 accttgccga catgtttcag gaagggaggg acctcattga gcaaaatatc gatgtttttg
     3901 atgcacgagc agatggtgaa ggtattggca agctcagggt aacagtcgaa gctctccatg
     3961 ccctccagtc tgtctacaag caatacagag atgacttgga ggcttgaggc cgcctcggcc
     4021 atcgataagg atccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     4081 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4141 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4201 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4261 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     4321 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     4381 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     4441 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     4501 tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     4561 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     4621 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4681 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4741 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4801 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4861 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4921 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4981 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5041 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5101 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5161 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5221 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5281 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5341 aaagcggccg caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5401 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5461 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5521 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5581 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5641 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5701 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5761 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5821 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5881 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5941 ctgcctgcag gtttgtcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6001 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6061 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6121 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6181 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6241 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6301 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6361 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     6421 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     6661 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6721 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6781 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6841 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6901 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6961 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7021 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7081 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7141 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7201 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7261 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7321 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7381 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7441 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7501 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7561 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7621 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7681 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7741 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7801 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7861 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7921 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7981 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8041 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8101 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8161 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8221 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8281 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8341 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8401 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8461 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8521 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8581 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8641 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8701 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8761 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9661 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9721 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9781 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9841 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9901 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9961 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10021 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10081 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10141 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10201 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10261 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10321 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10381 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10441 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10501 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10561 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10621 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    10681 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10741 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    10801 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    10861 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    10921 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    10981 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    11041 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    11101 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
    11161 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
    11221 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
    11281 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
    11341 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
    11401 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
    11461 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
    11521 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11581 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11641 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    11701 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    11761 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    11821 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    11881 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    11941 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    12001 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    12061 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    12121 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    12181 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    12241 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12301 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12361 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12421 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12481 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    12541 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    12601 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    12661 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    12721 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    12781 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    12841 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    12901 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    12961 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13021 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
    13081 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    13141 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    13201 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
    13261 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgt
    13321 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
    13381 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
    13441 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
    13501 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
    13561 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
    13621 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
    13681 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
    13741 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
    13801 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
    13861 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
    13921 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
    13981 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
    14041 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
    14101 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
    14161 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
    14221 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
    14281 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
    14341 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
    14401 cagtggttca aagttttttt cttccatttc aggtgtcgtg a
//
