LOCUS       sequence-238043-        2146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA 
            aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2146)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C21m
FEATURES             Location/Qualifiers
     source          1..2146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..29
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(33..76)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    79..100
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    168..188
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(194..211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(365..953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(445..464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1124..1915,1916..1984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1747..1766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1985..2089)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
       61 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaaatggg
      121 atcccgactg gcgagagcca ggtaacgaat ggatccaggt agagacctac tagtagcggc
      181 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      361 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1261 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2101 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacatt
//
