LOCUS       sequence-238051-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_FR13_00041321
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(445..461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(445..461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(458..480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    469..485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(493..499,500..517,518..523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    538..559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(676..693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cttggtctct aatggaagat gttccaaaac atggtcaagt tgattctaga catggtcaag
       61 gttcttctaa tcatggtaat aataaaccag ataaacaatt tttgtggggt tggagatgtc
      121 aagttgcttt gattgaacat gttgatggta aaaaagttga agttggtaaa gttgttactg
      181 aaattggtgg tgaaggttct ttgcataatg ttcaattggg tgaaaaaggt tatgttagat
      241 ttaaaactag acaaaattgt tatccagata ttattgaagg tactatggat ggtgatagat
      301 ttgaattggg tcataaaaga agatggactg ttgttagaac tactaaagtt ccaggtgaag
      361 ataaagctcc attggttgct gaaactcaat gagcttagag accaagttcc atcccaatgg
      421 cgcgccgagc ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct
      481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      781 aaagaacatg tgagcaaaag gccagcaaaa ggcc
//
