LOCUS       sequence-238078-        5960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a C. elegan codon optimized florescent protein 
            (CemCardinal2) under the intestinal specific promoter nhx-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Thomas BJ, Wight IE, Chou WYY, Moreno M, Dawson Z, Homayouni A, 
            Huang H, Kim H, Jia H, Buland JR, Wambach JA, Cole FS, Pak SC, 
            Silverman GA, Luke CJ
  TITLE     CemOrange2 fusions facilitate multifluorophore subcellular imaging 
            in C. elegans.
  JOURNAL   PLoS One. 2019 Mar 26;14(3):e0214257. doi: 
            10.1371/journal.pone.0214257. eCollection 2019.
  PUBMED    30913273
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-03-26"; volume: "14(3):e0214257. doi"; pages: " 
            10.1371/journal.pone.0214257. eCollection 2019"
COMMENT     Sequence Label: pJR2953
FEATURES             Location/Qualifiers
     source          1..5960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1753..1767
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(3513..3535)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3635..3654
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3732..3750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3818..3922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3923..3991,3992..4783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4141..4160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4954..5542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5443..5462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5696..5713
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5830..5851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5866..5871,5872..5889,5890..5896)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5904..5920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5909..5931
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5928..5944
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5928..5944
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttccttccaa gttattgaca agtattaact gcacttctca taaaaatttt tcaaatagat
       61 ctatttagtc tggaatagga atacttttgg caattttctt gagaatcata gaagacgaat
      121 ttctgaactg ctctttgtga agccacgccc atatattggc atttttgtat ccaatttttt
      181 gtagagtacc tgaatcattg tcactacatc tgctaaacaa tatatggaag ttctatgaga
      241 ggtgaaaggt aaatcatata acaactgtaa taacatagaa atgattgtag tatatttcca
      301 attaggctga gttgatctta ttgaagagtt tgaatcatag caattttttc ctcttttagt
      361 atttttcaaa acaaattttc acaatgttag atgtagtatt tagcattaag gcatctgtaa
      421 tctcccagaa atgttttggg aataaaaccc tttgactctt tggtcctttt ttgctccaaa
      481 taatatctga ttttaacccg ttctgatcta ctttttacct ttcgatcgtc acatccgcgt
      541 acacgtttct cttgctctca attgcttatc tttatggcct tttcccccac tttttgtcga
      601 ctatcatgcg gacgtggctt ccttgctccc tcccggtttt tttgaaaaca cttgtgaatt
      661 tattttttgt ggtcaaaatt acgaaacatt tgaattctca atctatttat agaccgggtg
      721 tggtatgtta tgccatgttt ttgtcattgc caaatgaaca tcgatatttt aattttctga
      781 acttaccgta ccccctgcgt tactatttta tttttaattt gtataatttc attgctataa
      841 tttgttgtcc ttgaaacatt gaagatatcg aagaaataat gacaaaaatc agatagaaca
      901 atttttccta ctcatcaact cacatttttc tattttctct tgtgcgaaaa aaatagtata
      961 ttgggaatat ttatagtatt gcaacttttc tcttagttcg cagagttgca atactactag
     1021 atccctaata gaggaaatat ggtgaccagc tttttgcaat tggaaagttg ggaaaaaagt
     1081 atatatatta atagacttac attcttgtag ttcgtagaat ttttcaggaa atctgacacg
     1141 caagactttt tcaaaaagtt ttttttttta atttggcaat ctgccaaaac gtttactgaa
     1201 cattttgaaa acatctacca gtacctttaa cacaacgaaa aattgttcta agcaatatta
     1261 actgtgataa ttgatgttaa gacctgaaat ttaatcacaa atttcacctg gaaatgacag
     1321 taatcggctt ctaaatactg tcgacacaag tgccatacct aagattccaa tcctattacc
     1381 atgtttccca atttcattct ctttcgtatc tctatttctc ttctttatta tcaattactt
     1441 tttatcagtt cttcgtttgc tttaacatca agagcacata gcgctctctc gtctctctct
     1501 catgctcttt ttgaactttt acaaaaaact atttccggtt gttttgattc ttggaatttg
     1561 aaataatttt cagtgattaa atctagagga tccccgggat tggccaaagg acccaaaggt
     1621 atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt ggctagcctc
     1681 gagaggcctg gtaccatggg cggaaaaatg gcctccatga ccggaggaca acaaatggga
     1741 cgtgagctgt acgacgacga cgacaaggac ccaatggtct ccaagggaga ggacctcatc
     1801 aaggagaaca tgccaatgaa gctctacatg gagggaaccg tcaacaacca ccacttcaag
     1861 tgcaccaccg agggagaggg aaagccatac gagggaaccc aaacccaacg tatcaaggta
     1921 agcttgaaca tatacatact aactaaccct gattatttaa attttcaggt cgtcgaggga
     1981 ggaccactcc cattcgcctt cgacatcctc gccacctgct tcatgtacgg atccaagacc
     2041 ttcatcaagc acccaaaggt aagtttaaac agttcggtac taactaacca tacatattta
     2101 aattttcagg gaatcccaga cttcttcaag caatccttcc cagagggatt cacctgggag
     2161 cgtgtcacca cctacgagga cggaggagtc ctcaccgtca cccaagacac ctccctccaa
     2221 gacggatgcc tcatctacaa cgtcaagctc cgtggagtca acttcccatc caacggacca
     2281 gtcatgcaaa agaagaccct cggatgggag gccaccaccg agaccctcta cccagccgac
     2341 ggaggactcg agggacgttg cgacatggcc ctcaagctcg acggaggagg acacctccac
     2401 tgcaacctca aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
     2461 cagaccacct accgttccaa gaagccagcc ggaaacctca agatgccagg agtctacttc
     2521 gtcgaccgtc gtctcgagcg tatcaaggag gccgacaacg agacctacgt cgagcaacac
     2581 gaggtcgccg aggcccgtta ctgcgacctc ccatccaagc tcggacacaa gctcaacgga
     2641 atggacgacc tctacaagct taacggtcct aggagatctg aattcggcgc ctaagagctc
     2701 cgcatcggcc gctgtcatca gatcgccatc tcgcgcccgt gcctctgact tctaagtcca
     2761 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     2821 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     2881 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     2941 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     3001 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     3061 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     3121 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     3181 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     3241 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     3301 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     3361 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     3421 gaatacattc ccaatatacc aaacataact gtttctacta gtcggccgta cgggcccttt
     3481 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3541 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3601 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     3661 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3721 cggccttaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3781 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4141 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4441 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5101 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5581 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5641 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5701 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5761 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     5821 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     5881 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     5941 tgaccatgat tacgccaagc
//
