LOCUS       sequence-238239-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_GY11_00121271
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(471..487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(471..487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(484..506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    495..511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(519..525,526..543,544..549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    564..585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(702..719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttggtctcta agctcatctc atatgcaaat caaatctttc ccaatcaact tgaataacat
       61 catcattatg aatacctgga ataacagcaa tttgattttt agaagaataa tgtggtctac
      121 attctctatc aaatctaact ttaacaccaa aaatattttg tttagaattt ttagcagctt
      181 taccatgatg agaaaaagaa taagtacctt cttgagaatg ttttcttctt ctagcatcag
      241 tttcttgatt tctataaaca gtatattcac attcagctgg tggtggagta gaagaagaag
      301 cttttttttt aaaaaaatta acaaaaccag cagcagtatt agaaattcta cctggtggtt
      361 tagttggaac atctggagaa ttagttggaa catctggtgg attagttgga acagcagaca
      421 ttagagacca agttccatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg
      481 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      541 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      601 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      661 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      721 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      781 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
//
