LOCUS       sequence-238273-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_TH16_00119491
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(375..391)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..391)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(388..410)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    399..415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(423..429,430..447,448..453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    468..489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(606..623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(857..876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggtctcta atgcaaattt tttcttctgc tggtgaacca gctactggtc cattgtgttg
       61 taatagaggt gttgttgata cttctggtac ttgtaaatct ttgaatttga atgcttatgc
      121 ttgtgaatct attagatcta attctgctaa agctgttgct ggtgatccag attctaaatc
      181 tggttgtgat aatggtgttt ttgaattgtt tccagttggt agagatgtta aagcttttgt
      241 tccaaattct ggtgatacta ttaaattggg tccatcttct ttgggtgatg cttttactgc
      301 ttttattggt tgtgctgatt gagcttagag accaagttcc atcccaatgg cgcgccgagc
      361 ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      541 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      601 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      661 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      721 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      781 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      841 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      901 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      961 gcgctttctc atagctcacg ctg
//
