LOCUS       sequence-238289-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Golden-Gate compatible vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Petit-Houdenot Y, Langner T, Harant A, Win J, Kamoun S
  TITLE     A Clone Resource of Magnaporthe oryzae Effectors That Share Sequence
            and Structural Similarities Across Host-Specific Lineages.
  JOURNAL   Mol Plant Microbe Interact. 2020 Aug;33(8):1032-1035. doi: 
            10.1094/MPMI-03-20-0052-A. Epub 2020 Jun 22.
  PUBMED    32460610
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-03-20-0052-A"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-08"; volume: "33"; issue: "8"; pages: 
            "1032-1035"
COMMENT     Sequence Label: pUC57-Kan_TH16_ms119_72
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(295..311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(295..311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(308..330)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    319..335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(343..349,350..367,368..373))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    388..409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(526..543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(777..796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttggtctct aagctcaaat atttggacaa ccaaaaacag cagcacaagc agtagcacaa
       61 ttagccatat ctctaacagt aatattagaa aatttcaaag taccataaat accaccagca
      121 acacaaattc tcaaacaagc ccaataatct tcatttcttc tttccaattt tctagaagac
      181 aatttagtat tacatttaac aatatgatct ggatttctag tagaagaatc ttgaatttga
      241 gccattagag accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata
      301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgc
//
