LOCUS       sequence-238374-       13437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses multimeric human VWF protein without A1 domain and Lock A2
            domain. This protein has C-terminal c-Myc tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Zhang C, Kelkar A, Neelamegham S
  TITLE     von Willebrand factor self-association is regulated by the 
            shear-dependent unfolding of the A2 domain.
  JOURNAL   Blood Adv. 2019 Apr 9;3(7):957-968. doi: 
            10.1182/bloodadvances.2018030122.
  PUBMED    30936056
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/bloodadvances"; journalName: "Blood Adv"; date: "2019-04-9-
            9"; volume: "3"; issue: "7"; pages: "957-968"
COMMENT     Sequence Label: pcDNA3.1-deltaA1-Lock-VWF
FEATURES             Location/Qualifiers
     source          1..13437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2540..2551
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8805..8834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8862..8879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8868..9092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9138..9566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9225..9244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9435..9456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9575..9595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9580..9909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9760..9895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9822..9841
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9958..10983
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    11113..11234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11150..11169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11204..11223
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11283..11299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11283..11299)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11296..11318)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11307..11323
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11331..11337,11338..11355,11356..11361))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11376..11397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11514..11531)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11685..12270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11765..11784)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12441..13232,13233..13301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13064..13083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13302..13406)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagctgatcc actagtccag tgtggtggaa ttcccgcagc cctcatttgc
      961 aggggaagat gattcctgcc agatttgccg gggtgctgct tgctctggcc ctcattttgc
     1021 cagggaccct ttgtgcagaa ggaactcgcg gcaggtcatc cacggcccga tgcagccttt
     1081 tcggaagtga cttcgtcaac acctttgatg ggagcatgta cagctttgcg ggatactgca
     1141 gttacctcct ggcagggggc tgccagaaac gctccttctc gattattggg gacttccaga
     1201 atggcaagag agtgagcctc tccgtgtatc ttggggaatt ttttgacatc catttgtttg
     1261 tcaatggtac cgtgacacag ggggaccaaa gagtctccat gccctatgcc tccaaagggc
     1321 tgtatctaga aactgaggct gggtactaca agctgtccgg tgaggcctat ggctttgtgg
     1381 ccaggatcga tggcagcggc aactttcaag tcctgctgtc agacagatac ttcaacaaga
     1441 cctgcgggct gtgtggcaac tttaacatct ttgctgaaga tgactttatg acccaagaag
     1501 ggaccttgac ctcggaccct tatgactttg ccaactcatg ggctctgagc agtggagaac
     1561 agtggtgtga acgggcatct cctcccagca gctcatgcaa catctcctct ggggaaatgc
     1621 agaagggcct gtgggagcag tgccagcttc tgaagagcac ctcggtgttt gcccgctgcc
     1681 accctctggt ggaccccgag ccttttgtgg ccctgtgtga gaagactttg tgtgagtgtg
     1741 ctggggggct ggagtgcgcc tgccctgccc tcctggagta cgcccggacc tgtgcccagg
     1801 agggaatggt gctgtacggc tggaccgacc acagcgcgtg cagcccagtg tgccctgctg
     1861 gtatggagta taggcagtgt gtgtcccctt gcgccaggac ctgccagagc ctgcacatca
     1921 atgaaatgtg tcaggagcga tgcgtggatg gctgcagctg ccctgaggga cagctcctgg
     1981 atgaaggcct ctgcgtggag agcaccgagt gtccctgcgt gcattccgga aagcgctacc
     2041 ctcccggcac ctccctctct cgagactgca acacctgcat ttgccgaaac agccagtgga
     2101 tctgcagcaa tgaagaatgt ccaggggagt gccttgtcac tggtcaatcc cacttcaaga
     2161 gctttgacaa cagatacttc accttcagtg ggatctgcca gtacctgctg gcccgggatt
     2221 gccaggacca ctccttctcc attgtcattg agactgtcca gtgtgctgat gaccgcgacg
     2281 ctgtgtgcac ccgctccgtc accgtccggc tgcctggcct gcacaacagc cttgtgaaac
     2341 tgaagcatgg ggcaggagtt gccatggatg gccaggacat ccagctcccc ctcctgaaag
     2401 gtgacctccg catccagcat acagtgacgg cctccgtgcg cctcagctac ggggaggacc
     2461 tgcagatgga ctgggatggc cgcgggaggc tgctggtgaa gctgtccccc gtctacgccg
     2521 ggaagacctg cggcctgtgt gggaattaca atggcaacca gggcgacgac ttccttaccc
     2581 cctctgggct ggcagagccc cgggtggagg acttcgggaa cgcctggaag ctgcacgggg
     2641 actgccagga cctgcagaag cagcacagcg atccctgcgc cctcaacccg cgcatgacca
     2701 ggttctccga ggaggcgtgc gcggtcctga cgtcccccac attcgaggcc tgccatcgtg
     2761 ccgtcagccc gctgccctac ctgcggaact gccgctacga cgtgtgctcc tgctcggacg
     2821 gccgcgagtg cctgtgcggc gccctggcca gctatgccgc ggcctgcgcg gggagaggcg
     2881 tgcgcgtcgc gtggcgcgag ccaggccgct gtgagctgaa ctgcccgaaa ggccaggtgt
     2941 acctgcagtg cgggaccccc tgcaacctga cctgccgctc tctctcttac ccggatgagg
     3001 aatgcaatga ggcctgcctg gagggctgct tctgcccccc agggctctac atggatgaga
     3061 ggggggactg cgtgcccaag gcccagtgcc cctgttacta tgacggtgag atcttccagc
     3121 cagaagacat cttctcagac catcacacca tgtgctactg tgaggatggc ttcatgcact
     3181 gtaccatgag tggagtcccc ggaagcttgc tgcctgacgc tgtcctcagc agtcccctgt
     3241 ctcatcgcag caaaaggagc ctatcctgtc ggccccccat ggtcaagctg gtgtgtcccg
     3301 ctgacaacct gcgggctgaa gggctcgagt gtaccaaaac gtgccagaac tatgacctgg
     3361 agtgcatgag catgggctgt gtctctggct gcctctgccc cccgggcatg gtccggcatg
     3421 agaacagatg tgtggccctg gaaaggtgtc cctgcttcca tcagggcaag gagtatgccc
     3481 ctggagaaac agtgaagatt ggctgcaaca cttgtgtctg tcgggaccgg aagtggaact
     3541 gcacagacca tgtgtgtgat gccacgtgct ccacgatcgg catggcccac tacctcacct
     3601 tcgacgggct caaatacctg ttccccgggg agtgccagta cgttctggtg caggattact
     3661 gcggcagtaa ccctgggacc tttcggatcc tagtggggaa taagggatgc agccacccct
     3721 cagtgaaatg caagaaacgg gtcaccatcc tggtggaggg aggagagatt gagctgtttg
     3781 acggggaggt gaatgtgaag aggcccatga aggatgagac tcactttgag gtggtggagt
     3841 ctggccggta catcattctg ctgctgggca aagccctctc cgtggtctgg gaccgccacc
     3901 tgagcatctc cgtggtcctg aagcagacat accaggagaa agtgtgtggc ctgtgtggga
     3961 attttgatgg catccagaac aatgacctca ccagcagcaa cctccaagtg gaggaagacc
     4021 ctgtggactt tgggaactcc tggaaagtga gctcgcagtg tgctgacacc agaaaagtgc
     4081 ctctggactc atcccctgcc acctgccata acaacatcat gaagcagacg atggtggatt
     4141 cctcctgtag aatccttacc agtgacgtct tccaggactg caacaagctg gtggaccccg
     4201 agccatatct ggatgtctgc atttacgaca cctgctcctg tgagtccatt ggggactgcg
     4261 cctgcttctg cgacaccatt gctgcctatg cccacgtgtg tgcccagcat ggcaaggtgg
     4321 tgacctggag gacggccaca ttgtgccccc agagctgcga ggagaggaat ctccgggaga
     4381 acgggtatga gtgtgagtgg cgctataaca gctgtgcacc tgcctgtcaa gtcacgtgtc
     4441 agcaccctga gccactggcc tgccctgtgc agtgtgtgga gggctgccat gcccactgcc
     4501 ctccagggaa aatcctggat gagcttttgc agacctgcgt tgaccctgaa gactgtccag
     4561 tgtgtgaggt ggctggccgg cgttttgcct caggaaagaa agtcaccttg aatcccagtg
     4621 accctgagca ctgccagatt tgccactgtg atgttgtcaa cctcacctgt gaagcctgcc
     4681 aggagccggg aggcctggtg gtgcctccca cagatgcccc ggtgagcccc accactctgt
     4741 atgtggagga catcttcgaa cccccccaca tggcacaagt taacgtaggt ccggggctct
     4801 tgggggtttc gaccctgggg cccaagaggt gctccatggt tctggatgtg gcgttcgtcc
     4861 tggaaggatc ggacaaaatt ggtgaagccg acttcaacag gagcaaggag ttcatggagg
     4921 aggtgattca gcggatggat gtgggccagg acagcatcca cgtcacggtg ctgcagtact
     4981 cctacatggt gaccgtggag taccccttca gcgaggcaca gtccaaaggg gacatcctgc
     5041 agcgggtgcg agagatccgc taccagggcg gcaacaggac caacactggg ctggccctgc
     5101 ggtacctctc tgaccacagc ttcttggtca gccagggtga ccgggagcag gcgcccaacc
     5161 tggtctacat ggtcaccgga aatcctgcct ctgatgagat caagaggctg cctggagaca
     5221 tccaggtggt gcccattgga gtgggcccta atgccaacgt gcaggagctg gagaggattg
     5281 gctggcccaa tgcccctatc ctcatccagg actttgagac gctcccccga gaggctcctg
     5341 acctggtgct gcagagtggt tgtaccggtg aggggctgca gatccccacc ctctcccctg
     5401 cacctgactg cagccagccc ctggacgtga tccttctcct ggatggctcc tccagtttcc
     5461 cagcttctta ttttgatgaa atgaagagtt tcgccaaggc tttcatttca aaagccaata
     5521 tagggcctcg tctcactcag gtgtcagtgc tgcagtatgg aagcatcacc accattgacg
     5581 tgccatggaa cgtggtcccg gagaaagccc atttgctgag ccttgtggac gtcatgcagc
     5641 gggagggagg ccccagccaa atcggggatg ccttgggctt tgctgtgcga tacttgactt
     5701 cagaaatgca tggtgccagg ccgggagcct caaaggcggt ggtcatcctg gtcacggacg
     5761 tctctgtgga ttcagtggat gcagcagctg atgccgccag gtccaacaga gtgacagtgt
     5821 tccctattgg aattggagat cgctacgatg cagcccagct acggatcttg gcaggcccag
     5881 caggcgactc caacgtggtg aagctccagc gaatcgaaga cctccctacc atggtcacct
     5941 tgggcaattc cttcctccac aaactgtgct ctggatttgt taggatttgc atggatgagg
     6001 atgggaatga gaagaggccc ggggacgtct ggaccttgcc agaccagtgc cacaccgtga
     6061 cttgccagcc agatggccag accttgctga agagtcatcg ggtcaactgt gaccgggggc
     6121 tgaggccttc gtgccctaac agccagtccc ctgttaaagt ggaagagacc tgtggctgcc
     6181 gctggacctg cccctgcgtg tgcacaggca gctccactcg gcacatcgtg acctttgatg
     6241 ggcagaattt caagctgact ggcagctgtt cttatgtcct atttcaaaac aaggagcagg
     6301 acctggaggt gattctccat aatggtgcct gcagccctgg agcaaggcag ggctgcatga
     6361 aatccatcga ggtgaagcac agtgccctct ccgtcgagct gcacagtgac atggaggtga
     6421 cggtgaatgg gagactggtc tctgttcctt acgtgggtgg gaacatggaa gtcaacgttt
     6481 atggtgccat catgcatgag gtcagattca atcaccttgg tcacatcttc acattcactc
     6541 cacaaaacaa tgagttccaa ctgcagctca gccccaagac ttttgcttca aagacgtatg
     6601 gtctgtgtgg gatctgtgat gagaacggag ccaatgactt catgctgagg gatggcacag
     6661 tcaccacaga ctggaaaaca cttgttcagg aatggactgt gcagcggcca gggcagacgt
     6721 gccagcccat cctggaggag cagtgtcttg tccccgacag ctcccactgc caggtcctcc
     6781 tcttaccact gtttgctgaa tgccacaagg tcctggctcc agccacattc tatgccatct
     6841 gccagcagga cagttgccac caggagcaag tgtgtgaggt gatcgcctct tatgcccacc
     6901 tctgtcggac caacggggtc tgcgttgact ggaggacacc tgatttctgt gctatgtcat
     6961 gcccaccatc tctggtctac aaccactgtg agcatggctg tccccggcac tgtgatggca
     7021 acgtgagctc ctgtggggac catccctccg aaggctgttt ctgccctcca gataaagtca
     7081 tgttggaagg cagctgtgtc cctgaagagg cctgcactca gtgcattggt gaggatggag
     7141 tccagcacca gttcctggaa gcctgggtcc cggaccacca gccctgtcag atctgcacat
     7201 gcctcagcgg gcggaaggtc aactgcacaa cgcagccctg ccccacggcc aaagctccca
     7261 cgtgtggcct gtgtgaagta gcccgcctcc gccagaatgc agaccagtgc tgccccgagt
     7321 atgagtgtgt gtgtgaccca gtgagctgtg acctgccccc agtgcctcac tgtgaacgtg
     7381 gcctccagcc cacactgacc aaccctggcg agtgcagacc caacttcacc tgcgcctgca
     7441 ggaaggagga gtgcaaaaga gtgtccccac cctcctgccc cccgcaccgt ttgcccaccc
     7501 ttcggaagac ccagtgctgt gatgagtatg agtgtgcctg caactgtgtc aactccacag
     7561 tgagctgtcc ccttgggtac ttggcctcaa ccgccaccaa tgactgtggc tgtaccacaa
     7621 ccacctgcct tcccgacaag gtgtgtgtcc accgaagcac catctaccct gtgggccagt
     7681 tctgggagga gggctgcgat gtgtgcacct gcaccgacat ggaggatgcc gtgatgggcc
     7741 tccgcgtggc ccagtgctcc cagaagccct gtgaggacag ctgtcggtcg ggcttcactt
     7801 acgttctgca tgaaggcgag tgctgtggaa ggtgcctgcc atctgcctgt gaggtggtga
     7861 ctggctcacc gcggggggac tcccagtctt cctggaagag tgtcggctcc cagtgggcct
     7921 ccccggagaa cccctgcctc atcaatgagt gtgtccgagt gaaggaggag gtctttatac
     7981 aacaaaggaa cgtctcctgc ccccagctgg aggtccctgt ctgcccctcg ggctttcagc
     8041 tgagctgtaa gacctcagcg tgctgcccaa gctgtcgctg tgagcgcatg gaggcctgca
     8101 tgctcaatgg cactgtcatt gggcccggga agactgtgat gatcgatgtg tgcacgacct
     8161 gccgctgcat ggtgcaggtg ggggtcatct ctggattcaa gctggagtgc aggaagacca
     8221 cctgcaaccc ctgccccctg ggttacaagg aagaaaataa cacaggtgaa tgttgtggga
     8281 gatgtttgcc tacggcttgc accattcagc taagaggagg acagatcatg acactgaagc
     8341 gtgatgagac gctccaggat ggctgtgata ctcacttctg caaggtcaat gagagaggag
     8401 agtacttctg ggagaagagg gtcacaggct gcccaccctt tgatgaacac aagtgtctgg
     8461 ctgagggagg taaaattatg aaaattccag gcacctgctg tgacacatgt gaggagcctg
     8521 agtgcaacga catcactgcc aggctgcagt atgtcaaggt gggaagctgt aagtctgaag
     8581 tagaggtgga tattcactac tgccagggca aatgtgccag caaagccatg tactccattg
     8641 acatcaacga tgtgcaggac cagtgctcct gctgctctcc gacacggacg gagcccatgc
     8701 aggtggccct gcactgcacc aatggctctg ttgtgtacca tgaggttctc aatgccatgg
     8761 agtgcaaatg ctcccccagg aagtgcagca aggatatcgc tagcgaacag aagctaataa
     8821 gtgaagagga cctaatctga gaattcaaac ccgctgatca gcctcgactg tgccttctag
     8881 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8941 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     9001 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     9061 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     9121 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     9181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     9241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     9301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     9361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     9421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     9481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     9541 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     9601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9661 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9841 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9901 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
     9961 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
    10021 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
    10081 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
    10141 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
    10201 gagttcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
    10261 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
    10321 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
    10381 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
    10441 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
    10501 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
    10561 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
    10621 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
    10681 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
    10741 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
    10801 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
    10861 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
    10921 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
    10981 tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
    11041 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
    11101 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    11161 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    11221 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
    11281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    11341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
    12241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    12301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    12361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    12421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    12481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    12541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    12601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    12661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    12721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    12781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    13021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    13081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    13141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    13201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    13261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    13321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    13381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
