LOCUS       sequence-23845-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein 
            M, Ehrt S, Schnappinger D
  TITLE     Silencing Mycobacterium smegmatis by using tetracycline repressors.
  JOURNAL   J Bacteriol. 2007 Jul . 189(13):4614-23.
  PUBMED    17483222
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2007 Jul . 189(13):4614-23."
COMMENT     Sequence Label: pTEK-4S-0X
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     667..686
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 tgatattgat ttaatactag tgttactatt taattgggga ccctagaggt cccctttttt
       61 attttaaaaa ttttttcaag gggacctcta gggtccccag ctggctagcg agtcatgagg
      121 tcaccatggc ctcagactat ttgcaacagt gccgtaagct gcacctcaaa cccccggatc
      181 aggctctcca ggccatgcag aaaggcctgc tcaccatcat cactgtccat aatctgcagc
      241 gcttcccgca atagcggcgg caggttttcg tccggtgctg cagggcggtc ggtcagggcg
      301 gcagtatgct cctgctgctc cagtacggca cctaaggtaa aatgactgac cgctgaaatc
      361 gcatataacc cgtcgcgcag tgaaaagccg ttttctgtca taaagcgtaa ctgggtttcc
      421 accgtatcat actgtttttc atcaggacgc gtgccgaggt gcacttttgc cccgtcacgg
      481 taacgcagca gcgcgcggcg gaaactcatt gcattattgc gcagaaatga ctgccaggat
      541 tcccccgccg caggcagtga ataatcatga tgacgcgcca agatctccac cgccagcgca
      601 tccagtagcn tttntttttt tttttacatg ccaatanaat ngaggctgct ctacaccaag
      661 cttctgggcg agtttacggg ttgttacacc ttcgattccg acctcattac ccagcgccaa
      721 tgcgctgtta atcactttac ttttatctaa tctagacatt gcgantgatc ctcctgatgt
      781 accgcgaacg gctcattcgg cgcgatctct cgcgngaatg atg
//
