LOCUS       sequence-238692-       13968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Kweon SM, Cho YJ, Minoo P, Groffen J, Heisterkamp N
  TITLE     Activity of the Bcr GTPase-activating domain is regulated through 
            direct protein/protein interaction with the Rho guanine nucleotide 
            dissociation inhibitor.
  JOURNAL   J Biol Chem. 2008 Feb 8;283(6):3023-30. Epub 2007 Dec 10.
  PUBMED    18070886
REFERENCE   2  (bases 1 to 13968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-02-8"; volume: "283(6)"; pages: "3023-30. Epub 
            2007 Dec 10"
COMMENT     Sequence Label: Bcr/Abl P190-pLEF
FEATURES             Location/Qualifiers
     source          1..13968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             911..1564
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     1522..1544
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1571..1588
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        complement(8228..8246)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8229..8246)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8266..8283)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8272..8496
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8542..8970
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8984..9313
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9164..9299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9380..10174
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9434..9453)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10044..10063
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10348..10469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10385..10404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10439..10458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10518..10534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10518..10534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10531..10553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10542..10558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10566..10572,10573..10590,10591..10596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10611..10632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10749..10766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10920..11508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11000..11019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11679..12470,12471..12539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12302..12321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12540..12644)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12719..12738)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(13212..13667)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     13793..13815
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13807..13824
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13808..13824
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttagcttacc atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
      961 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
     1021 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
     1081 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
     1141 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
     1201 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
     1261 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
     1321 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
     1381 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
     1441 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
     1501 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
     1561 aaaatcggat ctggttccgc gtggatcccc cgggctgcag gaattcgatg gccgcgccat
     1621 ggtggacccg gtgggcttcg cggaggcgtg gaaggcgcag ttcccggact cagagccccc
     1681 gcgcatggag ctgcgctcag tgggcgacat cgagcaggag ctggagcgct gcaaggcctc
     1741 cattcggcgc ctggagcagg aggtgaacca ggagcgcttc cgcatgatct acctgcagac
     1801 gttgctggcc aaggaaaaga agagctatga ccggcagcga tggggcttcc ggcgcgcggc
     1861 gcaggccccc gacggcgcct ccgagccccg agcgtccgcg tcgcgcccgc agccagcgcc
     1921 cgccgacgga gccgacccgc cgcccgccga ggagcccgag gcccggcccg acggcgaggg
     1981 ttctccgggt aaggccaggc ccgggaccgc ccgcaggccc ggggcagccg cgtcggggga
     2041 acgggacgac cggggacccc ccgccagcgt ggcggcgctc aggtccaact tcgagcggat
     2101 acgcaagggc catggccagc ccggggcgga cgccgagaag cccttctacg tgaacgtcga
     2161 gtttcaccac gagcgcggcc tggtgaaggt caacgacaaa gaggtgtcgg accgcatcag
     2221 ctccctgggc agccaggcca tgcagatgga gcgcaaaaag tcccagcacg gcgcgggctc
     2281 gagcgtgggg gatgcatcca ggccccctta ccggggacgc tcctcggaga gcagctgcgg
     2341 cgtcgacggc gactacgagg acgccgagtt gaacccccgc ttcctgaagg acaacctgat
     2401 cgacgccaat ggcggtagca ggcccccttg gccgcccctg gagtaccagc cctaccagag
     2461 catctacgtc gggggcatga tggaagggga gggcaagggc ccgctcctgc gcagccagag
     2521 cacctctgag caggagaagc gccttacctg gccccgcagg tcctactccc cccggagttt
     2581 tgaggattgc ggaggcggct ataccccgga ctgcagctcc aatgagaacc tcacctccag
     2641 cgaggaggac ttctcctctg gccagtccag ccgcgtgtcc ccaagcccca ccacctaccg
     2701 catgttccgg gacaaaagcc gctctccctc gcagaactcg caacagtcct tcgacagcag
     2761 cagtcccccc acgccgcagt gccataagcg gcaccggcac tgcccggttg tcgtgtccga
     2821 ggccaccatc gtgggcgtcc gcaagaccgg gcagatctgg cccaacgatg gcgagggcgc
     2881 cttccatgga gacgcagaag cccttcagcg gccagtagca tctgactttg agcctcaggg
     2941 tctgagtgaa gccgctcgtt ggaactccaa ggaaaacctt ctcgctggac ccagtgaaaa
     3001 tgaccccaac cttttcgttg cactgtatga ttttgtggcc agtggagata acactctaag
     3061 cataactaaa ggtgaaaagc tccgggtctt aggctataat cacaatgggg aatggtgtga
     3121 agcccaaacc aaaaatggcc aaggctgggt cccaagcaac tacatcatgc cagtcaacag
     3181 tctggagaaa cactcctggt accatgggcc tgtgtcccgc aatgccgctg agtatctgct
     3241 gagcagcggg atcaatggca gcttcttggt gcgtgagagt gagagcagtc ctggccagag
     3301 gtccatctcg ctgagatacg aagggagggt gtaccattac aggatcaaca ctgcttctga
     3361 tggcaagctc tacgtctcct ccgagagccg cttcaacacc ctggccgagt tggttcatca
     3421 tcattcaacg gtggccgacg ggctcatcac cacgctccat tatccagccc caaagcgcaa
     3481 caagcccact gtctatggtg tgtcccccaa ctacgacaag tgggagatgg aacgcacgga
     3541 catcaccatg aagcacaagc tgggcggggg ccagtacggg gaggtgtacg agggcgtgtg
     3601 gaagaaatac agcctgacgg tggccgtgaa gaccttgaag gaggacacca tggaggtgga
     3661 agagttcttg aaagaagctg cagtcatgaa agagatcaaa caccctaacc tggtgcagct
     3721 ccttggggtc tgcacccggg agcccccgtt ctatatcatc actgagttca tgacctacgg
     3781 gaacctcctg gactacctga gggagtgcaa ccggcaggag gtgaacgccg tggtgctgct
     3841 gtacatggcc actcagatct cgtcagccat ggagtacctg gagaagaaaa acttcatcca
     3901 cagagatctt gctgcccgaa actgcctggt aggggagaac cacttggtga aggtagctga
     3961 ttttggcctg agcaggttga tgacagggga cacctacaca gcccatgctg gagccaagtt
     4021 ccccatcaaa tggactgcac ccgagagcct ggcctacaac aagttctcca tcaagtccga
     4081 cgtctgggca tttggagtat tgctttggga aattgctacc tatggcatgt ccccttaccc
     4141 gggaattgac ctgtcccagg tgtatgagct gctagagaag gactaccgca tggagcgccc
     4201 agaaggctgc ccagagaagg tctatgaact catgcgagca tgttggcagt ggaatccctc
     4261 tgaccggccc tcctttgctg aaatccacca agcctttgaa acaatgttcc aggaatccag
     4321 tatctcagac gaagtggaaa aggagctggg gaaacaaggc gtccgtgggg ctgtgagtac
     4381 cttgctgcag gccccagagc tgcccaccaa gacgaggacc tccaggagag ctgcagagca
     4441 cagagacacc actgacgtgc ctgagatgcc tcactccaag ggccagggag agagcgatcc
     4501 tctggaccat gagcctgccg tgtctccatt gctccctcga aaagagcgag gtcccccgga
     4561 gggcggcctg aatgaagatg agcgccttct ccccaaagac aaaaagacca acttgttcag
     4621 cgccttgatc aagaagaaga agaagacagc cccaacccct cccaaacgca gcagctcctt
     4681 ccgggagatg gacggccagc cggagcgcag aggggccggc gaggaagagg gccgagacat
     4741 cagcaacggg gcactggctt tcaccccctt ggacacagct gacccagcca agtccccaaa
     4801 gcccagcaat ggggctgggg tccccaatgg agccctccgg gagtccgggg gctcaggctt
     4861 ccggtctccc cacctgtgga agaagtccag cacgctgacc agcagccgcc tagccaccgg
     4921 cgaggaggag ggcggtggca gctccagcaa gcgcttcctg cgctcttgct ccgcctcctg
     4981 cgttccccat ggggccaagg acacggagtg gaggtcagtc acgctgcctc gggacttgca
     5041 gtccacggga agacagtttg actcgtccac atttggaggg cacaaaagtg agaagccggc
     5101 tctgcctcgg aagagggcag gggagaacag gtctgaccag gtgacccgag gcacagtaac
     5161 gcctcccccc aggctggtga aaaagaatga ggaagctgct gatgaggtct tcaaagacat
     5221 catggagtcc agcccgggct ccagcccgcc caacctgact ccaaaacccc tccggcggca
     5281 ggtcaccgtg gcccctgcct cgggcctccc ccacaaggaa gaagctggaa agggcagtgc
     5341 cttagggacc cctgctgcag ctgagccagt gacccccacc agcaaagcag gctcaggtgc
     5401 accagggggc accagcaagg gccccgccga ggagtccaga gtgaggaggc acaagcactc
     5461 ctctgagtcg ccagggaggg acaaggggaa attgtccagg ctcaaacctg ccccgccgcc
     5521 cccaccagca gcctctgcag ggaaggctgg aggaaagccc tcgcagagcc cgagccagga
     5581 ggcggccggg gaggcagtcc tgggcgcaaa gacaaaagcc acgagtctgg ttgatgctgt
     5641 gaacagtgac gctgccaagc ccagccagcc gggagagggc ctcaaaaagc ccgtgctccc
     5701 ggccactcca aagccacagt ccgccaagcc gtcggggacc cccatcagcc cagcccccgt
     5761 tccctccacg ttgccatcag catcctcggc cctggcaggg gaccagccgt cttccaccgc
     5821 cttcatccct ctcatatcaa cccgagtgtc tcttcggaaa acccgccagc ctccagagcg
     5881 gatcgccagc ggcgccatca ccaagggcgt ggtcctggac agcaccgagg cgctgtgcct
     5941 cgccatctct aggaactccg agcagatggc cagccacagc gcagtgctgg aggccggcaa
     6001 aaacctctac acgttctgcg tgagctatgt ggattccatc cagcaaatga ggaacaagtt
     6061 tgccttccga gaggccatca acaaactgga gaataatctc cgggagcttc agatctgccc
     6121 ggcgacagca ggcagtggtc cagcggccac tcaggacttc agcaagctcc tcagttcggt
     6181 gaaggaaatc agtgacatag tgcagaggta gcagcagtca ggggtcaggt gtcaggcccg
     6241 tcggagctgc ctgcagcaca tgcgggctcg cccatacccg tgacagtggc tgacaaggga
     6301 ctagtgagtc agcaccttgg cccaggagct ctgcgccagg cagagctgag ggccctgtgg
     6361 agtccagctc tactacctac gtttgcaccg cctgccctcc cgcaccttcc tcctccccgc
     6421 tccgtctctg tcctcgaatt ttatctgtgg agttcctgct ccgtggactg cagtcggcat
     6481 gccaggaccc gccagccccg ctcccaccta gtgccccaga ctgagctctc caggccaggt
     6541 gggaacggct gatgtggact gtctttttca tttttttctc tctggagccc ctcctccccc
     6601 ggctgggcct ccttcttcca cttctccaag aatggaagcc tgaactgagg ccttgtgtgt
     6661 caggccctct gcctgcactc cctggccttg cccgtcgtgt gctgaagaca tgtttcaaga
     6721 accgcatttc gggaagggca tgcacgggca tgcacacggc tggtcactct gccctctgct
     6781 gctgcccggg gtggggtgca ctcgccattt cctcacgtgc aggacagctc ttgatttggg
     6841 tggaaaacag ggtgctaaag ccaaccagcc tttgggtcct gggcaggtgg gagctgaaaa
     6901 ggatcgaggc atggggcatg tcctttccat ctgtccacat ccccagagcc cagctcttgc
     6961 tctcttgtga cgtgcactgt gaatcctggc aagaaagctt gagtctcaag ggtggcaggt
     7021 cactgtcact gccgacatcc ctcccccagc agaatggagg caggggacaa gggaggcagt
     7081 ggctagtggg gtgaacagct ggtgccaaat agccccagac tgggcccagg caggtctgca
     7141 agggcccaga gtgaaccgtc ctttcacaca tctgggtgcc ctgaaagggc ccttcccctc
     7201 ccccactcct ctaagacaaa gtagattctt acaaggccct ttcctttgga acaagacagc
     7261 cttcactttt ctgagttctt gaagcatttc aaagccctgc ctctgtgtag ccgccctgag
     7321 agagaataga gctgccactg ggcacctgcg cacaggtggg aggaaagggc ctggccagtc
     7381 ctggtcctgg ctgcactctt gaactgggcg aatgtcttat ttaattaccg tgagtgacat
     7441 agcctcatgt tctgtggggg tcatcaggga gggttaggaa aaccacaaac ggagcccctg
     7501 aaagcctcac gtatttcaca gagcacgcct gccatcttct ccccgaggct gccccaggcc
     7561 ggagcccaga tacgggggct gtgactctgg gcagggaccc ggggtctcct ggaccttgac
     7621 agagcagcta actccgagag cagtgggcag gtggccgccc ctgaggcttc acgccgggag
     7681 aagccacctt cccacccctt cataccgcct cgtgccagca gcctcgcaca ggccctagct
     7741 ttacgctcat cacctaaact tgtactttat ttttctgata gaaatggttt cctctggatc
     7801 gttttatgcg gttcttacag cacatcacct ctttgccccc gacggctgtg acgcagccgg
     7861 agggaggcac tagtcaccga cagcggcctt gaagacagag caaagcgccc acccaggtcc
     7921 cccgactgcc tgtctccatg aggtactggt cccttccttt tgttaacgtg atgtgccact
     7981 atattttaca cgtatctctt ggtatgcatc ttttatagac gctcttttct aagtggcgtg
     8041 tgcatagcgt cctgccctgc cccctcgggg gcctgtggtg gctccccctc tgcttctcgg
     8101 ggtccagtgc attttgtttc tgtatatgat tctctgtggt tttttttgaa tccaaatctg
     8161 tcctctgtag tattttttaa ataaatcagt gtttacatta gaattccccg ggtctagagg
     8221 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     8281 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8341 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8401 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8461 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8521 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8941 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9061 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9121 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9241 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9301 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     9361 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     9421 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     9481 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     9541 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     9601 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     9661 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     9721 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     9781 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     9841 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     9901 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     9961 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
    10021 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
    10081 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
    10141 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
    10201 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
    10261 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10321 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10441 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10501 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10561 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10621 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10741 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10801 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10861 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11581 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11821 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    12001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    12301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12661 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
    12721 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    12781 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    12841 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
    12901 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
    12961 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
    13021 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    13081 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    13141 cagtacatca agtgtatcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    13201 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    13261 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    13321 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    13381 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    13441 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    13501 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    13561 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    13621 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
    13681 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    13741 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    13801 cgacgttgta aaacgacggc cagtgaattg tcgacttctg aggcggaaag aaccagctgt
    13861 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    13921 aaagcatgca tctcaattag tcagcaagga aagtccccag gctcccca
//
