LOCUS       sequence-23874-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w2-Prd
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             217..486
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             496..519
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catncntacg cgcaatgcaa cctcccaatt caagtgcgga caacaaccct ttgcaacagc
       61 aatcatcaca aaataccgta ccaaacgtcc tcaaccaaat taaccaaatc ttttctccag
      121 aggagcaacg cagcttatta caagaagcca tcgaaacctg caagaatttt gaaaaaacac
      181 aattgtaagt ctcggctcca agaaggagat atacccatgg cacgcgtaac tgttcaggac
      241 gctgtagaga aaattggtaa ccgttttgac ctggtactgg tcgccgcgcg tcgcgctcgt
      301 cagatgcagg taggcggaaa ggacccgctc gtaccggaag aaaacgataa aaccactgta
      361 atcgcgctgc gcgaaatcga agaaggtctg atcaacaacc agatcctcga cgttcgcgaa
      421 cgccaggaac agcaagagca ggaagccgct gaattacaag ccgttaccgc tattgctgaa
      481 ggtcgtgcgg ccgcggacta caaggatgac gacgacaagt tccggaccgg ttccaagaca
      541 cccccccatg gtaccaacag cggccagggg cgcgtcaatc aactaggtgg agttttcatc
      601 aacggtcgtc ctttgcccaa caatattcgt cttaaaatcg tcgagnngcn nnnanattgg
      661 cnttcggccc ttgtgttgtc tccagacagc tacgtgtgtc ccatggctgc gtatcgaaga
      721 tcctgaatcg ctaccaggag actggctcca ttagaccagg cgtaattggt ggctccaagc
      781 cgaggatagc cacgcccgaa tcgagaccga ntgaggagta cagcgca
//
