LOCUS       sequence-239153-       21553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Abscisic acid-inducible FUT8 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21553)
  AUTHORS   Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, 
            Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, 
            Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B 
            Jr, Summers NM, Lu TK, Weiss R
  TITLE     Small-molecule control of antibody N-glycosylation in engineered 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4.
  PUBMED    31110306
REFERENCE   2  (bases 1 to 21553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4."
COMMENT     Sequence Label: FUT8-ABA
FEATURES             Location/Qualifiers
     source          1..21553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    26..46
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    64..101
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..549
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(551..575)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    730..785
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    1215..1240
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    1912..1932
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     2185..2209
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2226..2250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3989..4013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4168..4223
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4584..4600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4608..4624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4632..4638,4639..4656,4657..4662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4677..4698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4822..4847
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    5519..5539
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5583..5794
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5795..6733
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    6758..6782
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6798..6818
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6819..7052
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             7593..7619
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(7630..7654)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7809..7864
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8225..8241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8249..8265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8273..8279,8280..8297,8298..8303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8318..8339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8463..8488
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    9160..9180
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        9224..9435
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          9436..10374
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    10399..10423
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      10457..10466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12008..12031
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(12060..12084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    12239..12294
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(12655..12671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12679..12695
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12703..12709,12710..12727,12728..12733))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12748..12769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12893..12918
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    13590..13610
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        13654..13865
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          13866..14804
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    14829..14853
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      14858..14867
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14864..15577
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(15579..15603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    15758..15813
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(16174..16190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16198..16214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16222..16228,16229..16246,16247..16252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16267..16288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    17122..17958
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(18069..18088)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             18080..18889
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(18147..18166)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(19142..19730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19222..19241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19901..20692,20693..20761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     20524..20543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20762..20866)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(20892..21347)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(21029..21050)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     21241..21260
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     21474..21496
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     21488..21505
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     21489..21505
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     21515..21534
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21515..21533
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtcgacgt taacgctagt gatatcaact ttgtatagaa aagttggctc cgaatttctc
       61 gagggcttgt cgacgacggc ggtctccgtc gtcaggatca tgaattcgac ccaagtttgt
      121 acaaaaaagc aggctgaacg gcctgccacc atggccaagc ctttgtctca agaagaatcc
      181 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      241 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      301 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      361 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      421 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      481 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
      541 gagggctaat acccagcttt cttgtacaaa gtggtacgcg tgaattcact cctcaggtgc
      601 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
      661 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
      721 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
      781 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
      841 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
      901 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
      961 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     1021 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     1081 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttgaattct
     1141 ctagaattac cctgttatcc ctaggtttac cgagctctta ttggttttca aacttcattg
     1201 actgtgccaa gcttctgacc tcttctcttc ctcccacagg gcctcgagac aattgcatcg
     1261 atggtaccag tgaaagtctg caatgaattc gagttggcgc gcctgtcatt ctaaatctct
     1321 ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     1381 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     1441 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     1501 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggagggggc
     1561 tgtccccgtg agctcccagg cgcgcctgtc attctaaatc tctctttcag cctaaagctt
     1621 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     1681 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     1741 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     1801 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtcccc gtgagctccc
     1861 aggcgcgcca actcggtacc gtatcgatgt cgacgttaac gctagtgata tcaactttgt
     1921 atagaaaagt tggctgcggc cgcgaaatga tacgaaacgt accgtatcgt taaggtgaat
     1981 gatacgaaac gtaccgtatc gttaaggtca atgatacgaa acgtaccgta tcgttaaggt
     2041 caatgatacg aaacgtaccg tatcgttaag gtgtatgata cgaaacgtac cgtatcgtta
     2101 aggtagatga tacgaaacgt accgtatcgt taaggtcggg tctcagctag ctaggcgtgt
     2161 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg ggtctcggaa
     2221 ttcgaccaag tttgtacaaa aaagcaggct gatgccacca tgcgggcatg gactggttcc
     2281 tggcgttgga ttatgctcat tctttttgcc tgggggacct tattgtttta tataggtggt
     2341 catttggttc gagataatga ccaccctgac cattctagca gagaactctc caagattctt
     2401 gcaaagctgg agcgcttaaa acaacaaaat gaagacttga ggagaatggc tgagtctctc
     2461 cgaataccag aaggccctat tgatcagggg acagctacag gaagagtccg tgttttagaa
     2521 gaacagcttg ttaaggccaa agaacagatt gaaaattaca agaaacaagc taggaatgat
     2581 ctgggaaagg atcatgaaat cttaaggagg aggattgaaa atggagctaa agagctctgg
     2641 ttttttctac aaagtgaatt gaagaaatta aagaaattag aaggaaacga actccaaaga
     2701 catgcagatg aaattctttt ggatttagga catcatgaaa ggtctatcat gacagatcta
     2761 tactacctca gtcaaacaga tggagcaggt gagtggcggg aaaaagaagc caaagatctg
     2821 acagagctgg tccagcggag aataacatat ctgcagaatc ccaaggactg cagcaaagcc
     2881 agaaagctgg tatgtaatat caacaaaggc tgtggctatg gatgtcaact ccatcatgtg
     2941 gtttactgct tcatgattgc ttatggcacc cagcgaacac tcatcttgga atctcagaat
     3001 tggcgctatg ctactggagg atgggagact gtgtttagac ctgtaagtga gacatgcaca
     3061 gacaggtctg gcctctccac tggacactgg tcaggtgaag tgaaggacaa aaatgttcaa
     3121 gtggtcgagc tccccattgt agacagcctc catcctcgtc ctccttactt acccttggct
     3181 gtaccagaag accttgcaga tcgactcctg agagtccatg gtgatcctgc agtgtggtgg
     3241 gtatcccagt ttgtcaaata cttgatccgt ccacaacctt ggctggaaag ggaaatagaa
     3301 gaaaccacca agaagcttgg cttcaaacat ccagttattg gagtccatgt cagacgcact
     3361 gacaaagtgg gaacagaagc agccttccat cccattgagg aatacatggt acacgttgaa
     3421 gaacattttc agcttctcga acgcagaatg aaagtggata aaaaaagagt gtatctggcc
     3481 actgatgacc cttctttgtt aaaggaggca aagacaaagt actccaatta tgaatttatt
     3541 agtgataact ctatttcttg gtcagctgga ctacacaacc gatacacaga aaattcactt
     3601 cggggcgtga tcctggatat acactttctc tcccaggctg acttccttgt gtgtactttt
     3661 tcatcccagg tctgtagggt tgcttatgaa atcatgcaaa cactgcatcc tgatgcctct
     3721 gcaaacttcc attctttaga tgacatctac tattttggag gccaaaatgc ccacaaccag
     3781 attgcagttt atcctcacca acctcgaact aaagaggaaa tccccatgga acctggagat
     3841 atcattggtg tggctggaaa ccattggaat ggttactcta aaggtgtcaa cagaaaacta
     3901 ggaaaaacag gcctgtaccc ttcctacaaa gtccgagaga agatagaaac agtcaaatac
     3961 cctacatatc ctgaagctga aaaatagtac ccagctttct tgtacaaagt ggtacgcgtg
     4021 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     4081 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     4141 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     4201 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     4261 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     4321 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     4381 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     4441 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     4501 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     4561 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4621 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4681 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4741 tcgtgccagc ggatcgacag tactaagctt ggtgcgtttt tatgcttgta gtattgtata
     4801 atgtttttaa gatccaagct tctgacctct tctcttcctc ccacagggcc tcgagacaat
     4861 tgcatcgatg gtaccagtga aagtctgcaa tgaattcgag ttggcgcgcc tgtcattcta
     4921 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa
     4981 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     5041 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     5101 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     5161 agggggctgt ccccgtgagc tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct
     5221 aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt
     5281 cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg
     5341 ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc
     5401 cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc tgtccccgtg
     5461 agctcccagg cgcgccaact cggtaccgta tcgatgtcga cgttaacgct agtgatatca
     5521 actttgtata gaaaagttgg ctccgaattc gcccttatta attggctccg gtgcccgtca
     5581 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg tggggagggg tcggcaattg
     5641 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     5701 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     5761 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     5821 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     5881 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     5941 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     6001 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     6061 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     6121 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     6181 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     6241 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     6301 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     6361 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     6421 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     6481 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     6541 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     6601 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     6661 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     6721 tcttccattt caggtggatc ccaagggcga attcgaccca agtttgtaca aaaaagcagg
     6781 cttaggaatt catgggccct aaaaagaagc gtaaagtcgc ccccccgacc gatgtcagcc
     6841 tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag
     6901 acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc
     6961 acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta
     7021 ccgatgccct tggaattgac gagtacggtg ggggcgcgcc aactcaagac gaattcaccc
     7081 aactctccca atcaatcgcc gagttccaca cgtaccaact cggtaacggc cgttgctcat
     7141 ctctcctagc tcagcgaatc cacgcgccgc cggaaacagt atggtccgtg gtgagacgtt
     7201 tcgataggcc acagatttac aaacacttca tcaaaagctg taacgtgagt gaagatttcg
     7261 agatgcgagt gggatgcacg cgcgacgtga acgtgataag tggattaccg gcgaatacgt
     7321 ctcgagagag attagatctg ttggacgatg atcggagagt gactgggttt agtataaccg
     7381 gtggtgaaca taggctgagg aattataaat cggttacgac ggttcataga tttgagaaag
     7441 aagaagaaga agaaaggatc tggaccgttg ttttggaatc ttatgttgtt gatgtaccgg
     7501 aaggtaattc ggaggaagat acgagattgt ttgctgatac ggttattaga ttgaatcttc
     7561 agaaacttgc ttcgatcact gaagctatga actacccata cgatgttcca gattacgctt
     7621 gaggatccta cccagctttc ttgtacaaag tggtacgcgt gaattcactc ctcaggtgca
     7681 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     7741 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     7801 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     7861 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     7921 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     7981 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     8041 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     8101 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     8161 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     8221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatcgaca
     8401 gtactaagct tggtctaata cccaatctct cgtcttatcc agatgtttta tacgccaagc
     8461 ttctgacctc ttctcttcct cccacagggc ctcgagacaa ttgcatcgat ggtaccagtg
     8521 aaagtctgca atgaattcga gttggcgcgc ctgtcattct aaatctctct ttcagcctaa
     8581 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     8641 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     8701 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     8761 tagcggggga gggacgtaat tacatccctg ggggctttgg gagggggctg tccccgtgag
     8821 ctcccaggcg cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc
     8881 cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt
     8941 gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag
     9001 cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt
     9061 aattacatcc ctgggggctt tggggggggg ctgtccccgt gagctcccag gcgcgccaac
     9121 tcggtaccgt atcgatgtcg acgttaacgc tagtgatatc aactttgtat agaaaagttg
     9181 gctccgaatt cgcccttatt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg
     9241 cccacagtcc ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     9301 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     9361 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     9421 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     9481 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     9541 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     9601 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     9661 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     9721 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     9781 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     9841 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     9901 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     9961 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
    10021 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
    10081 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
    10141 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
    10201 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
    10261 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
    10321 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtggat
    10381 cccaagggcg aattcgaccc aagtttgtac aaaaaagcag gctggatccc ggtaccctcg
    10441 acacctgctg acaggtgcca ccatggcccg gacccccagc agaagcagca tcggatctct
    10501 gagaagcccc cacacccaca aggccatcct gaccagcacc atcgagatcc tgaaagagtg
    10561 cggctacagc ggcctgagca tcgagtctgt ggctagaagg gctggcgcct ccaagcccac
    10621 catctaccgg tggtggacaa acaaggccgc cctgatcgcc gaggtgtacg agaacgagag
    10681 cgagcaggtc cgaaagttcc ccgacctggg cagcttcaag gccgatctgg acttcctgct
    10741 gcggaacctg tggaaagtgt ggcgggaaac catctgcggc gaggccttca gatgcgtgat
    10801 cgctgaggcc cagctggacc ctgccacact gacccagctg aaggaccagt tcatggaacg
    10861 gcggagagag atgcccaaga aactggtgga aaacgccatc agcaacggcg agctgcccaa
    10921 ggacaccaac cgggaactgc tgctggatat gatcttcggc ttctgctggt acagactgct
    10981 gaccgagcag ctgaccgtgg aacaggatat cgaggaattc acctttctgc tgatcaacgg
    11041 cgtgtgcccc ggcacccaga gagcgttagc acttaaatta gctggtttgg acataggcgg
    11101 cgccagcacg cgtgtgcctt tgtatggttt tacttcgatt tgtggaagaa gacctgagat
    11161 ggaagctgct gtttcgacta taccaagatt ccttcaatct tcctctggtt cgatgttaga
    11221 tggtcggttt gatcctcaat ccgccgctca tttcttcggt gtttacgacg gccatggcgg
    11281 ttctcaggta gcgaactatt gtagagagag gatgcatttg gctttggcgg aggagatagc
    11341 taaggagaaa ccgatgctct gcgatggtga tacgtggctg gagaagtgga agaaagctct
    11401 tttcaactcg ttcctgagag ttgactcgga gattgagtca gttgcgccgg agacggttgg
    11461 gtcaacgtcg gtggttgccg ttgttttccc gtctcacatc ttcgtcgcta actgcggtga
    11521 ctctagagcc gttctttgcc gcggcaaaac tgcacttcca ttatccgttg accataaacc
    11581 ggatagagaa gatgaagctg cgaggattga agccgcagga gggaaagtga ttcagtggaa
    11641 tggagctcgt gttttcggtg ttctcgccat gtcgagatcc attggcgata gatacttgaa
    11701 accatccatc attcctgatc cggaagtgac ggctgtgaag agagtaaaag aagatgattg
    11761 tctgattttg gcgagtgacg gggtttggga tgtaatgacg gatgaagaag cgtgtgagat
    11821 ggcaaggaag cggattctct tgtggcacaa gaaaaacgcg gtggctgggg atgcatcgtt
    11881 gctcgcggat gagcggagaa aggaagggaa agatcctgcg gcgatgtccg cggctgagta
    11941 tttgtcaaag ctggcgatac agagaggaag caaagacaac ataagtgtgg tggtggttga
    12001 tttgaaggat tacaaggacg atgacgataa gtgaagcttc agtgcaggtg aattctacca
    12061 cccagctttc ttgtacaaag tggtacgcgt gaattcactc ctcaggtgca ggctgcctat
    12121 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
    12181 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
    12241 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
    12301 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
    12361 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
    12421 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
    12481 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
    12541 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
    12601 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
    12661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    12721 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    12781 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatcgaca gtactaagct
    12841 tggtgaattc ccttatgtga gtgtaaaagg caggcgagtt tgtcccaagc ttctgacctc
    12901 ttctcttcct cccacagggc ctcgagacaa ttgcatcgat ggtaccagtg aaagtctgca
    12961 atgaattcga gttggcgcgc ctgtcattct aaatctctct ttcagcctaa agctttttcc
    13021 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
    13081 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
    13141 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
    13201 gggacgtaat tacatccctg ggggctttgg gagggggctg tccccgtgag ctcccaggcg
    13261 cgcctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
    13321 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
    13381 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
    13441 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
    13501 ctgggggctt tggggggggg ctgtccccgt gagctcccag gcgcgccaac tcggtaccgt
    13561 atcgatgtcg acgttaacgc tagtgatatc aactttgtat agaaaagttg gctccgaatt
    13621 cgcccttatt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
    13681 ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
    13741 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
    13801 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
    13861 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
    13921 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
    13981 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
    14041 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
    14101 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
    14161 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
    14221 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
    14281 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
    14341 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
    14401 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
    14461 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
    14521 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
    14581 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
    14641 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
    14701 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
    14761 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtggat cccaagggcg
    14821 aattcgaccc aagtttgtac aaaaaagcag gctgatcgcc accatggtgt ctaagggcga
    14881 agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
    14941 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
    15001 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
    15061 catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca
    15121 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
    15181 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
    15241 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
    15301 ctccaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg acatggccct
    15361 gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa
    15421 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat
    15481 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
    15541 cgacctccct agcaaactgg ggcacaaact taattgatac ccagctttct tgtacaaagt
    15601 ggtacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
    15661 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
    15721 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
    15781 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
    15841 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
    15901 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
    15961 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
    16021 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
    16081 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
    16141 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    16201 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    16261 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    16321 gggaaacctg tcgtgccagc ggatcgacag tactaagctt ggttgcttgc aaaagcagta
    16381 attggaaagc actctcaaag aatcccaatt gcatcgatgg taccagtgaa agtctgcaat
    16441 gaattcgagt tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
    16501 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
    16561 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
    16621 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
    16681 gacgtaatta catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg
    16741 cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
    16801 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
    16861 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
    16921 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
    16981 gggggctttg ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtaccgtat
    17041 cgatgtcgac ggtgcgtttt tatgcttgta gtattgtata atgtttttaa gatcctaggg
    17101 ataacagggt aatcggccgc tactagggac aggattggtg acagaaaagc cccatcctta
    17161 ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg ttaggcagat
    17221 tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc cagaacctct
    17281 aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg cagggggtgg
    17341 gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct
    17401 cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc
    17461 agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg
    17521 gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg
    17581 tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg
    17641 tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt
    17701 caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag
    17761 tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc
    17821 cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc
    17881 ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct tccctacagg
    17941 ggttcctggc tctgctctcc accgcgggca gctctggccc gtgtctcaaa atctctgatg
    18001 ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg cttacataaa
    18061 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
    18121 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
    18181 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
    18241 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
    18301 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
    18361 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
    18421 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
    18481 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
    18541 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
    18601 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
    18661 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
    18721 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
    18781 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
    18841 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
    18901 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
    18961 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacg gtatcactag
    19021 tattacagag gtaagttata acagtcgcct aacccaattg aggggataac gcaggaaaga
    19081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    19141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    19201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    19261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    19321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    19381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    19441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    19501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    19561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    19621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    19681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    19741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    19801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    19861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    19921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    19981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    20041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    20101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    20161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    20221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    20281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    20341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    20401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    20461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    20521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    20581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    20641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    20701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    20761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    20821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    20881 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    20941 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    21001 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    21061 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    21121 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    21181 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    21241 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    21301 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
    21361 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    21421 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    21481 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
    21541 gggtaccgta tcg
//
