LOCUS       sequence-239321-       10557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI
  TITLE     HES1 inhibits cycling of hematopoietic progenitor cells via DNA 
            binding.
  JOURNAL   Stem Cells. 2006 Apr . 24(4):876-88.
  PUBMED    16513761
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2006 Apr . 24(4):876-88."
COMMENT     Sequence Label: EF.hICN1.CMV.GFP
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        180..1358
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          411..1349
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1306..1326
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1375..1393
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        3942..4245
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4246..4449
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4400..4420
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4531..4540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4537..4539,4540..4542,4543..5256)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4537..4539,4540..4542,4543..5253)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4582..4603)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4843..4862)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5190..5211
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5364..5597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5669..5790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5706..5725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5760..5779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5809..5944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5965..5983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5993..6010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5993..6009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6002..6024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6151..6606
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6238..6257)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6448..6469
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6632..6736
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6737..6805,6806..7597)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6955..6974)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7768..8356
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8257..8276
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8510..8527
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8644..8665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8680..8685,8686..8703,8704..8710)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8723..8745
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8742..8758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8742..8758
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8777..8797
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8779..8797
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8825..9051
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9081..9261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9308..9433
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9926..10159
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10344..10388
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
       61 ttcgattagt gaacggatct cgacggtatc gataagcttt gcaaagatgg ataaagtttt
      121 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
      181 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      241 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      301 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      361 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      421 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      481 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
      541 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
      601 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
      661 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
      721 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
      781 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
      841 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
      901 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
      961 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     1021 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     1081 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     1141 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1201 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1261 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1321 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg gtactaatac
     1381 gactcactat agggagaccc aagctggcta ggtaagcttg atgatccgga ggcatgcggc
     1441 ggcagcatgg ccagctctgg ttccctgagg gcttcaaagt gtctgaggcc agcaagaaga
     1501 agcggcggga gcccctcggc gaggactccg tgggcctcaa gcccctgaag aacgcttcag
     1561 acggtgccct catggacgac aaccagaatg agtgggggga cgaggacctg gagaccaaga
     1621 agttccggtt cgaggagccc gtggttctgc ctgacctgga cgaccagaca gaccaccggc
     1681 agtggactca gcagcacctg gatgccgctg acctgcgcat gtctgccatg gcccccacac
     1741 cgccccaggg tgaggttgac gccgactgca tggacgtcaa tgtccgcggg cctgatggct
     1801 tcaccccgct catgatcgcc tcctgcagcg ggggcggcct ggagacgggc aacagcgagg
     1861 aagaggagga cgcgccggcc gtcatctccg acttcatcta ccagggcgcc agcctgcaca
     1921 accagacaga ccgcacgggc gagaccgcct tgcacctggc cgcccgctac tcacgctctg
     1981 attccgccaa gcgcctgctg gaggccagcg cagatgccaa catccaggac aacatgggcc
     2041 gcaccccgct gcatgcggct gtgtctgccg acgcacaagg tgtcttccag atcctgatcc
     2101 ggaaccgagc cacagacctg gatgcccgca tgcatgatgg cacgacgcca ctgatcctgg
     2161 ctgcccgcct ggccgtggag ggcatgctgg aggacctcat caactcacac gccgacgtca
     2221 acgccgtaga tgacctgggc aagtccgccc tgcactgggc cgccgccgtg aacaatgtgg
     2281 atgccgcagt tgtgctcctg aagaacgggg ctaacaaaga tatgcagaac aacagggagg
     2341 agacacccct gtttctggcc gcccgggagg gcagctacga gaccgccaag gtgctgctgg
     2401 accactttgc caaccgggac atcacggatc atatggaccg cctgccgcgc gacatcgcac
     2461 aggagcgcat gcatcacgac atcgtgaggc tgctggacga gtacaacctg gtgcgcagcc
     2521 cgcagctgca cggagccccg ctggggggca cgcccaccct gtcgcccccg ctctgctcgc
     2581 ccaacggcta cctgggcagc ctcaagcccg gcgtgcaggg caagaaggtc cgcaagccca
     2641 gcagcaaagg cctggcctgt ggaagcaagg aggccaagga cctcaaggca cggaggaaga
     2701 agtcccagga tggcaagggc tgcctgctgg acagctccgg catgctctcg cccgtggact
     2761 ccctggagtc accccatggc tacctgtcag acgtggcctc gccgccactg ctgccctccc
     2821 cgttccagca gtctccgtcc gtgcccctca accacctgcc tgggatgccc gacacccacc
     2881 tgggcatcgg gcacctgaac gtggcggcca agcccgagat ggcggcgctg ggtgggggcg
     2941 gccggctggc ctttgagact ggcccacctc gtctctccca cctgcctgtg gcctctggca
     3001 ccagcaccgt cctgggctcc agcagcggag gggccctgaa tttcactgtg ggcgggtcca
     3061 ccagtttgaa tggtcaatgc gagtggctgt cccggctgca gagcggcatg gtgccgaacc
     3121 aatacaaccc tctgcggggg agtgtggcac caggccccct gagcacacag gccccctccc
     3181 tgcagcatgg catggtaggc ccgctgcaca gtagccttgc tgccagcgcc ctgtcccaga
     3241 tgatgagcta ccagggcctg cccagcaccc ggctggccac ccagcctcac ctggtgcaga
     3301 cccagcaggt gcagccacaa aacttacaga tgcagcagca gaacctgcag ccagcaaaca
     3361 tccagcagca gcaaagcctg cagccgccac caccaccacc acagccgcac cttggcgtga
     3421 gctcagcagc cagcggccac ctgggccgga gcttcctgag tggagagccg agccaggcag
     3481 acgtgcagcc actgggcccc agcagcctgg cggtgcacac tattctgccc caggagagcc
     3541 ccgccctgcc cacgtcgctg ccatcctcgc tggtcccacc cgtgaccgca gcccagttcc
     3601 tgacgccccc ctcgcagcac agctactcct cgcctgtgga caacaccccc agccaccagc
     3661 tacaggtgcc tgagcacccc ttcctcaccc cgtcccctga gtcccctgac cagtggtcca
     3721 gctcgtcccc gcattccaac gtctccgact ggtccgaggg cgtctccagc cctcccacca
     3781 gcatgcagtc ccagatcgcc cgcattccgg aggccttcaa gtaaacggcg cgtcagatcc
     3841 actagtaacg gccgccagtg tgctggaatt atcgaattcc tgcagcccta atagtaatca
     3901 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     3961 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4021 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4081 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4141 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4201 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     4261 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     4321 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     4381 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     4441 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
     4501 tcaagcttcg aattggggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     4561 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4621 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4681 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4741 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4801 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4861 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4921 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4981 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5041 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5101 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5161 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5221 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgtcga cgggcccgcg
     5281 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5341 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5401 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5461 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5521 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5581 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     5641 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     5701 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5761 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5941 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6001 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6061 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6121 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     6181 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6241 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6301 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6361 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6421 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6481 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6541 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6601 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     6661 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6721 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6781 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6841 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6901 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6961 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7021 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7081 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7141 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7201 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7261 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7321 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7381 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7441 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7501 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7561 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7621 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7681 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7741 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7801 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7861 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7921 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7981 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8041 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8101 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8161 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8221 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     8281 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     8341 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     8401 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     8461 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     8521 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8581 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8641 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8701 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8761 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8821 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8881 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8941 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     9001 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cggtttwmkt
     9061 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     9121 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9181 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9241 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     9301 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9361 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9421 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9481 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9541 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9601 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9661 agtagcaacc cnctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9721 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9781 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9841 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9901 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9961 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10021 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10081 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10141 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10201 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    10261 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    10321 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10381 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10441 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10501 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggg
//
