LOCUS       sequence-239525-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Nanog enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1403_pGL4-UBCmin-luc2/Nanog-B'
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    39..87
                     /note="synthetic polyadenylation signal"
     misc_feature    101..192
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     141..160
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      374..383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             380..2032
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2076..2197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2087..2106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2141..2160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3131..3148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3382..3401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4090..4881,4882..4950))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat
       61 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
      121 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
      181 gaacatttct ctggcctaac tggccggtac ctgagctcgc tagcctcgag gatggggcgg
      241 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
      301 gggatttgaa ttcctcgact ctagaggatc tggcctcggc ggccaagctt ggcaatccgg
      361 tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
      421 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
      481 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
      541 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
      601 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
      661 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
      721 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
      781 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
      841 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
      901 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
      961 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
     1021 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
     1081 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
     1141 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
     1201 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
     1261 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
     1321 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     1381 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
     1441 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
     1501 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
     1561 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
     1621 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     1681 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
     1741 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
     1801 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
     1861 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
     1921 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
     1981 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt aataattcta
     2041 gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     2101 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2161 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2221 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2281 atgtggtaaa atcgataagg atctccctag tccccgctcc ttttcagcac taaccataca
     2341 agttcatctt ttactcttgt tttgactgct aaccacccag aggacccact taacattcct
     2401 ttccccaccc acacagctag ttccaaccag gtctaaggca tctagacgcc ctcctcccta
     2461 ttcaaaccgc ttccctggat aaggaatgta gcaagcctgc cttttggcta ccagccacct
     2521 cttcgctcgg atctttcacc agagactctc aaagacacta aagaggcagg acaggaatgg
     2581 gggttgggga gggatccatc gccgtctcct aagcagactc ctttgacccg gagctgtgcg
     2641 ccctgtacca aacctttgta gaacttgggg taaacttaag gctatggtgg ccttgactcc
     2701 gtggacccag aggcaagttt cctcctttag aggactcgca tgcattttgt ttctaatttg
     2761 aaatgagaac cggcttagag cttgaaccag ccagttctct ggactcctcc cagctcttac
     2821 aattcctctc ccggacggtt cctagaagac aaaggcaagc ttaccaaaat tacgtcgccc
     2881 ttgggacaca cctagggttc cctggtggca tctttttttt ttcattataa acaggagtaa
     2941 atttttgtaa gggcagagct ggtagctgag ggagaggaac cctttggcct gtcgaccgat
     3001 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     3061 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     3121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3481 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3721 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4021 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     4081 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     4141 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     4201 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     4261 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     4321 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     4381 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     4441 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     4501 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     4561 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     4621 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     4681 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     4741 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     4801 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     4861 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     4921 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     4981 ttatcagggt tactagtacg tctctcaagg ataagt
//
