LOCUS       sequence-239628-       18147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full-length murine PC1 for Eukaryotic Expression (982).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18147)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 18147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His
FEATURES             Location/Qualifiers
     source          1..18147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    612..630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    633..651
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     661..685
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             944..1015
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             13823..13852
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             13862..13891
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             13907..13924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(13947..13964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13953..14177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      14223..14651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14310..14329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14520..14541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(14660..14680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14665..14994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14845..14980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14907..14926
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        15042..15089
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             15108..15482
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    15612..15733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15649..15668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15703..15722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15782..15798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15782..15798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15795..15817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15806..15822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15830..15836,15837..15854,15855..15860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15875..15896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16013..16030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16184..16772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16264..16283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16943..17734,17735..17803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17566..17585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17804..17908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(17983..18002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
      661 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      721 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac
      781 cgagctcgga tccactagtc cagtgtggtg gggccctgca gatatccagc acagtggcgg
      841 ccgctctaga gcggccgcca ccatgccgcc cggagcacct gctctcctgg cgctggccct
      901 aggcctgggc ctgtggctcg gagcgctggc cggagaccct ggcgactaca aggacgacga
      961 tgacaaggac tacaaggacg acgatgacaa ggactacaag gacgacgatg acaaacgcgg
     1021 ctgcgggcct tgcccgctcc cctgcttttg cggccctgcg cccgacgccg cctgccgcgt
     1081 caattgctcc ggccgctggc tgcagacgct agggccgagt ctgcgcatcc cggctgacgc
     1141 caccgcgcta gacctgtccc acaacctact tcagacgctg gacatcgggc tcctggtgaa
     1201 cctttcagca ctggtggagc tggacttaag caacaatagg atttctacat tagaagaagg
     1261 cgtgtttgct aatttattta atttaagtga aataaacctg agtgggaacc catttgagtg
     1321 caactgtggt ctggcgtggc tgccacgctg ggcaaaggaa catcaggtgc atgtggtaca
     1381 gtctgaggca accacgtgca gggggcccat tccactggcc ggccaacctc tcctcagtat
     1441 ccccttattg gacaatgcct gtggtgagga atatgtcgcc tgcctccctg acaacagctc
     1501 aggtgctgtg gcagcagtac ccttctactt tgcccatgag ggaccactgg agactgaggc
     1561 ctgcagtgcc ttctgcttct cagctggtga gggccttgca gccctctcgg agcagaatca
     1621 atgcctctgt ggggcaggcc aggcctctaa ctcttctgca gcctgctcat cctggtgttc
     1681 cagcatctca ctgtccctca actctgcctg tgggggcccc accttgctcc agcacacctt
     1741 tcccgcctcc ccaggggcta ccctggtggg gccccatgga cccctggcct ctggccagcc
     1801 agcagacttc catattactt cctccctgcc cattagctca acacgctgga actttggaga
     1861 tggctcacct gaggtggaca tggctagccc tgctgccacc cacttctatg tgctacctgg
     1921 gagctatcac atgacagttg tgctggcctt gggggctggc tcagctctgc tggagacaga
     1981 ggtgcaagta gaagcaacac ctactgtcct ggagcttgtg tgcccatcct ttgtgcacag
     2041 taacgagagc cttgaacttg gcatacggca tcgtggaggc tcagcccttg aggtcaccta
     2101 tagcatcctg gctttggata aagagcctgc ccaagtggtc cacccactct gccccttgga
     2161 cacagagatc ttccctggca atgggcactg ctaccgcctg gtggcagaga aggcaccctg
     2221 gctgcaagca caggagcaat gtcggacttg ggctggagct gccttggcta tggtggacag
     2281 tcctgccatc cagcacttcc tagtctccaa ggttaccagg agcctggatg tgtggatcgg
     2341 cttctcatct gtggagggga cagaaggcct ggatccccgg ggtgaggcct tcagcctgga
     2401 aagctgccag aactggctgc ctggggagcc acacccagcc acagcagagc attgtgtgcg
     2461 gttggggcct gctgggcaat gcaacacaga cctgtgttca gcaccacata gttatgtctg
     2521 cgagctgcgg cctggagggc ctgtgtggga tactgagaac tttgtcatgg gaatgtctgg
     2581 tggaggcctg tctggacctc tgcatccatt ggcacagcag gaaactgtcc agggcccgct
     2641 gcgacctgtg gaggtcatgg tgttccctgg cctgagccct agccgggaag cctttctcac
     2701 tgcagcagag ttttctaccc agaagcttga agagcctgcc cagatgcggc tgcaggtgta
     2761 ccggccctca ggaggagcag cggtcccaga aggcagcagt gagcctgaca acaggactga
     2821 gccggccccc aagtgtgtgc ctgaggaact ctggtgtcca ggagccaatg tctgcatacc
     2881 ttttgatgct tcgtgtaact ctcatgtctg catcaatggg tctgtgtcaa ggctgggact
     2941 ctctagggcc agctacacac tatggaagga gttctttttt tctgtgcctg cagggcctcc
     3001 cacacagtac ttggtcaccc tccacagcca ggatgttcct atgctccctg gtgacctcat
     3061 tggcttacag catgatgctg gcccaggtac tctcctacag tgtcccctgg cttccagctg
     3121 tcctggccaa gccctgtacc tttcaaccaa tgcctcagat tggatgacca acctgcctgt
     3181 ccatctggaa gaagcttggg ctggccctgt ctgctcccta cagctgcttt tggtcacaga
     3241 gcagcttaca ccactgctgg gccttgggcc taaccctgga ctacagcatc ctgggcacta
     3301 tgaggtccgg gctacagtgg gcaacagcgt atccaggcaa aacctgtcct gcagcttcag
     3361 tgtggtctct ccaatagctg ggctgagagt cattcacccc atcccccttg atggccacat
     3421 ctatgtgcca accaacggct cagtcttggt gcttcaggta gattctggtg ctaatgccac
     3481 tgccacagct cagtggtttg ggggcaatat tagtgctccc ttcgaggatg cctgccctcc
     3541 tgaggtggat tttctcaagc aagattgcac agaagaggcc aatggcaccc tgttctcagt
     3601 gttgatgttg cccaggctca aggaggggga tcacacagtg gagattgtgg cgcaaaatgg
     3661 agccagccag gccaacctca gcctgagagt gacagctgag gagcccatct gtggcctccg
     3721 tgctgtgcca agccctgagg cccgagtgct acagggcatc ctggtgcggt acagccccat
     3781 ggtagaggct ggctcagacg tagccttccg gtggaccatt gatgataagc agtctctgac
     3841 tttccacaac actgtcttca atgtaatata ccagagtgct gccgtcttca agctctcgct
     3901 gacagcctct aaccatgtga gcaacatcac cgtgaactac aatgtcactg tggagcggat
     3961 gaacaagatg catgggctgt gggtatctgc agtaccgact gtgttacccc ccaatgccac
     4021 actggcactg acaggtggtg tgctggtgga ttcagctgtg gaggtggcct tcctgtggaa
     4081 ctttggggat ggggagcagg tactccgcca gttcaagcct ccatacgacg agtccttcca
     4141 ggttccagac cccacagtgg cgcaggtgct ggtggaacac aataccacgc atatttacac
     4201 taccccaggt gagtacaact tgaccgtcct tgtgtccaat acctatgaga atctgacgca
     4261 gcaggtgaca gtaagtgtgc gcactgttct tcccaacgtg gctattggca tgagcagcaa
     4321 cgtcctggtg gccggccaac ccatcacctt ttccccatat ccgctgccct caactgatgg
     4381 tgtcctatat acgtgggact ttggggatgg ctcacctgtc ctaatacaga gccagccagt
     4441 actcaaccac acctacagca tgacgggcac ttacagaatc accttggagg ttaacaacac
     4501 agtgagcagc gtgacagcac atgcagacat ccgtgtcttc caggagttgc atggcctgac
     4561 tgtgtatttg agcccatctg tggagcaggg agcccccatg gtagtcagtg cttctgtgga
     4621 gtcaggtgac aacattacat ggacatttga catgggagat ggcacagtat tcacaggccc
     4681 tgaggctaca gtacaacatg tatacctgcg ggcccagaac ttcacagtga cagtagaagc
     4741 agccaaccct gctggccact tgtcccagag cctgcatgtg caagtgtttg tcctggaggt
     4801 attacgcata gagccctcta cctgcatccc cacacagccc agtgcccagc ttatggccca
     4861 tgtcactggg gaccctgtcc attacatctt tgattggact tttggagatg gctcctccaa
     4921 tgtgactgtc catgggcatc catcagtgac acacaacttc actaggagtg gcatattccc
     4981 actggcattg gttttgtcaa gccatgtgaa caaggcacat tattttacca gcatctgtgt
     5041 ggaacctgag atacgtaaca tcaccctgca gcctgagagg cagtttgtga agctggggga
     5101 tgaagcccgg ctggttgcct attcttggcc cccattcccg taccgatata cctgggactt
     5161 tggcactgaa gacactaccc acacccaaac tgggggctct gaagtgaaat ttatctaccg
     5221 agaaccaggc tcctacttgg tgatagtcac tgtgtccaac aacatctctt ctaccaatga
     5281 ctcagctttt gtggaggtcc aggagcctgt gttagtcact ggcatcagga tcaatggctc
     5341 ccatgtgtta gagctgcagc agccttacct gttgtcagcc atgggcagtg ggagccctgc
     5401 cacctaccta tgggaactgg gagacggcag ccagagtgag ggccctgagg tcacccatat
     5461 ctacagcagc accggtgatt tcactgtcag ggtgtctggc tggaatgagg tgagccgtag
     5521 tgaggcccaa ctcaacatca ccgtaaagca gcgtgtgcgg ggcctcacca tcaatgccag
     5581 ccgcacagtg gtgcccctga atggcagtgt gagctttagt accttgttgg aggtagggag
     5641 tgatgtgcat tactcctggg tgctctgtga cagatgcaca cccattccag gcggacccac
     5701 tatttcctac accttccgct ctgtgggcac cttcaatatt attgtcacag ctgagaatga
     5761 ggtgggttct gcccaggaca gcatcttcat ctatgttctc cagttcattg aggggctgca
     5821 ggtggcaggg ggtgataacg gctgctgctt ccctaccaac tacacactgc agctgcaagc
     5881 tgcagttagg gatggcacca acatctctta tagctggaca gcgcagcagg agggcagcct
     5941 catcacactc ttcggcagtg gcaagtgctt ttctctcact gcactcaaag ctagcaccta
     6001 ctatgtccat cttagggcca ctaacatgtt gggcagtgcc gcagccaacc gtactataga
     6061 ctttgtggaa cctgtggaga gtctaatcct atctgcatcc cctaatccag ctgctgtcaa
     6121 catgagtctc accctttgtg ctgaattggc tggtggcagt ggtgttgttt acacttggta
     6181 cctggaggaa ggactgagtt ggaagacctc catgccatct accacccaca cctttgctac
     6241 acctggcctg cacttggtca gagtcacagc tgagaaccag ttgggctcag tcaatgccac
     6301 agttgaagtg gccatacagg tgcctgtggg tggcctgagt atcagaacca gtgagccaga
     6361 tagcatcttt gtggcagctg gctccactct acccttctgg ggtcagctgg ctgaaggtac
     6421 caatgtgact tggtgctgga ccttgccagg tgggagtaag gacagccagt acattgctgt
     6481 gcgcttctcc acggctggca gtttctctct gcagctcaat gcttccaatg cagtcagttg
     6541 ggtctcagcc atgtacaacc tcacagtaga ggaacccatt gtgaacctga tgctgtgggc
     6601 cagcagcaag gtggtggcgc ctgggcagcc agtccacttt gaaatcctgc tggcagctgg
     6661 ctctgctctt actttccgac ttcaggttgg tgggtctgtc cctgaagtgc tccctagccc
     6721 ccacttctcc cacagcttct ttcgggttgg agaccatctg gtgaatgtgc aggctgagaa
     6781 ccacgtgagc catgcccagg cacaggttcg catcctagtg ctggaggctg ttgttgggct
     6841 gcaggtaccc aactgctgtg agccaggcat ggccacaggc actgagaaga acttcacagc
     6901 ccgggtgcag cgaggttccc gagttgccta tgcctggtat ttctccttgc aaaaggttca
     6961 gggtgactca ttggttattt tatctggccg tgatgtcacc tacacacctg tggctgcagg
     7021 gttactggag atccatgtgc gtgcctttaa tgaactgggt ggtgtgaacc tcacgctcat
     7081 ggtggaagtc caggatatca tccagtatgt aacacttcag agtggccgct gctttaccaa
     7141 ccgctcagcc aggtttgagg ctgctacaag ccccagtcca cggcgtgtaa cctaccactg
     7201 ggactttggg gatgggactc cggtacagaa aacagaggaa ttctgggctg atcactacta
     7261 cctgcgtcct ggggactacc atgtagaggt gaatgctaca aacctagtga gtttttttgt
     7321 ggcacaggcc acagtgactg tccaggtgct ggcctgtagg gagcctgaag tggaggtagc
     7381 actgcctctg caggtgctga tgcggcgttc ccagcgcaac tacctggagg cccatgttga
     7441 tctacgcaac tgtgtatcct accagactga atatcggtgg gagatatatc gtactgccag
     7501 ctgccaacgg ccaggacgta tggctcaaat ggtactgcct ggtgtggatg tgagccgacc
     7561 ccagctggtg gtgccacggc tggcactacc tgtgggccac tactgctttg tgtttgtggt
     7621 ttcatttggg gacacaccat tggcacggag catccaggcc aatgtgacag tggctgctga
     7681 gcgtctggta cccatcattg aaggtggctc ataccgggta tggtcagaca cgcaggacct
     7741 ggtgctggat gggagcaagt cctatgaccc taatttagag gatggtgatc agacaccgct
     7801 caacttccat tgggcctgcg tggcttcaac acagagtgag acaggtggct gcgtgctaaa
     7861 ctttgggccc cgtgggagca gtgtggtcac catccccctg gaacgcctag aagctggtgt
     7921 agaatatacc ttcaacctca tagtgtggaa agcaggtcgg aaggaagaag ccaccaacca
     7981 gacagtactg atccgaagtg ggcgagtccc cattgtgtcc cttgagtgtg tgtcctgcaa
     8041 ggcacaagca gtgtatgaag tgagccgaag ttcctatgtg tacttggagg gtcactgcca
     8101 caactgcagc aggggctaca agcaagggtg ttgggccgct cgcactttca gcaataagac
     8161 tctggtgctg aacgagacga caacatccac gggtagcacc ggcatgaatc tggtagtgcg
     8221 gccaggtgca ctgcgtgatg gggagggcta catcttcaca ctcactgtgc tgggccactc
     8281 aggggaagaa gagggctgtg cctcaatccg cctctcaccc aaccgccccc cacttggagg
     8341 ctcctgccgc ctattcccat tggattctgt gcgtggcctc accactaagg ttcacttcga
     8401 gtgcacaggt tggcgtgatg cagaagatgg tggagcccca ctggtatatg ttctgttgct
     8461 gaagcgctgt cgtcagagct actgtgagaa tttctgcatc tataagggta gtctctccac
     8521 ctatggggct gtcctgcctc ctggattcca gccccttttt gtggtgagcc tagctgtggt
     8581 tgtacaagac cagctgggtg ctgctgttgt tgcactcaac aggtctctga ccattgtcct
     8641 accagaaccc agtggaaatc ctgcagacct cgtaccctgg ctgcacagtc tgactgccag
     8701 tgtgctgcct gggctcctga agcaggctga ccctcagcat gtcatcgagt actctttagc
     8761 cctcattact gtgctcaatg agtatgagca ggccccagat gtgtcagagc ccaacgttga
     8821 gcagcagctg agagcccaaa tgcgtaagaa catcacggag accctgatct ccctgcgggt
     8881 caacactgtg gatgacatcc agcagattac tgctgcgtta gcccagtgca tggtgtccag
     8941 cagggagctc atgtgccgct cctgcctgaa gaagatgctg cagaagctgg agggcatgat
     9001 gcgcatcttg caagctgaaa ccactgaggg caccttgaca cccaccacca ttgctgacag
     9061 cattctcaat atcacaggtg acctcatcca cctggccagc ttggacatgc agggcccaca
     9121 gcccttggag ctgggggtgg agccgccctc cctgatggtg gcttctaagg cctacaacct
     9181 ctcgtcggcc ctcatgcgta tcctcatgcg ttcccgagtg ctcaatgagg agcctctgac
     9241 tctggctggc gaggagattg tggccctagg caagcgctca gacccactca gtcttctgtg
     9301 ttatggcaaa gccttggggc ccagctgcca cttctccatt cctgaggcct tcagtggggc
     9361 cctgtcgaac ctcagtgatg tggtgcagct catcttcctt gtggattcca accccttccc
     9421 ctttggctac attagcaact acactgtctc caccaaagtg gcatctatgg cattccagac
     9481 acagactggt acccagatcc ctattgagca gttggctgca gagcgtgcca tcacagtgaa
     9541 ggtgccaaac aactcagacc aggctgccca gagctctcac aatcctgtgg gttctaccat
     9601 tgtacagccc cagacctctg tcagcgctgt ggtcactgca gacaacagta accctcaagc
     9661 tggcctgcat ctgaggatca cttacacagt gcttaatgag cgctatctgt ccgcggagcc
     9721 tgagccctac ctggctgtgt acctgcactc agtgtcccag cccaatgagt acaactgctc
     9781 ggccagcagg aggatcagcc tggaggtgct ggagggtgct gaccacaggc tctacacctt
     9841 cttcattgcc ccaggaactg ggaccttgga caggagttac tacctgaacc tgacgagcca
     9901 tttccactgg tctgccctgg aggtgtctgt gggcctctat acatccctgt gccagtactt
     9961 cagtgaggag atgatgatgt ggagaactga gggcattgta cccctggagg agacctcacc
    10021 cagccaggct gtctgcatta cccgccacct cactgccttc ggtgccagcc tttttgtgcc
    10081 tcccagccat gtccagttca tctttcctga accatctgca agcatcaact acattgtcct
    10141 cctgacgtgt gtcatatgcc tggtgaccta tgtggtcatg gctatgatcc tgcgcaagct
    10201 ggaccagctg gatgttagcc gtgtccgtgt catcccgttc tgtgggaagg ggggccgctt
    10261 caaatatgaa atacttgtta agaccggctg gagccgaggt tcaggtacca ctgctcacgt
    10321 cggcatcatg ctgtatggag aggacaaccg tagtggtcac cggcacttag atggggacag
    10381 agcctttcat cgaaacagcc tggacatctt ccaaattgcc accccgcaca gcctgggcag
    10441 cgtgtggaag atccgagtgt ggcatgataa caaagggctc agccctgcct ggttcctgca
    10501 acacattatc gtccgggacc tacagagtgc ccgcagcacc ttctttttgg tcaatgactg
    10561 gctgtctgtg gagactgagg ccaacggggg cctagtggag aaggaggtgc ttgcagcaaa
    10621 tgaagctgcg ctgtggcagt tccagcggct cctcgtggct gagttgcaga gaggcttctt
    10681 tgacaagcac atctggctct ccatatggga ccggccgcct cgtagccgct ttactagagt
    10741 ccagagggtt acctgctgtg ttctcctcct ctgcctcttc ctggctgcca atgctgtgtg
    10801 gtacggagtt gtgagagaca ccacctacag catgggacct gtgtccagtc tgatctctcc
    10861 tggtgttgac acagttgcta ttggccttgt gtccagtgtg gtagtctacc ctgtctattt
    10921 ggctgtcctg ttcctcttcc ggatgtctcg gagtaaggtg tctggggacc aaaaccccac
    10981 acccacaggg cagcaggccc tagatgttga cagctacctg gacccatctg tgctagacag
    11041 ctccctcctc acactctctg gcctcacaga ggccttcgct ggacaagtga agaatgactt
    11101 gtttctggaa gatgctaaaa gcctggtgtg ctggccatcc agtgagggta ctcttagttg
    11161 gccagacctg ctcagtgacc catctgttgt gagcagcaca ctgcagcgat tgacccaggg
    11221 caggcctggc tgcatgttgg gctcagagga agatggtgcc tctctggtta gcccttcctt
    11281 gcctgccaaa tacttgtcgg cctcagatga agacctgatc catcaggtac tggctgatgg
    11341 ggccaacaac ctggttccta cccaggacac cctcctagaa acagacctgc ttactagcct
    11401 gtccagtgtt cctggagaga aaacagaaac tctgatactg cagacagtgg gggaggagag
    11461 gccagccagc atgggcctga gttgggaaca gtccccggtg acaaggcttt ccaggacagg
    11521 actggtggaa ggttttcaga agcgactgct gcctgcctgg tgtgccccac tggcccatgg
    11581 gctcagcctg ctcttggtag ctgtagctgt agcagtttca gggtggattg gtgccagctt
    11641 ccctcccagc gtgagtgtca tgtggcttct ctcaagcagc tccagcttcc tggcctcctt
    11701 tcttggctgg gagcccctta aggtcctgct ggaagccctc tacttctccc ttgtggccaa
    11761 gcggcttcac cctgatgaag atgacactct ggtagagagc ccagctgtaa cccctgtaag
    11821 tgagcgtgtg ccccgtgtgc ggcccccaca tggttttgca ctcttcttgg caaaggagga
    11881 agctcgaaag gtcaagaggc tccatgacat gttgaagaga ctcttggtgt acatgctttt
    11941 cttactggtg acgctgctgg ccaactatgg ggatgcttct tgccatggtc acgcctatcg
    12001 cctgcagagc gccatcaagc aggagctgga tagccaagcc tttctggcca tcacccggtc
    12061 tgatgagttc tggccatgga tgtcacatgt gttcttgccc tatgtccatg ggaaccagtc
    12121 tagccctgag ctgggccctc cacggctacg gcaggtgcga ctgcaggaag cattctgccc
    12181 agacccttct agctcggagc acatgtgttc agcggcagga agcttgagca ccagtgacta
    12241 tggcattggc tggcagagtg tggtgcaaaa tggctctgag acatgggcct actctgcacc
    12301 cgacctgctg ggcgcctggt actggggcta ctgtgcagtg tatgacagtg ggggctacat
    12361 acaggagtta ggactgagtc tggaggagag ccgtgcacga ctgggcttcc tgcagctgca
    12421 caactggctt gacagcagga gccgtgcagt gtttgtagaa ctcacccgct acagccctgc
    12481 tgtggggctc catgctgctg ttacactgcg cctcgagttc cctgtagccg gccatgcact
    12541 ggctgccttc agtgtccgcc catttgcgtt acggcgactc agcactggcc tgtctctacc
    12601 actgctcacc tcggtgtgct tgctgctgtt tgccctatac ttttccatgg ccgaggtgca
    12661 gacctggcgc aaagatgggt gtgcgtgcac agcaaggcct gacacctggg cacggtgcct
    12721 actggtgata ctgacagcag ctacagggct tgtacgcctg gcccagctgg gcattgctga
    12781 tcgccagtgg acccattttg tgcaggacca tccacgccac ttcactagct ttgaccaagt
    12841 ggctcagctg ggctctgtgg cccgtggcct ggcagcttca ctactcttcc tgcttttggt
    12901 caaggctgcc cagcagctgc ggtttgtgcg ccagtggtct gtttttggca agacattgtg
    12961 tcgggccttg cccgagctca tgggagccac cttgggcctg gtgctgcttg gtgtggccta
    13021 tgcacagatg gccatcctgc ttatttcctc tggtgctgac actctctaca acatggcccg
    13081 tgccttcctg gtgctgtgcc ctggggcgag ggtccccact ctgtgccctt cagagtcctg
    13141 gtacctgtcc cctctgctgt gtgtggggct ctgggctctg cgagtgtggg gtgccctgag
    13201 gttaggggct atcctcctgc gttggcggta ccacgccttg cgtggcgagc tctaccgccc
    13261 agcatgggaa ccccaggact atgagatggt ggagcttttc ctgcgtaggc ttcggctctg
    13321 gatgggcttc agcaaggtca aggagttccg ccacaaagtc cgctttgaag gaatggatcc
    13381 actgccttcc cgctcatcca ggggctccaa gtcatcccca gttgtgctcc cacctagctc
    13441 aggctcagaa gcttcacacc catccacctc gtccagccaa ccagacgggc caagcgccag
    13501 cttaagccgc tcgacgctga agctggaacc agagccctct cgcctccatg ctgtgtttga
    13561 aagtctgctt gtccagtttg accgactcaa ccaggccaca gaggacgtct accagctgga
    13621 gcaacaactc cagagccttc gaggccatgg gcacaatgga cctccttcct ctccctcccc
    13681 tggctgcttc ccaggctctc agccagcttt gcccagccgc ctttctcggg ccagtcaggg
    13741 gctggatcag actgtaggcc ccaacagggt gtccctgtgg cctaataaca aggtccaccc
    13801 cagcagcact gcggccgcac gtgaacaaaa actcatctca gaagaggatc tggggccctt
    13861 cgaacaaaaa ctcatctcag aagaggatct gaatatgcat accggtcatc atcaccatca
    13921 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
    13981 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    14041 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
    14101 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
    14161 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
    14221 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    14281 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    14341 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    14401 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    14461 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    14521 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    14581 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    14641 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    14701 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    14761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    14821 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    14881 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    14941 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    15001 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
    15061 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
    15121 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
    15181 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
    15241 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
    15301 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
    15361 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
    15421 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
    15481 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    15541 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    15601 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    15661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    15721 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
    15781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    15841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    15901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    15961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    16021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    16081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    16141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    16201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    16261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    16321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    16381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    16441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    16501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    16561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    16621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    16681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    16741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    16801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    16861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    16921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    16981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    17041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    17101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    17161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    17221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    17281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    17341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    17401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    17461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    17521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    17581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    17641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    17701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    17761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    17821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    17881 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    17941 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    18001 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    18061 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    18121 tagggttagg cgttttgcgc tgcttcg
//
