LOCUS       sequence-239708-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate; C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-gp160-754*
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(669..687)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        755..859
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(860..928,929..1720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1078..1097)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1891..2479
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2380..2399
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2910..3055
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3098..4135
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4133..4152)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4177..4225
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4879..5258
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5259..5462
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5413..5433
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5459..5483
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7765..7899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7789..7808)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7843..7862
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8042..8064)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acttggacgt ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc
       61 gcccggggct cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg
      121 gttatcaccc cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc
      181 tcccttctct cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca
      241 cactaaaaga gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca
      301 gactcctgcc ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca
      361 gatccaggtc tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc
      421 gcagcccgga aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg
      481 cctcaaattc ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct
      541 cctcttcctc ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg
      601 gtgcggcctg tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag
      661 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      721 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      781 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      841 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      901 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      961 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1021 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1081 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     1141 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1201 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1261 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1321 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1381 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1441 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1501 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1561 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1621 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1681 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1741 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1801 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1861 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1921 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1981 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2041 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2101 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2161 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2221 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2281 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2341 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2401 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2461 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2521 ctggccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa
     2581 cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc
     2641 tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg
     2701 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     2761 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     2821 tgccgccctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg tccccggaag
     2881 aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc gtcttgtcat
     2941 tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata
     3001 ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagcgacccg cttaacagcg
     3061 tcaacagcgt gccgcagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc
     3121 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     3181 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     3241 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     3301 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     3361 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     3421 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     3481 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     3541 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     3601 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     3661 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     3721 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     3781 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     3841 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     3901 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     3961 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     4021 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     4081 tggaaaccga cgccccagca ctcgtccgga tcgggagatg ggggaggcta actgaaacac
     4141 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     4201 cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     4261 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     4321 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     4381 cctgccatag ccactggccc cgtgggttag ggacggggtc ccccatgggg aatggtttat
     4441 ggttcgtggg ggttattatt ttgggcgttg cgtggggtca ggtccacgac tggactgagc
     4501 agacagaccc atggtttttg gatggcctgg gcatggaccg catgtactgg cgcgacacga
     4561 acaccgggcg tctgtggctg ccaaacaccc ccgaccccca aaaaccaccg cgcggatttc
     4621 tggcgtgcca agctagtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     4681 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     4741 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     4801 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     4861 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     4921 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     4981 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     5041 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     5101 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
     5161 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
     5221 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5281 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5341 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
     5401 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5461 ctcgtttagt gaaccgtcag atctctagaa gctgggtacc agctgctagc gccaccatgc
     5521 cgatggggtc tctccagccg ctggccaccc tctacctgtt gggtatgttg gtggcgtccg
     5581 tgctggcgac agagaatctg tgggttacag tctactacgg agttccagtc tggagggacg
     5641 cagagacaac acttttttgt gccagcgacg ctaaagcgta tgacactgaa atgcataatg
     5701 tctgggcaac tcatgcctgt gttcccacag acccgaatcc acaagaaata gacttggaga
     5761 atgtaacgga gaaatttaac atgtggaaaa ataacatggt tgaacaaatg catacggata
     5821 taatcagcct ttgggaccaa tccctgaagc cgtgcgttaa gttgacaccc ttgtgtgtta
     5881 ctctgaactg cacagccatt accaatgata cgagggggaa tgagactggt atcaatcgga
     5941 ccgtcgagac gaccgaaatg accaactgtt cattcaatat gaccactgaa cttagggacc
     6001 gaaaaaagaa ggtcaatgcg cttttttata agcttgacat cgttcagata ggagaaaatt
     6061 catctagcca gtaccgcctc attaattgta ataccagtgt tattacacaa gcctgcccaa
     6121 aagttacctt cgagccaatt ccaattcatt attgcgcgcc tgctggattt gccatcctta
     6181 agtgcaagga caaagagttc aacgggacag gaacttgtag gaacgtgtcc agtgtgcaat
     6241 gcactcacgg tataaaaccc gtcgtgagca cgcaactttt gctcaacggg tccctggcgg
     6301 aaggacaggt gaagatccgg tcagagaaca tctcagacaa tgcaaaaacc atcatcgtcc
     6361 agttgaatga gtctgttcca atcaattgta ctagaccaaa caacaataca cgaaggggga
     6421 tccacctggg tccgggccaa acattctttg ctaccgacat aattggtgac attcgccaag
     6481 ctcactgtaa tgttagcgaa tcaaaatgga acaaggcgct ccaggaagtt gtgaaacagc
     6541 tgaggcagca ctggaacaaa acgataatct tcaaatcctc atccggtggg gatctcgaaa
     6601 tcacgactca ctccttcaac tgtggaggtg agttcttcta ctgtaacacc tcaggcctgt
     6661 tcaatagcac atggaacata gccggcaaca gaacgaatga tacgaagtca aatgaaacaa
     6721 tcaccctccc gtgtcgaata aaacagatcg taaatgtttg gcaaagggta ggacaagcga
     6781 tctacgcgcc cccaattgca ggagtcattc ggtgcaattc aaacatcaca ggcctcctct
     6841 tggtaagaga tgggggagct actaataata cagacgaaac gttccgccct ggaggcggga
     6901 acatgcgcga taattggaga agtgagttgt ataaatataa ggtggtcaag atagagcctc
     6961 tgggcgttgc accgaccagg gcgcgcaggc gcgtggtgga aagggagaag agggctgtag
     7021 gactcggagc cgtatttatt ggatttcttg gggcagcggg ttctaccatg ggtgcagctt
     7081 ccgtgacctt gaccgtacaa gccaggcaac ttctttctgg aatcgttcaa caacaaagca
     7141 atcttctccg agcaatcgag gcccagcagc atctcttgaa acttacagtc tggggcataa
     7201 agcagctcca ggcgcgcgtg ctcgcagttg agagatattt gaaagaccaa cagctgcttg
     7261 gtatttgggg ctgttctgga aaattgatat gcccgactac cgtaccatgg aacagtagct
     7321 ggtcaaacaa atcacaaaac gaaatctggg ataatatgac ctggcttcaa tgggacaagg
     7381 agatttcaaa ttacacacag cttatatatt cattgattga ggaaagccaa aatcagcagg
     7441 agaagaacga gcaggagctg ttggcgctgg acaagtgggc atctttgtgg aattggttcg
     7501 acatttccaa gtggctttgg tatatcagaa tatttataat gatcgtgggc gggttgatag
     7561 gacttagaat tgtattcaca gttctcagca tcattaatag ggttcgccag ggttactccc
     7621 cgctctcttt tcagacacat ccgccatctc caacaggtcc tgataggcct gggcggattg
     7681 aagaagaggg tggtgagcaa ggacgggata gaagcaccag gctggtatct ggattcttgt
     7741 gataactcga ggccggcaag gccggatcca gacatgataa gatacattga tgagtttgga
     7801 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     7861 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     7921 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     7981 aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa
     8041 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8101 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     8161 ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta cgacatctct
     8221 gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca acttgccaac
     8281 tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt ctctgactgt
     8341 agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg
     8401 agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta actcttggct
     8461 gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga agactacggg
     8521 aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg accctcaaga
     8581 gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt agcatatgct
     8641 tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata tgttacccaa
     8701 cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac agcgatatct
     8761 cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac gagggtagtg
     8821 aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg aactctcctg
     8881 aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg ctgagttgtg
     8941 aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa
     9001 aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa ccctcacaaa
     9061 ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt taacaataga
     9121 aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg aatttatggc
     9181 tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat gtgtcccagg
     9241 cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg ggaaagagag
     9301 tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa attaaacggg
     9361 gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt ttgaaattgt
     9421 ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact gtaaaataag
     9481 ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc acttgcccac
     9541 aaaacc
//
