LOCUS       sequence-240104-        1419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs4237150 (GLIS3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs4237150-guide3
FEATURES             Location/Qualifiers
     source          1..1419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..41
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        138..156
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        208..226
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        313..524
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             541..588
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tttaaattat nttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
       61 ttcttggctt tatatatctt gtggaaagga cgaaacaccg aagggccttt agctgtaaat
      121 gttttagagc taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat
      181 aaggctagtc cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt
      241 ggcaccgagt cggtgctttt tttggatcct aggtcttgaa aggagtggga attggctccg
      301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      361 tcggcaattg atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggcgtac ggccaccatg
      541 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagga caagaagtac
      601 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
      661 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
      721 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
      781 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
      841 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
      901 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
      961 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     1021 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     1081 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     1141 atccagctgn tgcagaacta caaccagctt ttccaagaaa aaccccatca acgcccgggg
     1201 gcgtggaacc ccaaggccat ccngtcctgc caaantgaac caaaaaccaa ncggcttgga
     1261 aannntgatt cccccacnct tccccggcga aaagaaagaa taggnccngt tnccgcaacc
     1321 ccgaatgccc cctgaaccng gggccctgaa cccccaaatt ttaaagaaaa cattntccaa
     1381 cctggcccaa aaaattccaa aattgcccct taaacaggc
//
