LOCUS       sequence-240118-        1661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs7163757 (C2CD4A/B GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 C2CD4A/B-enh2-guide2
FEATURES             Location/Qualifiers
     source          1..1661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        139..157
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        209..227
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        314..525
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             542..589
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ttttaaaatt atnttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
       61 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacaag tctgtgtcag
      121 tgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa
      181 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag
      241 tggcaccgag tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc
      301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      481 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggcgta cggccaccat
      541 gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg acaagaagta
      601 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
      661 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
      721 gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa
      781 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
      841 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
      901 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaacg tcgtggacga
      961 ggtggcctac cacagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     1021 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     1081 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     1141 atccagctgg tgcagaccta caaccagctg ttcaaggaaa acccnttcaa cnccgcggnn
     1201 tggaagccaa ggccatcctg tctgccaaac tggacaaaaa cagacggctg gaaaatctga
     1261 tctcccacct gcccggncaa aaaaaaaatg gcctgttccg gaaccagntt gcccgaaacc
     1321 ggggcctgac cccaatttnn naaaaanttt tcaactgggc cagaanatcn caaatggcct
     1381 tgaaaaaggg accccccaaa agaaaactgg gaaacctggn gtcccaaatt ngaaaataac
     1441 ccaacatgtt nttngccnca aaacttgccc aaaccctcct ttggaaaccc ttcaaagtaa
     1501 agctaattac aaancccccc ctagcccctt ttttaaataa aaagcccncc cctttaacct
     1561 tcttganatt tccggggggc ctttctcaaa aaaaaaaaat ttttncnaaa ngaaattccc
     1621 gtattttggg ggccccacaa attaaatttt cccccctgta t
//
