LOCUS       sequence-240158-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from
            S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 CRY2-TSS-guide2
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        67..85
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        137..155
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        242..453
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             470..517
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tnnnannnnn tntgnaanga cgaaacaccg gggcggaccc acacatggtg ttttagagct
       61 aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata aggctagtcc
      121 gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg gcaccgagtc
      181 ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
      241 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      301 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      361 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      421 ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga aaaggccggc
      481 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca gcatcggcct
      541 ggccatcggc accaactctg tgggctgggc cgagatcacc gacgagtaca aggtgcccag
      601 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
      661 agnnnngctg ttccgacagn cggcgaaaca nccgaaggcc acccggctga anagaaccgc
      721 ccgaaganaa tacgccagaa cnnannannc gggatctggc tnnntggcag cagatcgtcc
      781 nncaacgaag atggtcgann gtgggacgaa gagctttcnn tccacataac tgggaaggag
      841 tcctttcctg ggtggaaaga tggataagna agc
//
