LOCUS       sequence-240200-        1250 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of human islet enhancer containing 
            T2D-associated variant rs58692659 (ZFAND3 GWAS locus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 rs58692659-guide3
FEATURES             Location/Qualifiers
     source          1..1250
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     11..30
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        127..145
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        197..215
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        302..513
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             530..577
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tttaaaatng gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
       61 atatatcttg tggaaaggac gaaacaccga gttgccaggc agttgagccg ttttagagct
      121 aggccaacat gaggatcacc catgtctgca gggcctagca agttaaaata aggctagtcc
      181 gttatcaact tggccaacat gaggatcacc catgtctgca gggccaagtg gcaccgagtc
      241 ggtgcttttt ttggatccta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
      301 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      361 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      421 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      481 ctttttcgca acgggtttgc cgccagaaca caggcgtacg gccaccatga aaaggccggc
      541 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggac aagaagtaca gcatcggcct
      601 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
      661 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
      721 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
      781 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
      841 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggngggaag
      901 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtgggacga ggtgggccta
      961 ccacgaagaa gtaccccacc atctaccacc tgaagaaaga aactggtgga acagcaaccg
     1021 acaagggccg acctgccggt tgatctatct gggccctggg ccaccatgat caagtttccg
     1081 ggggccactt cctgaatcan gggcgaacct gaaaccccga acaacagcgg acgtgggaca
     1141 aagctgtttc atcccagtgg ggggcaaaac ctaaaaccca ggttnttcca aggaaaaacc
     1201 cccattaaac gtcaggtngc gttggaaccc ccaatgtccn tctcctgttt
//
