LOCUS       sequence-240210-         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 
            from S. pyogenes and VP64 with 2A-BlastR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Miguel-Escalada I, Bonas-Guarch S, Cebola I, Ponsa-Cobas J, 
            Mendieta-Esteban J, Atla G, Javierre BM, Rolando DMY, Farabella I, 
            Morgan CC, Garcia-Hurtado J, Beucher A, Moran I, Pasquali L, 
            Ramos-Rodriguez M, Appel EVR, Linneberg A, Gjesing AP, Witte DR, 
            Pedersen O, Grarup N, Ravassard P, Torrents D, Mercader JM, Piemonti
            L, Berney T, de Koning EJP, Kerr-Conte J, Pattou F, Fedko IO, Groop 
            L, Prokopenko I, Hansen T, Marti-Renom MA, Fraser P, Ferrer J
  TITLE     Human pancreatic islet three-dimensional chromatin architecture 
            provides insights into the genetics of type 2 diabetes.
  JOURNAL   Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0.
  PUBMED    31253982
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 
            10.1038/s41588-019-0457-0."
COMMENT     Sequence Label: lentiSAMv2 ZFAND3-guide4
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        129..147
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        199..217
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 atnntttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
       61 tttatatatc ttgtggaaag gacgaaacac cgcagcgcag cctccggggg agttttagag
      121 ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa taaggctagt
      181 ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag tggcaccgag
      241 tcggtgcttt ttttggatcc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      301 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcnatt
      361 gatccggtgc ctananaang tggcgcggng tanactgnna aantnatgtc nngnantgnc
      421 tccncctttt tc
//
