LOCUS       sequence-240242-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integrating mCherry into lambda attB in E. coli chromosome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Cui L, Vigouroux A, Rousset F, Varet H, Khanna V, Bikard D
  TITLE     A CRISPRi screen in E. coli reveals sequence-specific toxicity of 
            dCas9.
  JOURNAL   Nat Commun. 2018 May 15;9(1):1912. doi: 10.1038/s41467-018-04209-5.
  PUBMED    29765036
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-15"; volume: "9(1):1912. doi"; pages: " 
            10.1038/s41467-018-04209-5"
COMMENT     Sequence Label: pOSIP-KL-mcherry
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      11..97
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      194..280
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      377..463
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tccnnggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
       61 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
      121 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggaatta
      181 attccccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      241 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
      301 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa
      361 ttaattcccc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      421 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
      481 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
      541 gaattgggga tcggcctatg aactgtcgac tcgaggcctt gacaggtacc tcatggatac
      601 ctataatggt tccggaattc ctttaaagag gggaaatcta gatggtttcc aagggcgagg
      661 aggataacat ggctatcatt aaagagttca tgcgcttcaa agttcacatg gagggttctg
      721 ttaacggtca cgagttcgag atcgaaggcg aaggcgaggg ccgtccgtat gaaggcaccc
      781 agaccgccaa actgaaagtg actaaaggcg gcccgctgcc ttttgcgtgg gacatcctga
      841 gcccgcaatt tatgtacggt tctaagctta tgttancacc cagcggatat cccggactat
      901 ctgaagctgt cttttccgga aggtttcaag nngnaacgcg taatgaattt tgaagatggn
      961 ggng
//
