LOCUS       sequence-240244-        5886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Lee SK, Keasling JD
  TITLE     A propionate-inducible expression system for enteric bacteria.
  JOURNAL   Appl Environ Microbiol. 2005 Nov . 71(11):6856-62.
  PUBMED    16269719
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2005 Nov . 71(11):6856-62."
COMMENT     Sequence Label: pPro33
FEATURES             Location/Qualifiers
     source          1..5886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1969..1988)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      2064..2609
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2510..2529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2726..2743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3138..3240
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3241..3900
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3307..3326)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      complement(4456..4911)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4593..4614)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4805..4824
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        complement(5369..5460)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5479..5506)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5598..5684)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5837..5854
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgct
       61 agcttgttat caacttgtta tttgcgttga taaagacaaa gcaaggggtg tgccacaatt
      121 gccttaataa aattaaatgt ttaatttcaa tttgttagag ataaagtcat gaactaatcc
      181 gcaaatatgc gtttcagtta acgtttcagg caatgtttca ccgcgtttca ttgcaacaat
      241 tatgaaacaa gactaaaccc aatattcggt ttcttaactt tgcggtgcgc tatggcacat
      301 ccaccacggc ttaatgacga caaaccggtt atctggacgg tatctgtaac gcgcctgttc
      361 gagctgtttc gcgatatcag cctcgagttt gatcacctgg cgaacattac ccctatccag
      421 cttggctttg aaaaagcggt gacctacatc cacaagaaac tggcaaacga acgctgtgac
      481 gccatcatcg ccgctggatc taacggcgcg tacctgaaaa gccgcctgtc agtgccagtt
      541 attttgatta aaccgagcgg ctacgatgtg ttacaggcac tggcaaaagc cggaaaactc
      601 acctcttcta tcggcgttgt cacttatcag gaaactattc cggcactggt ggcgtttcaa
      661 aaaaccttta atttgcgcct cgatcaacgt agctacatta ccgaagaaga cgcacgcggg
      721 cagattaacg agctaaaagc taacggcacc gaagcggtgg tcggcgcggg gctgattacc
      781 gatctggcag aagaagccgg aatgaccgga atttttatct attccgccgc caccgtgcgc
      841 caggcgttca gcgatgcgct ggatatgacg cgcatgtcgt tacgccataa cactcacgat
      901 gccacccgca acgccctgag aactcgttac gtgctgggcg atatgctcgg tcaatcacca
      961 cagatggaac aagtacggca gactattttg ctgtatgccc gctccagtgc agcggtgttg
     1021 attgaggggg aaacggggac gggcaaagag ctggcggccc aggcgattca tcgggaatat
     1081 tttgcccgcc acgatgcgcg acagggcaaa aagtcgcatc cgtttgttgc agtcaactgc
     1141 ggggcgattg ccgaatcgct gctggaagca gaactgtttg gctatgagga aggggcgttt
     1201 accggctcgc gacgcggcgg tcgcgccggg ctgtttgaaa tcgcccacgg aggtacgctg
     1261 tttctcgatg agattggcga aatgccgctg ccgttgcaga cccggctgct gcgggtgctg
     1321 gaagaaaaag aggtcacccg cgtcggcggg catcagcctg ttccggtgga tgtgcgggtc
     1381 attagcgcca ctcactgcaa tctggaagaa gatatgcggc aagggcagtt tcgccgtgac
     1441 ctgttttatc ggctgagtat tttgcgtctg caattgccac cactgcgcga gcgggtggcg
     1501 gatattctgc cactggcgga aagctttttg aaagtgtctc tggcggcgct ctccaccccg
     1561 ttttctgccg cattacgcca gggattacag gcaagcgaaa ccgtgctggt gcactacgac
     1621 tggccgggca atattcgtga actgcgcaat atgatggagc gactggcgct atttttaagt
     1681 gtggaaccga cgccggattt aacgccgcaa tttttgcagc tgctactgcc ggaactggcg
     1741 cgcgagtcgg cgaaaactcc cgctccacgc ttactgacac cacaacaggc actggagaaa
     1801 tttaatggcg ataaaacagc agcggcgaat tatttaggca tcagccggac gacgttctgg
     1861 cggcggctga aaagctgaat cgatgataag ctgtcaaaca tgagcagatc ctctacgccg
     1921 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     1981 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagccta gaaatatttt
     2041 atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct
     2101 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct
     2161 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag
     2221 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc
     2281 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc
     2341 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
     2401 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     2461 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg ggaaacgcct
     2521 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     2581 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct
     2641 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc
     2701 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct
     2761 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact
     2821 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tactagcgct
     2881 gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac
     2941 agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt
     3001 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
     3061 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     3121 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
     3181 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     3241 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     3301 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     3361 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     3421 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     3481 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     3541 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     3601 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     3661 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     3721 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     3781 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     3841 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     3901 tttttttaag gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat
     3961 aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc
     4021 agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa
     4081 gcagtgtgac cgtgtgcttc tcaaatgcct gactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4441 cacctagatc cttttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     4501 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4561 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4621 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4681 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4741 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4801 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4861 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg taaatcaatc
     4921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5221 gtaagtagtt cgccagttaa tagtttgctg cacccaactg atcttcagca tcttttactt
     5281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5461 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
     5521 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
     5581 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     5641 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     5701 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
     5761 cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct gttttatcag
     5821 accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc atccgccaaa
     5881 acagcc
//
