LOCUS       sequence-240373-       11287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711-QES.c11
FEATURES             Location/Qualifiers
     source          1..11287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2251..2396
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2439..3476
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3474..3493)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3518..3566
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4220..4599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4600..4803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4754..4774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4800..4824
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7427..7561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7451..7470)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7505..7524
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7704..7726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     1921 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     1981 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt
     2041 gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt
     2101 cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
     2161 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     2221 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     2281 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat
     2341 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     2401 gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct gaactcaccg
     2461 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     2521 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     2581 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     2641 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga
     2701 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     2761 tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     2821 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     2881 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     2941 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     3001 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     3061 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     3121 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     3181 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     3241 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     3301 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     3361 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     3421 gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct aactgaaaca
     3481 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     3541 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     3601 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     3661 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     3721 ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg gaatggttta
     3781 tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga ctggactgag
     3841 cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg gcgcgacacg
     3901 aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc gcgcggattt
     3961 ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     4021 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     4081 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     4141 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     4201 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     4261 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     4321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4381 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4441 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     4501 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     4561 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4621 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4681 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     4741 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4801 gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag cgccaccatg
     4861 cctatgggga gcctccaacc cctggctaca ctctatttgc ttggtatgct cgtagccagt
     4921 gtcttggcta atctctgggt cacagtatat tatggtgtac ccgtatggaa ggaagctaag
     4981 accaccctct tctgtgcgtc tgacgcgaag ggctacgaca aggaagttca caatgtttgg
     5041 gctactcacg catgtgttcc gactgatccc aacccgcaag agatgcctct ggagaatgtt
     5101 accgagaatt tcaatatgtg ggaaaatgac atggtgaatc aaatgcacga agatgtgatc
     5161 agtctgtggg atgagtcctt gaagccttgc gtcaaactga cccctctgtg cgtaactttg
     5221 aactgcacgg atgttaataa aaatgtttcc tcttccgaca cggataacta caaagagact
     5281 atgaaggagc ggaaaaattg cacgttcaat atgacgacag agcttcgcga caagaaccag
     5341 aagaaatatg cccttttcta taagctcgac ctggtcccac tggatgataa tgacaatgcg
     5401 tcataccggc ttataaattg caatacgagc acgctgactc aggcctgccc aaagatgagt
     5461 tttgacccta taccgatcca ctactgtgcc cctgcagggt acgcaattct caaatgcaag
     5521 aataaaacct tcaatgggat agggccgtgt aacaaggttt caaccgttca atgcacgcat
     5581 ggaataaagc cgaccgtatc tacacaactt ttgcttaacg ggagtcttgc ggaagaggac
     5641 attgttattc gctcagagaa cataaccgat aatgcgaaaa caataatcgt acacctcaat
     5701 gagtccgtgg agatcgtttg catccgccct aataataaca ctcggaaatc tattaggata
     5761 ggtccaggcc aaacctttta cgcgacgggt gatatagttg gggacatccg ccaggcatac
     5821 tgcaatatct cagaggggaa atggaataag actctccagc gggtatctga gaagctggct
     5881 gagcacttcc cgaacagtac aattaacttc aatagctcaa gcgggggtga cctggagatt
     5941 acgacgcatt ctttcaattg tggcggggaa tggttctatt gcaatacgtc tggcctcttc
     6001 aacggaacct atatgaacaa tgatacgaag agcaatgaca caaagtctaa cagttcatca
     6061 atcatcacga tcccatgtcg cattaagcaa attatcaaca tgtggcaaga agtcggacgc
     6121 gcggtatacg caccacccat cgccggtaac attacttgta aatccaacat tacgggcatt
     6181 cttctgacta gggatggtgg gagaggcgaa gaagtgaaaa atgataccga gacatttagg
     6241 ccaggagggg gcaacatgaa agacaattgg aggagtgagc tttataaata caaagtagtt
     6301 gaaatcaagc ccctcggagt ggcaccaacc gccgccaagc gcagagtcgt cgagagagaa
     6361 aagagggcag taggacttgg ggcggtgctc cttggatttc ttggggcagc cgggagtacg
     6421 atgggcgcag cttccatcac tttgacggtt caggcgcgac aactcctctc tggaattgtt
     6481 caacagcagt ctaacttgct ccgggccatt gaggcgcagc agcacatgtt gcagctcacc
     6541 gtgtggggta ttaagcagct ccaagctagg gtacttgcca tcgagcggta tcttaaggat
     6601 caacagctcc tcggaatttg ggggtgttcc ggtaagctta tttgcacaac agcggtcccc
     6661 tggaactcca gttggtctaa taagaatcaa actgagatat gggacaagat gacttggatg
     6721 caatgggata gagagatttc aaattatacg gacactatat accgccttct tgaagatagc
     6781 caaaaccaac aggaaaagaa tgagaaggac cttttggaac ttgacaagtg gcagaacctt
     6841 tggagttggt tcaacataac taactggctc tggtacataa gaatattcat aatgattgta
     6901 gggggcttga taggcttgcg aataatattc gcggttctgt caatcgtaaa ccgcgtacga
     6961 caaggatatt caccgttgtc ttttcagacc ctcgcgccga atcctcgggg attggaccgc
     7021 ctggggcgga ttgaagaaga gggagggaaa gaggacagga acagatccat tcgacttgtc
     7081 cacggttttc ttgcgcttgc gtgggacgat ttgcggtcac tctgtctgtt tagctaccac
     7141 cggctcagag accttatctt gctcattgcg cgcgctgttg aattgctggg tcaacgcggg
     7201 tgggaagcgc ttaaatatct cgcggggata gttcaatatt ggggtcttga attgaaaaaa
     7261 tccgcagtat cactgttcga taccatagcg attgccgtcg cagagagaac tgaccgaata
     7321 atagggttga tacagggcat ctgccgcgca atctgcaata tacccaggcg gattcgacag
     7381 ggcttcgagg cagctctcca gtagtaactc gaggccggca aggccggatc cagacatgat
     7441 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     7501 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7561 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     7621 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     7681 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     7741 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     7801 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     7861 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     7921 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     7981 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     8041 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     8101 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     8161 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     8221 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     8281 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     8341 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     8401 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     8461 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     8521 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     8581 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     8641 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     8701 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     8761 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     8821 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     8881 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     8941 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     9001 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     9061 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     9121 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     9181 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     9241 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     9301 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     9361 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     9421 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     9481 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     9541 ctatcctaat ctrtatcygg gtagcatatg ctatcctaat mgagattagg gtagtatatg
     9601 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     9661 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     9721 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     9781 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     9841 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     9901 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
     9961 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt
    10021 tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc
    10081 tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag
    10141 tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa
    10201 gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta
    10261 gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac
    10321 tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca
    10381 ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg
    10441 tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg
    10501 tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa
    10561 taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca
    10621 aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc
    10681 cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt
    10741 ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca
    10801 atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct
    10861 tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg
    10921 tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca
    10981 ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta
    11041 cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg
    11101 ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg
    11161 gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta
    11221 cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc
    11281 cctcctg
//
