LOCUS       sequence-240413-        9391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; 
            DS-SOSIP.6mut variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.DS-SOSIP.6mut-gp160
FEATURES             Location/Qualifiers
     source          1..9391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(432..450)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        518..622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(623..691,692..1483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPXAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(841..860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1654..2242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2143..2162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2673..2818
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2861..3898
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3896..3915)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3940..3988
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4642..5021
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5022..5225
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5176..5196
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5222..5246
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7876..8010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7900..7919)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7954..7973
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8153..8175)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcacactaaa agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt
       61 gcagactcct gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag
      121 acagatccag gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta
      181 ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc
      241 aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc
      301 cctcctcttc ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc
      361 tgggtgcggc ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg
      421 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      481 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      781 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      841 gctatgtggc gcggtattat cccgtrttga cgccgggcaa gagcaactcg gtcgccgcat
      901 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      961 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1021 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1081 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1141 cgacgagcgt gacaccacga tgcctgyagc aatggcaaca acgttgcgca aactattaac
     1201 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1261 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1321 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1381 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1441 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1801 ycttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2281 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     2341 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     2401 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc
     2461 gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca
     2521 ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
     2581 aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg
     2641 aagaaatata tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt
     2701 cattggcgaa ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc
     2761 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca
     2821 gcgtcaacag cgtgccgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac
     2881 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2941 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     3001 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     3061 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     3121 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     3181 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     3241 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     3301 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     3361 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     3421 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     3481 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     3541 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     3601 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     3661 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3721 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3781 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3841 tagtggaaac cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa
     3901 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     3961 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     4021 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     4081 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     4141 ggccctgcca tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt
     4201 tatggttcgt gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg
     4261 agcagacaga cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca
     4321 cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat
     4381 ttctggcgtg ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     4441 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     4501 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     4561 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     4621 gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
     4681 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4741 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     4801 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     4861 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
     4921 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
     4981 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     5041 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     5101 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     5161 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     5221 gagctcgttt agtgaaccgt cagatctcta gaagctgggt accagctgct agcgccacca
     5281 tgccaatggg ctcccttcag cccttggcta ccctgtacct tcttgggatg ttggtcgcat
     5341 ctgtgcttgc cgcagaaaac ttgtgggtaa ctgtctacta tggagtaccc gtttggaaag
     5401 acgcagaaac aacgcttttt tgcgcatccg acgcgaaggc atacgaaacg gaaaagcaca
     5461 atgtttgggc aacgcatgcc tgcgtcccga cagacccaaa tcctcaggag attcaccttg
     5521 agaatgtaac agaggaattc aacatgtgga agaataacat ggtagaacaa atgcatacgg
     5581 atattatcag tctttgggac cagagcctta aaccttgcgt taaacttacg cccctctgcg
     5641 taactctgca atgtactaac gtaacgaata acattacgga cgacatgcgc ggcgaaatga
     5701 agaattgcag ctttaatatg acaacggagt tgcgagataa aaagcagaag gtgtacagcc
     5761 tcttctggag gcttgacgtt gtgcaaataa acgaaaacca ggggaacagg agcaataact
     5821 ccaataaaga atacagattg atcaactgta acactagcgc atgtacccag gcctgcccga
     5881 aggtgtcatt tgagccgatt cctatacact attgcgcccc ggcagggttt gcgatcttga
     5941 aatgcaagga taagaagttt aatggaactg gtccctgtcc cagtgtgagt acggttcagt
     6001 gtacccacgg cataaagcca gtggtatcca ctcagcttct cctcaatggc agcctcgcag
     6061 aggaagaggt catgatacgg agtgagaata ttactaacaa tgccaagaat atcttggtcc
     6121 aatttaatac ccccgtccag attaattgta cccgacctat gaatatgacc agaaaatcca
     6181 ttcgcatcgg accaggtcag gcgttctatg ccctgggaga tataataggt gacatccgac
     6241 aggcgcattg caatgtaagc aaggccacat ggaatgagac tctgggtaaa gttgtcaagc
     6301 agctcaggaa gcatttcggg aacaatacca tcatccgatt tgcgaacagt agtgggggtg
     6361 atcttgaagt aactacacat tcatttaact gcggcgggga gtttttttat tgtaacacat
     6421 ccgggttgtt caactccact tggatatcta acactagtgt tcagggatca aactccacag
     6481 gatcaaacga tagtataaca cttccttgtc gcatgaaaca gattatcaac atgtggcagc
     6541 gaatcggcca gtgcatgtac gccccgccta tccagggagt aatacggtgc gtaagcaaca
     6601 taacgggcct tatacttacg cgcgatggag gcagcactaa ttctaccact gagacgttcc
     6661 ggcctggcgg tggggacatg cgagacaact ggcggtctga gttgtacaag tacaaagtgg
     6721 ttaaaatcga acccctgggt gtcgccccaa cgagatgtaa acgacgggta gtgggaagga
     6781 ggagaaggcg acgggcggtc ggcattgggg ccgttttctt gggttttctg ggggcggctg
     6841 ggagcactat gggagcagct agtatgaccc tgaccgtaca ggcgaggaac ctcctgtccg
     6901 gtatagtgca acaacagtca aaccttctta gggctcctga ggcgcagcaa catctcctta
     6961 agttgactgt ctggggcata aagcaactcc aagcgcgggt tctcgcggtg gaaagatacc
     7021 ttagggacca acaacttttg gggatttggg gttgttcagg caaactcatc tgttgcacta
     7081 acgttccttg gaactcctca tggtctaacc gaaatctctc cgagatctgg gataatatga
     7141 catggttgca gtgggataag gagattagca attacactca aataatctat gggctcctgg
     7201 aggaaagcca gaatcagcag gagaagaatg aacaggatct tctggccctt gataagtggg
     7261 caagtctttg gaattggttc gacatttcaa attggctttg gtatataaag atatttataa
     7321 tgattgtcgg gggtttgata ggtttgagga ttgtgtttgc tgtgctgtct gttatccaca
     7381 gggttaggca ggggtatagt ccgctctctt ttcaaaccca tacccccaac cccaggggat
     7441 tggaccgacc cgagcggatt gaggaggaag acggtgaaca ggaccggggc agaagtaccc
     7501 gccttgttag cgggtttctg gctctggcgt gggacgactt gcggagcctt tgcctcttct
     7561 gctaccatcg cctcagagat tttatcttga tcgctgccag aatagtagaa ttgttgggcc
     7621 acagctccct caaaggtttg agacttggat gggaaggtct taaatacctg tggaatttgc
     7681 tggcttactg gggtagggag ctgaagattt ctgccattaa cttgttcgac acaatagcta
     7741 ttgcagttgc ggagtggact gacagagtca tagaaatagg ccaaagattg tgtcgggcgt
     7801 ttctccacat tccacggcgg attaggcaag gacttgaacg ggccctcctt tagtaactcg
     7861 aggccggcaa ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     7921 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7981 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     8041 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     8101 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     8161 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     8221 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc
     8281 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     8341 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     8401 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     8461 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     8521 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     8581 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     8641 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     8701 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     8761 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     8821 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     8881 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     8941 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     9001 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     9061 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     9121 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     9181 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     9241 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     9301 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     9361 agacaggtga accatgttgt tacactctat t
//
