LOCUS       sequence-240414-       11825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate 
            (containing SOSIP mutations).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-gp160
FEATURES             Location/Qualifiers
     source          1..11825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(466..484)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        552..656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(657..725,726..1517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(875..894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1688..2276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2177..2196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2707..2852
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2895..3932
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3930..3949)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3974..4022
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4676..5055
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5056..5259
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5210..5230
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5256..5280
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7910..8044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7934..7953)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7988..8007
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8187..8209)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8769..10558
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat
       61 ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc
      121 cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca
      181 tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga
      241 acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt
      301 ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca
      361 gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt
      421 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1801 ctggcttcag cagagcgcag ataccaaata ctgtycttct agtgtagccg tagttaggcc
     1861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2281 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat
     2341 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
     2401 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
     2461 cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     2521 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     2581 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg
     2641 gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt
     2701 tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
     2761 cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg
     2821 aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg
     2881 gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     2941 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     3001 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     3061 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     3121 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     3181 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     3241 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     3301 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     3361 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     3421 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     3481 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     3541 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     3601 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     3661 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     3721 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     3781 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     3841 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     3901 gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga
     3961 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
     4021 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     4081 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     4141 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt
     4201 gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg
     4261 ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat
     4321 ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca
     4381 aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca
     4441 attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag
     4501 gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca attagccata
     4561 ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat
     4621 ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat
     4681 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4741 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4801 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4861 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4921 tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4981 tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc
     5041 atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt
     5101 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     5161 caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc
     5221 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     5281 tctagaagct gggtaccagc tgctagcgcc accatgccaa tgggctccct tcagcccttg
     5341 gctaccctgt accttcttgg gatgttggtc gcatctgtgc ttgccgcaga aaacttgtgg
     5401 gtaactgtct actatggagt acccgtttgg aaagacgcag aaacaacgct tttttgcgca
     5461 tccgacgcga aggcatacga aacggaaaag cacaatgttt gggcaacgca tgcctgcgtc
     5521 ccgacagacc caaatcctca ggagattcac cttgagaatg taacagagga attcaacatg
     5581 tggaagaata acatggtaga acaaatgcat acggatatta tcagtctttg ggaccagagc
     5641 cttaaacctt gcgttaaact tacgcccctc tgcgtaactc tgcaatgtac taacgtaacg
     5701 aataacatta cggacgacat gcgcggcgaa ctgaagaatt gcagctttaa tatgacaacg
     5761 gagttgcgag ataaaaagca gaaggtgtac agcctcttct acaggcttga cgttgtgcaa
     5821 ataaacgaaa accaggggaa caggagcaat aactccaata aagaatacag attgatcaac
     5881 tgtaacacta gcgcaataac ccaggcctgc ccgaaggtgt catttgagcc gattcctata
     5941 cactattgcg ccccggcagg gtttgcgatc ttgaaatgca aggataagaa gtttaatgga
     6001 actggtccct gtcccagtgt gagtacggtt cagtgtaccc acggcataaa gccagtggta
     6061 tccactcagc ttctcctcaa tggcagcctc gcagaggaag aggtcatgat acggagtgag
     6121 aatattacta acaatgccaa gaatatcttg gtccaattta atacccccgt ccagattaat
     6181 tgtacccgac ctaataataa taccagaaaa tccattcgca tcggaccagg tcaggcgttc
     6241 tatgccacgg gagatataat aggtgacatc cgacaggcgc attgcaatgt aagcaaggcc
     6301 acatggaatg agactctggg taaagttgtc aagcagctca ggaagcattt cgggaacaat
     6361 accatcatcc gatttgcgaa cagtagtggg ggtgatcttg aagtaactac acattcattt
     6421 aactgcggcg gggagttttt ttattgtaac acatccgggt tgttcaactc cacttggata
     6481 tctaacacta gtgttcaggg atcaaactcc acaggatcaa acgatagtat aacacttcct
     6541 tgtcgcatca aacagattat caacatgtgg cagcgaatcg gccaggcgat gtacgccccg
     6601 cctatccagg gagtaatacg gtgcgtaagc aacataacgg gccttatact tacgcgcgat
     6661 ggaggcagca ctaattctac cactgagacg ttccggcctg gcggtgggga catgcgagac
     6721 aactggcggt ctgagttgta caagtacaaa gtggttaaaa tcgaacccct gggtgtcgcc
     6781 ccaacgagat gtaaacgacg ggtagtggga aggaggagaa ggcgacgggc ggtcggcatt
     6841 ggggccgttt tcttgggttt tctgggggcg gctgggagca ctatgggagc agctagtatg
     6901 accctgaccg tacaggcgag gaacctcctg tccggtatag tgcaacaaca gtcaaacctt
     6961 cttagggctc ctgaggcgca gcaacatctc cttaagttga ctgtctgggg cataaagcaa
     7021 ctccaagcgc gggttctcgc ggtggaaaga taccttaggg accaacaact tttggggatt
     7081 tggggttgtt caggcaaact catctgttgc actaacgttc cttggaactc ctcatggtct
     7141 aaccgaaatc tctccgagat ctgggataat atgacatggt tgcagtggga taaggagatt
     7201 agcaattaca ctcaaataat ctatgggctc ctggaggaaa gccagaatca gcaggagaag
     7261 aatgaacagg atcttctggc ccttgataag tgggcaagtc tttggaattg gttcgacatt
     7321 tcaaattggc tttggtatat aaagatattt ataatgattg tcgggggttt gataggtttg
     7381 aggattgtgt ttgctgtgct gtctgttatc cacagggtta ggcaggggta tagtccgctc
     7441 tcttttcaaa cccatacccc caaccccagg ggattggacc gacccgagcg gattgaggag
     7501 gaagacggtg aacaggaccg gggcagaagt acccgccttg ttagcgggtt tctggctctg
     7561 gcgtgggacg acttgcggag cctttgcctc ttctgctacc atcgcctcag agattttatc
     7621 ttgatcgctg ccagaatagt agaattgttg ggccacagct ccctcaaagg tttgagactt
     7681 ggatgggaag gtcttaaata cctgtggaat ttgctggctt actggggtag ggagctgaag
     7741 atttctgcca ttaacttgtt cgacacaata gctattgcag ttgcggagtg gactgacaga
     7801 gtcatagaaa taggccaaag attgtgtcgg gcgtttctcc acattccacg gcggattagg
     7861 caaggacttg aacgggccct cctttagtaa ctcgaggccg gcaaggccgg atccagacat
     7921 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     7981 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     8041 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     8101 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc
     8161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     8221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     8281 ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg
     8341 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     8401 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     8461 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     8521 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     8581 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     8641 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     8701 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     8761 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     8821 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     8881 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     8941 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     9001 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     9061 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     9121 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     9181 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     9241 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     9301 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     9361 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     9421 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     9481 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     9541 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     9601 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     9661 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     9721 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     9781 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     9841 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     9901 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     9961 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
    10021 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    10081 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
    10141 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
    10201 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
    10261 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
    10321 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
    10381 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
    10441 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
    10501 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
    10561 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
    10621 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
    10681 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
    10741 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
    10801 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
    10861 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
    10921 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
    10981 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
    11041 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
    11101 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
    11161 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    11221 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    11281 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    11341 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    11401 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    11461 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    11521 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    11581 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    11641 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    11701 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    11761 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    11821 gcccc
//
