LOCUS       sequence-240503-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multi-luciferase reporter vector including transcriptional reporters
            for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: MLRV
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    26..74
                     /note="synthetic polyadenylation signal"
     misc_feature    88..179
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        290..321
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    2006..2230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2243..2291
                     /note="synthetic polyadenylation signal"
     misc_feature    2305..2396
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        2507..2538
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2572..4224
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2638..2656)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4232..4456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4469..4517
                     /note="synthetic polyadenylation signal"
     misc_feature    4531..4622
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        4719..4750
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    5724..5948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5961..6009
                     /note="synthetic polyadenylation signal"
     misc_feature    6023..6114
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        6227..6258
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    6812..7036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7049..7097
                     /note="synthetic polyadenylation signal"
     misc_feature    7111..7202
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        7315..7346
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    8320..8544
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8617..8920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8921..9124
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9075..9095
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    10841..11065
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(11185..11773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11265..11284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11888..11990
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11991..12806
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             11991..12806
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12064..12083)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      12920..13348
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13007..13026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13217..13238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacaagctt cgtctcagtc aggagaataa aatatcttta ttttcattac atctgtgtgt
       61 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
      121 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctc
      181 gctgtcagga ggggaatttc cggggacttt ccgggaattt ccggggactt tccgggaatt
      241 tcctccctcg aggatatcaa atctggcctc ggcggccaag cttagacact agagggtata
      301 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accaatggag
      361 aacatggaga acgacgagaa cattgtggtg ggacccaagc cgttctaccc gatcgaggag
      421 ggttccgctg gcacccagct gcgcaagtac atggagcgct acgctaagct gggtgctatc
      481 gccttcacca acgccgtgac cggtgtggat tactcctacg ccgagtacct ggagaagtcc
      541 tgctgcctcg gaaaggccct gcagaactac ggtctggtcg tggatggccg cattgccctc
      601 tgctcggaga actgcgagga gttcttcatt cccgtgatcg ctggtctgtt catcggagtc
      661 ggcgttgccc caaccaacga gatctacacc ctgcgcgagc tggtccacag cctgggtatc
      721 agcaagccga ccatcgtttt ctcgagcaag aagggcctcg acaaggttat caccgtgcaa
      781 aaaaccgtca ccaccatcaa gaccatcgtt atcctggatt ccaaggttga ctaccgcggt
      841 taccagtgcc tcgacacctt catcaagcgt aacaccccac ccggcttcca agcctcctcc
      901 ttcaaaaccg tggaggttga tcgcaaggag caggtggccc tgatcatgaa ctcctccggc
      961 tccaccggtc tgccaaaggg cgtgcagctg acccacgaga acaccgtcac ccgcttctcg
     1021 catgcccgtg atcccatcta cggcaaccag gtgtccccag gcaccgccgt cctgaccgtc
     1081 gtgcccctcc accacggctt cggcatgttc accactctcg gctacttgat ctgcggcttc
     1141 cgtgtggtga tgctgactaa gttcgacgag gagactttcc tcaagaccct gcaagactac
     1201 aagtgcactt acgtgatcct ggtgccgacc ctgttcgcca tcctgaacaa gagcgagctg
     1261 ttgaacaaat acgacctgtc gaacctggtg gagatcgctt ccggcggcgc cccactgtcg
     1321 aaggaagttg gcgaggccgt tgctcgccgc ttcaacctgc ccggcgtccg tcagggttac
     1381 ggcctgaccg agactacctc ggctatcatc atcacccccg agggcgacga taagcccggc
     1441 gcttccggca aggttgttcc actcttcaaa gccaaggtga tcgacctgga caccaagaag
     1501 tccctgggtc ccaaccgtcg cggagaagtg tgcgtcaagg gccccatgct gatgaagggc
     1561 tacgtcaaca acccagaggc caccaaggag ttgatcgacg aggagggctg gctgcacact
     1621 ggtgacatcg gttactacga cgaagagaag cacttcttca tcgttgaccg cctgaagtcc
     1681 ttgatcaagt acaagggcta ccaggttccc cccgccgagc tcgagagcgt cctgctgcag
     1741 cacccctcca ttttcgatgc cggagtggcc ggtgtccccg atcccgtggc tggcgagctg
     1801 ccaggtgccg tcgtcgtgct ggagtccggc aagaacatga ccgagaagga agtgatggat
     1861 tacgtggcct cgcaagtgtc caacgccaag cgcctgcgcg gcggcgtgcg cttcgtggat
     1921 gaggtgccca agggcctcac cggaaagatc gacggtcgcg ctatccgcga gatcctcaag
     1981 aagcccgtgg ccaagatgtg agcttctgtg ccttctagtt gccagccatc tgttgtttgc
     2041 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2101 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2161 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2221 ggctctatgg cgctgtcagg agaataaaat atctttattt tcattacatc tgtgtgttgg
     2281 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
     2341 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctcgct
     2401 gtcaggaggt ctagatgccg tctagactta gtacgtctag actgccgtct agacggatcc
     2461 tccctcgagg atatcaaatc tggcctcggc ggccaagctt agacactaga gggtatataa
     2521 tggaagctcg acttccagct tggcaatccg gtactgttgg taaagccacc aatggaagac
     2581 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     2641 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     2701 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     2761 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     2821 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     2881 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     2941 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     3001 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     3061 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     3121 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     3181 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     3241 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     3301 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     3361 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     3421 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     3481 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     3541 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     3601 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     3661 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     3721 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     3781 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     3841 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     3901 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     3961 atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     4021 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     4081 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     4141 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     4201 aagggcggaa agatcgccgt gtaatgagct tctgtgcctt ctagttgcca gccatctgtt
     4261 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4321 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4381 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     4441 gcggtgggct ctatggcgct gtcaggagaa taaaatatct ttattttcat tacatctgtg
     4501 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     4561 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     4621 ctcgctgtca ggagcacgtg tgcacgtgga cacgtgctca cgtgctcacg tgtccctcga
     4681 ggatatcaaa tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct
     4741 cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccaatgactt ccaaggttta
     4801 tgaccccgag caacgcaaga gaatgataac tgggcctcag tggtgggcca gatgtaaaca
     4861 aatgaatgtc cttgatagtt tcatcaatta ctacgacagt gaaaagcatg cagaaaacgc
     4921 cgtaattttt ttgcatggca acgcagcctc ctcttatttg tggcgacacg tagtcccgca
     4981 tatagagccg gtagctaggt gtattatccc tgatctgatc ggaatgggaa agagtggcaa
     5041 gtcagggaat ggatcttatc gccttcttga ccactacaag tatctgacgg cttggttcga
     5101 actcttgaat ttgccgaaga aaatcatctt cgtgggtcac gattggggag cctgtctcgc
     5161 gttccattat tcctacgagc atcaggacaa gataaaggca atcgttcacg ccgagtctgt
     5221 agtagatgta atcgagtcct gggacgaatg gccagatatt gaggaggata tagcattgat
     5281 taaaagcgag gaaggggaaa agatggtgct ggagaacaac ttctttgtgg aaacaatgct
     5341 tccttcaaaa atcatgagaa aactggaacc cgaggaattt gccgcttatc tcgaaccgtt
     5401 taaagaaaag ggagaagtcc gcaggccaac actttcctgg ccaagagaaa tcccgctcgt
     5461 caaaggcgga aagccggatg ttgtgcaaat agtccgaaat tacaacgcgt acctccgggc
     5521 ctctgacgat ctccctaaaa tgtttataga atcagatcca ggatttttta gcaacgctat
     5581 cgtagaagga gcaaagaaat ttccaaatac cgaatttgtc aaagtaaagg gactgcattt
     5641 cagtcaagag gacgctccag acgagatggg caaatacata aagtcctttg tagagagggt
     5701 cctgaagaac gaacagtgag cttctgtgcc ttctagttgc cagccatctg ttgtttgccc
     5761 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5821 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5881 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5941 ctctatggcg ctgtcaggag aataaaatat ctttattttc attacatctg tgtgttggtt
     6001 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
     6061 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctcgctgt
     6121 caggagtaca gaacatgtct aagcatgctg tgccttgcct ggacttgcct ggccttgcct
     6181 tccctcgagg atatcaaatc tggcctcggc ggccaagctt agacactaga gggtatataa
     6241 tggaagctcg acttccagct tggcaatccg gtactgttgg taaagccacc aatggtgttt
     6301 accttggagg acttcgtggg tgactggagg caaactgcag gatacaattt ggaccaggtc
     6361 ctcgaacagg gaggagtcag cagccttttc caaaacttgg gcgtgtccgt tacccctatt
     6421 cagagaattg tcctttccgg agagaacgga ttgaaaatag atatccacgt cataatcccg
     6481 tatgaaggac tgtctgggga ccaaatgggt cagattgaaa aaatattcaa agtagtttat
     6541 cccgttgatg atcatcactt caaagttatc cttcattatg ggactttggt aatcgatgga
     6601 gttactccta atatgataga ttactttggc cgaccttacg aggggatcgc tgtattcgac
     6661 ggcaagaaaa tcaccgtcac tggaaccctc tggaacggca acaagattat tgacgagcga
     6721 ttgatcaatc cagacggatc tttgcttttc cgcgtaacga taaacggcgt aacagggtgg
     6781 aggctctgcg aacgaatact cgcctgagct tctgtgcctt ctagttgcca gccatctgtt
     6841 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6901 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6961 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7021 gcggtgggct ctatggcgct gtcaggagaa taaaatatct ttattttcat tacatctgtg
     7081 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     7141 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     7201 ctcgctgtca ggagtgagtc agtgactcag tgagtcagtg actcagtgag tcagtgactc
     7261 aggcatgctc cctcgaggat atcaaatctg gcctcggcgg ccaagcttag acactagagg
     7321 gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccaa
     7381 tggcttccaa agtctacgac ccagaacaaa ggaagaggat gattactgga ccgcagtggt
     7441 gggcaaggtg caagcagatg aatgtactcg atagttttat caattattac gactccgaaa
     7501 aacacgcgga aaatgctgtc atattcctgc acgggaacgc cactagcagc tacttgtggc
     7561 ggcacgtagt cccccacata gagcctgttg cccgatgtat tattcccgat ctcattggaa
     7621 tgggcaaatc aggcaaatct gggaacggtt cttaccgcct gctcgatcac tataaatatt
     7681 tgaccgcttg gtttgagctg cttaatctcc ctaaaaaaat aatattcgtt ggacacgact
     7741 ggggctcagc tctggcgttc cactacgcat acgagcatca agaccgaata aaagcaatag
     7801 tacatatgga gtcagttgtt gacgtcatag agtcatggat gggttggcca gatattgaag
     7861 aggaattggc cctcatcaag agtgaggagg gggaaaaaat ggtcctggaa aataattttt
     7921 tcgtggaaac cttgctcccg agtaaaataa tgcgaaaact tgaacccgaa gagtttgctg
     7981 cttatttgga gcctttcaag gaaaaggggg aagtaagacg accaaccctt tcttggccga
     8041 gggagatacc tcttgttaag ggtggaaagc ccgacgtagt ccagatagtc cgaaattaca
     8101 acgcgtacct gagagctagt gatgacctcc ctaagttgtt tatcgagtca gaccctgggt
     8161 tcttcagcaa cgcaattgta gaaggagcga aaaaatttcc aaatacagaa tttgtgaagg
     8221 ttaaggggct ccactttttg caagaggacg cccccgatga gatgggaaaa tacattaaat
     8281 ctttcgtaga gcgcgtcctt aagaatgaac agtgagcttc tgtgccttct agttgccagc
     8341 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     8401 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     8461 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     8521 ctggggatgc ggtgggctct atggcgctgt caggagtagt tattaatagt aatcaattac
     8581 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     8641 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     8701 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     8761 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     8821 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     8881 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     8941 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9001 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9061 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9121 agctggttta gtgaaccgtc agatcaagct caaatgatga agagagagaa gaatgttgtg
     9181 tatgggcccg agcctaagca cccgcttggg aattttaccg ctggagagat gctctacaac
     9241 gcgcttcaca aacatagtca catcccgcag gcgatcctcg acgtgatggg taacgaaagt
     9301 cttagttatc aggaattctt cgacacgacg gtcaaattgg ggcagagtct ccagaattgt
     9361 ggctataaaa tgaatgatgt tgtatcaata tgcgctgaaa acaacaaaag atttttcatt
     9421 ccaattatta gtgcgtggta tataggcatg gtcgttgcac ctgtaaacga ggactatata
     9481 ccagacgaac tgtgtaaggt cacaggaatt agtaagccaa tacttgtgtt caccacgcga
     9541 aagatacttc caaaggtttt ggaggttaag gatagaacaa attatatcaa aagaatcata
     9601 attctggact ctgaggaaaa ccttctcgga tgtgaatcct tgcataattt tatgtcaaga
     9661 tatagcgata acaatcttca gacctttaaa ccactgcatt acgatccggt cgaccaggtt
     9721 gcagcgatac tgtgtagtag tggtactacc gggttgccta agggcgtcat gcagacccac
     9781 cgcaatatat gtgttcggct gacccacgcc tctgacccga gagttggtac ccaactcatt
     9841 cccggagtta gtgtgctggc gtatcttccc ttctttcacg cctttgggtt ttccattaac
     9901 ttggggtatt ttatggtggg gctgcgcgtc gtgatgcttc ggaggttcaa ccaagaggtt
     9961 tttttgaagg cgattcaaga ctatgaagta cgaagtgtga tcaatgtccc aagtacaatc
    10021 ctctttcttt ctaaatcacc gttggttgat aagtatgatc tttctacctt ggcggaactt
    10081 tgttgtggtg cggcacctct tgcaaaagaa gtagctgaaa tcgccgtaaa acgccttaat
    10141 ttgccaggca tacggtgtgg ttacgggttg actgaaagca catccgctaa tatccacact
    10201 cttcacaacg aatttaaatc cggttcattg ggaaaagtga cgccctacat ggccgctaaa
    10261 attatagatc gaaacaccgg agaggcgctt ggcccaaatc aagtgggaga gttgtgcatt
    10321 tggggcccca tggtcacgaa aggctacgtg aataatccac aggccaccaa ggaggccatt
    10381 gacgacgatg gttggcttca cagtggtgat tttggctact atgatgagga tgaatatttt
    10441 tacattgtgg atagatataa agagctgatc aagtacaagg gatatcaagt ggctcctgtc
    10501 gagttggagg agattttgct ccaacatcca gggatccgcg atgtggccgt agtcggtatc
    10561 cccgacatag aagcagggga acttccggcc ggattcgttg tcaagcaacc tggggcccaa
    10621 ctgacggcta aagaagtata tgatttcctt gcacaaagag ttagccacag caagtatctt
    10681 cgagggggtg tcagattcgt tgactctatt ccgaggaacg tcacgggcaa aatcagccga
    10741 aaggaactcc gagaagcact tatggagaag gcgggtggtg gatctatgta caagacatcc
    10801 gccgccaacg atgagaacta tgccctggcg gcttgagctt ctgtgccttc tagttgccag
    10861 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    10921 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    10981 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
    11041 gctggggatg cggtgggctc tatggcgctt gagacgaagc ttgacacggt tatccacaga
    11101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11821 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11881 tttgtgatga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
    11941 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata
    12001 ttcaacggga aacgtcttgc tcaaggccgc gattaaattc caacatggat gctgatttat
    12061 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc taccgattgt
    12121 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
    12181 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
    12241 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga
    12301 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
    12361 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
    12421 atcgcgtatt tcgactcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
    12481 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
    12541 aacttttgcc attctcaccg gattcagtag tcactcatgg tgatttctca cttgataacc
    12601 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
    12661 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
    12721 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
    12781 atttgatgct cgatgagttt ttctgatttt ttttaaggca gttattggtg cccttaaacg
    12841 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgatgata
    12901 agctgtcaaa catgatgtta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    12961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    13021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    13081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    13141 gatggttcac gtagtgggcc agcgccctga tagacggttt ttcgcccttt gacgttggag
    13201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    13261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    13321 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aattt
//
