LOCUS       sequence-240530-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies
            of HBB 3' UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun
            D, Yoo KW, Atala ZP, Lyu P, Atala A
  TITLE     Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for 
            transient expression and efficient genome editing.
  JOURNAL   Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 
            10.1093/nar/gkz093.
  PUBMED    30759231
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093."
COMMENT     Sequence Label: pSaCas9-1xPP7-2x3'UTR
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(403..420)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    409..616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        626..875
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     798..817
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tcatcnnnng ggcnnnggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
       61 ggatcctaag ctcgctttct tgctgtccaa tttctattaa aggttccttt gttccctaag
      121 tccaactact aaactggggg atattatgaa gggccttgag catctggatt ctgcctaata
      181 aaaaacattt attttcattg ctagctcgct ttcttgctgt ccaatttcta ttaaaggttc
      241 ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg
      301 gattctgcct aataaaaaac atttattttc attgcgaatt cggagcagac gatatggcgt
      361 cgctccgtcg actctagaaa attcctagag ctcgctgatc agcctcgact gtgccttcta
      421 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      481 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
      541 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
      601 gcaggcatgc tggggaggta cggccgangg cctatttccc atgattcctt catatttgca
      661 tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
      721 attagtacaa aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
      781 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
      841 tggntttata tatcttgtgg aaaggacgaa acaccgagta acggcanact tctccacgtt
      901 nctagtactn ctggnaaaca gatctactan aacanggcaa aatgccgtgt ttatnncgtc
      961 aacttgttgc nananatcgg agcagacgan ntggcgtcgc tncnncgctc gctttcntgc
     1021 tgtccnattt cta
//
